| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,666,745 | A→T | 7.7% | intergenic (+19/‑47) | acnA‑II → / → prpF | aconitate hydratase/aconitate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,666,745 | 0 | A | T | 7.7% | 126.8 / 3.2 | 52 | intergenic (+19/‑47) | acnA‑II/prpF | aconitate hydratase/aconitate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (22/24); new base T (2/2); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-02 | |||||||||||
GTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGCCTGCAAGACCTGCCCGAGCAGGCG > NC_002947/2666599‑2666890 | gTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCaa > 4:320459/1‑148 (MQ=255) cAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCCGCGCTTTGCCCAGGACATC‑CTCGAAGCGACCGCCAGAGC‑ATGAATCGGGGGCCCGAAAGccg > 8:354875/1‑147 (MQ=255) cAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGCCTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCgg > 5:196648/1‑149 (MQ=255) gAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAgg > 1:90459/1‑149 (MQ=255) tGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTa > 3:164447/1‑149 (MQ=255) ccTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGc > 5:304937/1‑138 (MQ=255) ccTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGc < 6:304937/138‑1 (MQ=255) cTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTAttt > 8:331753/1‑149 (MQ=255) ggACCTGCCGGCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGCTATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATc < 1:89594/148‑1 (MQ=255) cTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGAt > 7:209648/1‑149 (MQ=255) tGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGGCCAGCGGCCCCCCGGGTTTTGACCCGACGATGAGGCTg > 5:52111/1‑149 (MQ=255) cGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGccc < 8:209648/149‑1 (MQ=255) cGTCTGGACACCGCCGAGGAAGTGGCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGccc < 5:111750/149‑1 (MQ=255) cacCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAAt < 4:128143/149‑1 (MQ=255) cacCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAAt < 3:320459/149‑1 (MQ=255) cacCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAAt < 2:90459/149‑1 (MQ=255) cgccgAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTc < 7:331753/149‑1 (MQ=255) cgccgAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGACCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCACGGTATTTAACCGGACGATAAGGATGACCAAGAAAAAGt > 3:134238/1‑148 (MQ=255) ccgAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGTGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTca < 2:227109/148‑1 (MQ=255) ccgAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTca > 1:227109/1‑148 (MQ=255) ccgAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCGTTGCCCAGGACTTC‑CTCGACGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTcac < 6:307258/149‑1 (MQ=255) cgAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTcaca < 7:284801/149‑1 (MQ=255) gAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTCGCTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAACCCGCCCCAGGGTATTTCACCCGACAATCAGGATGCCCAAGAAAATGtaaa > 1:104249/1‑145 (MQ=255) gtCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCACGGTATTTCACCGGACAATAAGGATGCCCAAGAAAATGTCACATTTACCCCa > 7:14808/1‑149 (MQ=255) gATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGa < 1:177932/148‑1 (MQ=255) tCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGCACGCCTGAGC‑AGGAATCGGGGGCGGCAAAGGGGCCCCACGGTATTTAACCCGGCTATAAGGCTGCGCAAGAAAATGTGACAGGGAACGCAGATc > 6:28732/1‑148 (MQ=255) aCGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTGACCGGACTATCAGGATGGCCAAGAAAATGTCACATGTACCCCAGATCaaa > 3:101060/1‑148 (MQ=255) tggTGCTTCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCAGAGC‑ATGAATCGGGCTCCGCAAAGCGGCCCCAGTGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGcctcct < 6:98866/148‑1 (MQ=255) aGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCcgcg < 2:197437/149‑1 (MQ=255) cTTTGCCCAGGACTTC‑CTCGAGGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGcggcgg < 3:349881/149‑1 (MQ=255) cTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGGATTTGACCGGGCTATGAGGCTGGCCCAGCAAATGTGACATGTAGCCCAGAGCAAAATACCCGCCACCGACAACCCcggcgg > 4:285658/1‑149 (MQ=255) tttGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCCGCCCCAGGGTATTTGACCGGCCAATCAGGATGCCCAAAAAAATGTCAAATGTACCCCAGAAAAAAAACCCCCCCCCCAACATACCCcgcggc > 4:132676/1‑149 (MQ=255) ccAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCCAAACGGCCCCAGGGGATTTCACCGGACTATCACGATTCCCAAAAAAAATAAAAAAGTAACCCACATAAAAATACCCCCCACCAACAAACCCGGCGGCGCCac > 4:7688/1‑148 (MQ=255) gACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCCAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCaaa < 3:191438/149‑1 (MQ=255) gACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCaaa < 6:318805/149‑1 (MQ=255) acctc‑ctccAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCaaa < 1:325964/145‑1 (MQ=255) ttC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGCCTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGc > 5:297026/1‑149 (MQ=255) c‑cTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTGCCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGc < 2:104249/147‑1 (MQ=255) c‑cTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCg < 6:196648/148‑1 (MQ=255) c‑cTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTAAAAGGATGCCCAAGAAAATGTGACATGTACCCCAGATAAAAATACCCGCCCCCTACATCCGCGGCGGCACCAGCAAAGGCgg > 6:80088/1‑148 (MQ=255) cGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCgtgt > 5:81038/1‑148 (MQ=255) aTGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGCCTGCAAGACCt < 4:164447/149‑1 (MQ=255) aTGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTAACCGGACTTTGAGGATGCCCAAGAAAATGTGACATGTAACCCAGATCAAAATGCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGCCTGCAAGACCt > 2:80021/1‑149 (MQ=255) gAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAg < 5:329856/117‑1 (MQ=255) aaTCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGCCTGCAAGACCTGcc < 8:14808/149‑1 (MQ=255) cctggggCCGCTTTGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGcggcgg < 6:286510/105‑1 (MQ=255) cctggggCCGCTTTGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGcggcgg > 5:286510/4‑108 (MQ=255) tggggCCGCTTTGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCgcggc < 7:302973/103‑1 (MQ=255) tggggCCGCTTTGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCgcggc > 8:302973/2‑104 (MQ=255) ggCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGttct < 7:327274/123‑1 (MQ=255) ggCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGttct > 8:327274/1‑123 (MQ=255) cGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGCCTGCAAGACCTGCCCGAGCAGgcg > 6:196006/1‑149 (MQ=255) gCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGCCTGCAAGACCTGCCCGAGCAGgcg > 7:23824/1‑148 (MQ=255) | GTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGACTTC‑CTCGAAGCGACCGCCTGAGC‑ATGAATCGGGGGCCGCAAAGCGGCCCCAGGGTATTTCACCGGACTATCAGGATGCCCAAGAAAATGTCACATGTACCCCAGATCAAAATCCCCGCCACCTACATCCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGCCTGCAAGACCTGCCCGAGCAGGCG > NC_002947/2666599‑2666890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |