Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,673,830 G→T 7.3% intergenic (‑20/+2) PP_5519 ← / ← PP_2342 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,673,8300GT 7.3% 151.7 / 3.9 55intergenic (‑20/+2)PP_5519/PP_2342hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-02

GCGTATCTTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGAATAAT  >  NC_002947/2673682‑2673976
                                                                                                                                                    |                                                                                                                                                  
gcgTATCTTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCg                                                                                                                                                    <  6:212139/149‑1 (MQ=255)
gcgTATCTTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCg                                                                                                                                                    <  6:279198/149‑1 (MQ=255)
  gTATCTTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAATTACAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGt                                                                                                                                                  >  3:218527/1‑149 (MQ=255)
  gTATCTTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGt                                                                                                                                                  >  4:164373/1‑149 (MQ=255)
       ttAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGc                                                                                                                                             >  1:160885/1‑149 (MQ=255)
       gtAACCTGTTTTGTAAAAGCGCCCTCACGGATTCCGCGATAGGCGGGGTCCCGGTCTATCCACGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGGCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGc                                                                                                                                             >  6:330848/2‑149 (MQ=255)
           ccTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCtt                                                                                                                                          >  2:319198/1‑148 (MQ=255)
            cTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccata                                                                                                                                         >  2:43167/1‑145 (MQ=255)
            cTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCtt                                                                                                                                          >  4:18602/1‑147 (MQ=255)
               ttttGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccatataa                                                                                                                                      <  1:43167/148‑7 (MQ=255)
                   gTAACCGCGACCTCACGGATTCCGCGATAGGCTGTGTCCCTGTCTATGGTCGTTAAGTACAGCAACTTGTCGAGGCGCAGGTAGAGGTCGAGGTGTGCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGttt                                                                                                                                  <  4:218527/148‑1 (MQ=255)
                   gTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGtt                                                                                                                                  <  2:88432/148‑1 (MQ=255)
                       aCGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTg                                                                                                                             >  7:184536/1‑149 (MQ=255)
                               tCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCt                                                                                                                                           >  1:95241/1‑127 (MQ=255)
                               tCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCt                                                                                                                                           <  2:95241/127‑1 (MQ=255)
                               tCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAg                                                                                                                     >  1:301102/1‑149 (MQ=255)
                                   ggATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTa                                                                                                                  >  8:322663/1‑148 (MQ=255)
                                       tCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCt                                                                                                             >  5:69967/1‑149 (MQ=255)
                                         cgcgATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccatataa                                                                                                                                      >  4:63589/1‑116 (MQ=255)
                                         cgcgATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccatataa                                                                                                                                      <  3:63589/122‑7 (MQ=255)
                                         cgcgATAGGCGGTGTCCCGGTCTATACTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGATCTGCATGTCGCATGGGCTTCTCTAATATGGCCCGTCAGGCCTTGAGCGGTTCACTGCCTGCCAGGTACAGCTg                                                                                                            >  8:25786/1‑148 (MQ=255)
                                          gcgATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTg                                                                                                            >  4:152264/1‑147 (MQ=255)
                                              tAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCt                                                                                                      <  7:322663/149‑1 (MQ=255)
                                               aGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTg                                                                                                     >  4:321127/1‑149 (MQ=255)
                                                ggCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTg                                                                                                     <  2:301102/148‑1 (MQ=255)
                                                    gtgtCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACtt                                                                                                <  2:142843/149‑1 (MQ=255)
                                                      gtCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGc                                                                                              <  3:69524/149‑1 (MQ=255)
                                                         ccGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTg                                                                                           >  1:144286/1‑149 (MQ=255)
                                                         ccGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTg                                                                                           >  7:167446/1‑149 (MQ=255)
                                                               tATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATg                                                                                      >  5:315098/1‑148 (MQ=255)
                                                               tATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATg                                                                                      >  2:31235/1‑148 (MQ=255)
                                                                   cTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATgaagaa                                                                                 >  8:214264/1‑149 (MQ=255)
                                                                                 caACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCgg                                                                   <  8:130741/149‑1 (MQ=255)
                                                                                 caACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCgg                                                                   <  7:307564/149‑1 (MQ=255)
                                                                                  aaCATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGt                                                                  >  6:371120/1‑149 (MQ=255)
                                                                                    cATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGGCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGa                                                                <  8:59583/149‑1 (MQ=255)
                                                                                             gcgcAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCgg                                                       <  3:164373/149‑1 (MQ=255)
                                                                                              cgcAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCggg                                                      <  8:184536/149‑1 (MQ=255)
                                                                                                 aGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTAc                                                   <  1:319198/149‑1 (MQ=255)
                                                                                                    aaGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAga                                                <  3:18602/149‑1 (MQ=255)
                                                                                                       cGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAgattc                                             <  6:69967/149‑1 (MQ=255)
                                                                                                                tgtTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCt                                    <  2:160885/149‑1 (MQ=255)
                                                                                                                  ttCTGCATGTCGAATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCTGCCATGTACAGCTGTAGGCTTGACTTGCGTGCCATGAAGAACGAGAGCTTGGCGGTGATAGCTGCGGGTACAGATTGGATTCGTCTg                                   <  7:25786/148‑1 (MQ=255)
                                                                                                                  ttCAGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTg                                   <  6:315098/148‑1 (MQ=255)
                                                                                                                         tGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTccc                           <  5:330848/149‑1 (MQ=255)
                                                                                                                              cATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCctc                      <  5:371120/149‑1 (MQ=255)
                                                                                                                              cATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCctc                      >  7:325307/1‑149 (MQ=255)
                                                                                                                              cATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCctc                      >  7:320265/1‑149 (MQ=255)
                                                                                                                                    cTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTgg                <  7:214264/149‑1 (MQ=255)
                                                                                                                                       ctctTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTg             >  4:50220/1‑149 (MQ=255)
                                                                                                                                        tctTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGc            >  7:248679/1‑149 (MQ=255)
                                                                                                                                            atatGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGGCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCg           <  8:167446/146‑1 (MQ=255)
                                                                                                                                                ggCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTg                                                                       <  5:254380/82‑1 (MQ=255)
                                                                                                                                                ggCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTg                                                                       >  6:254380/1‑82 (MQ=255)
                                                                                                                                                 gCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGaat     <  1:339575/147‑1 (MQ=255)
                                                                                                                                                 gCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGaat     >  2:339575/1‑147 (MQ=255)
                                                                                                                                                  ccGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGaataat  <  3:321127/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGTATCTTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGAATAAT  >  NC_002947/2673682‑2673976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑