Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,673,834 A→C 7.4% *65G (TGA→GGA)  PP_2342 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,673,8340AC 7.4% 158.9 / 4.0 54*65G (TGA→GGA) PP_2342hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/25);  new base C (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

TTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGAATAATGCTG  >  NC_002947/2673689‑2673980
                                                                                                                                                 |                                                                                                                                                  
ttAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGc                                                                                                                                                 >  1:160885/1‑149 (MQ=255)
gtAACCTGTTTTGTAAAAGCGCCCTCACGGATTCCGCGATAGGCGGGGTCCCGGTCTATCCACGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGGCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGc                                                                                                                                                 >  6:330848/2‑149 (MQ=255)
    ccTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCtt                                                                                                                                              >  2:319198/1‑148 (MQ=255)
     cTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccata                                                                                                                                             >  2:43167/1‑145 (MQ=255)
     cTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCtt                                                                                                                                              >  4:18602/1‑147 (MQ=255)
        ttttGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccatataa                                                                                                                                          <  1:43167/148‑7 (MQ=255)
            gTAACCGCGACCTCACGGATTCCGCGATAGGCTGTGTCCCTGTCTATGGTCGTTAAGTACAGCAACTTGTCGAGGCGCAGGTAGAGGTCGAGGTGTGCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGttt                                                                                                                                      <  4:218527/148‑1 (MQ=255)
            gTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGtt                                                                                                                                      <  2:88432/148‑1 (MQ=255)
                aCGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTg                                                                                                                                 >  7:184536/1‑149 (MQ=255)
                        tCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCt                                                                                                                                               >  1:95241/1‑127 (MQ=255)
                        tCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCt                                                                                                                                               <  2:95241/127‑1 (MQ=255)
                        tCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAg                                                                                                                         >  1:301102/1‑149 (MQ=255)
                            ggATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTa                                                                                                                      >  8:322663/1‑148 (MQ=255)
                                tCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCt                                                                                                                 >  5:69967/1‑149 (MQ=255)
                                  cgcgATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccatataa                                                                                                                                          >  4:63589/1‑116 (MQ=255)
                                  cgcgATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCTGACCGGccatataa                                                                                                                                          <  3:63589/122‑7 (MQ=255)
                                  cgcgATAGGCGGTGTCCCGGTCTATACTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGATCTGCATGTCGCATGGGCTTCTCTAATATGGCCCGTCAGGCCTTGAGCGGTTCACTGCCTGCCAGGTACAGCTg                                                                                                                >  8:25786/1‑148 (MQ=255)
                                   gcgATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTg                                                                                                                >  4:152264/1‑147 (MQ=255)
                                       tAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCt                                                                                                          <  7:322663/149‑1 (MQ=255)
                                        aGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTg                                                                                                         >  4:321127/1‑149 (MQ=255)
                                         ggCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTg                                                                                                         <  2:301102/148‑1 (MQ=255)
                                             gtgtCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACtt                                                                                                    <  2:142843/149‑1 (MQ=255)
                                               gtCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGc                                                                                                  <  3:69524/149‑1 (MQ=255)
                                                  ccGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTg                                                                                               >  7:167446/1‑149 (MQ=255)
                                                  ccGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTg                                                                                               >  1:144286/1‑149 (MQ=255)
                                                        tATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATg                                                                                          >  2:31235/1‑148 (MQ=255)
                                                        tATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATg                                                                                          >  5:315098/1‑148 (MQ=255)
                                                            cTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATgaagaa                                                                                     >  8:214264/1‑149 (MQ=255)
                                                                          caACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCgg                                                                       <  8:130741/149‑1 (MQ=255)
                                                                          caACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCgg                                                                       <  7:307564/149‑1 (MQ=255)
                                                                           aaCATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGt                                                                      >  6:371120/1‑149 (MQ=255)
                                                                             cATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGGCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGa                                                                    <  8:59583/149‑1 (MQ=255)
                                                                                      gcgcAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCgg                                                           <  3:164373/149‑1 (MQ=255)
                                                                                       cgcAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCggg                                                          <  8:184536/149‑1 (MQ=255)
                                                                                          aGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTAc                                                       <  1:319198/149‑1 (MQ=255)
                                                                                             aaGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAga                                                    <  3:18602/149‑1 (MQ=255)
                                                                                                cGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAgattc                                                 <  6:69967/149‑1 (MQ=255)
                                                                                                         tgtTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCt                                        <  2:160885/149‑1 (MQ=255)
                                                                                                           ttCTGCATGTCGAATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCTGCCATGTACAGCTGTAGGCTTGACTTGCGTGCCATGAAGAACGAGAGCTTGGCGGTGATAGCTGCGGGTACAGATTGGATTCGTCTg                                       <  7:25786/148‑1 (MQ=255)
                                                                                                           ttCAGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTg                                       <  6:315098/148‑1 (MQ=255)
                                                                                                                  tGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTccc                               <  5:330848/149‑1 (MQ=255)
                                                                                                                       cATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCctc                          <  5:371120/149‑1 (MQ=255)
                                                                                                                       cATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCctc                          >  7:320265/1‑149 (MQ=255)
                                                                                                                       cATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCctc                          >  7:325307/1‑149 (MQ=255)
                                                                                                                             cTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTgg                    <  7:214264/149‑1 (MQ=255)
                                                                                                                                ctctTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTg                 >  4:50220/1‑149 (MQ=255)
                                                                                                                                 tctTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGc                >  7:248679/1‑149 (MQ=255)
                                                                                                                                     atatGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGGCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCg               <  8:167446/146‑1 (MQ=255)
                                                                                                                                         ggCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTg                                                                           >  6:254380/1‑82 (MQ=255)
                                                                                                                                         ggCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTg                                                                           <  5:254380/82‑1 (MQ=255)
                                                                                                                                          gCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGaat         <  1:339575/147‑1 (MQ=255)
                                                                                                                                          gCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGaat         >  2:339575/1‑147 (MQ=255)
                                                                                                                                           ccGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGaataat      <  3:321127/149‑1 (MQ=255)
                                                                                                                                              gTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGAt                                                                   >  3:200851/1‑85 (MQ=255)
                                                                                                                                                 aGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGAATAAtgctg  >  5:11052/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTAACCTGTTTTGTAAACGCGCCCTCACGGATTCCGCGATAGGCGGTGTCCCGGTCTATCCTCGTCAAGTGCAGCAACATGTCGAGGCGCAGGAAGCGGTCGAGGTGTTCTGCATGTCGCATGGGCTTCTCTTATATGGCCGGTCAGGCCTTGAGCGGTTCACTGCCGGCCAGGTACAGCTGCAGGCTGGACTTGCGTGCCATGAAGAACGAGACCTTGGCGGTGATAGCTGCGGGTACAGATTCGATTCGTCTGCTCGTCCCGCCTCTCCTGGGTGCGGAGAATAATGCTG  >  NC_002947/2673689‑2673980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑