Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	288,667	A→T	5.2%	K29M (AAG→ATG)	oprE →	outer‑membrane porin E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	288,667	0	A	T	5.2%	245.1 / 2.3	77	K29M (AAG→ATG)	oprE	outer‑membrane porin E
Reads supporting (aligned to +/- strand):  ref base A (34/39);  new base T (2/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

TGAGCGGGGCATCAGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGATGTTCAAGC  >  NC_002947/288519‑288814                                                                                                                                                      |                                                                                                                                                    tGAGCGGGGCATCAGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCaa                                                                                                                                                     <  4:121616/149‑1 (MQ=255)  gAGCGGGGCATCAGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTGTCGCCCACCAAGCAGGCGCTGCCGGGTTCGTTGAG‑GACAGCAAg                                                                                                                                                    >  3:54083/1‑149 (MQ=255)    gCGGGGCATCAGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGTTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCt                                                                                                                                                  <  4:145277/149‑1 (MQ=255)       gggCATCAGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTCGCTCTGGACGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGGTGCCGGTTTCGTTGAG‑GACAGCAAGCTgac                                                                                                                                               >  4:364564/1‑147 (MQ=255)            tCAGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACAGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCa                                                                                                                                          <  8:312636/149‑1 (MQ=255)              aGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAGCGACAGCTTGCTGTcctcaac                                                                                                                                         <  7:310948/149‑7 (MQ=255)              aGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGc                                                                                                                                        >  6:14807/1‑149 (MQ=255)               gACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCt                                                                                                                                       >  4:194779/1‑149 (MQ=255)                         ccATAGATACAGAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCAt                                                                                                                             <  7:325633/149‑1 (MQ=255)                         ccAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGACCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCAt                                                                                                                             >  5:60280/1‑149 (MQ=255)                                   acaAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAAc                                                                                                                   >  2:27805/1‑149 (MQ=255)                                    caAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTcgcg                                                                                                                                   <  8:252729/132‑1 (MQ=255)                                    caAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTcgcg                                                                                                                                   >  7:252729/1‑132 (MQ=255)                                    caAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACg                                                                                                                  <  4:212694/149‑1 (MQ=255)                                       aaaTTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGAt                                                                                                                >  8:123620/1‑148 (MQ=255)                                             gAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAAcc                                                                                                         >  5:85328/1‑149 (MQ=255)                                                     aggagCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGc                                                                                                 <  8:32074/149‑1 (MQ=255)                                                      ggagCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCg                                                                                                <  1:201154/149‑1 (MQ=255)                                                        agCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGt                                                                                              <  2:199219/149‑1 (MQ=255)                                                              aCATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGAc                                                                                        <  6:85328/149‑1 (MQ=255)                                                              aCATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGAc                                                                                        <  7:123620/149‑1 (MQ=255)                                                               cATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCg                                                                                                >  5:41616/1‑140 (MQ=255)                                                               cATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCg                                                                                                <  6:41616/140‑1 (MQ=255)                                                               cATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGAcc                                                                                       <  5:14807/149‑1 (MQ=255)                                                                    aCAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCagaa                                                                                   >  8:154065/1‑148 (MQ=255)                                                                    aCAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCaga                                                                                    >  1:245132/1‑147 (MQ=255)                                                                     cAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAGCGATGACAACCGCGAAAGCGGTATTGACCaga                                                                                    >  8:247733/1‑146 (MQ=255)                                                                        gTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCTCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAgaaagaaa                                                                              <  3:242186/149‑1 (MQ=255)                                                                        gTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAgaaagaaa                                                                              <  6:184453/149‑1 (MQ=255)                                                                           cAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGt                                                                            <  7:79962/148‑1 (MQ=255)                                                                           cAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTg                                                                           >  4:56229/1‑149 (MQ=255)                                                                            aGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTgg                                                                          <  7:10895/149‑1 (MQ=255)                                                                            aGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTgg                                                                          >  3:304468/1‑149 (MQ=255)                                                                             gCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCTAAAGCGGTATTGACCAGAAAGAAAGTGGt                                                                         <  1:27805/149‑1 (MQ=255)                                                                              ccTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGt                                                                         >  2:265501/1‑148 (MQ=255)                                                                              ccTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTc                                                                        <  2:339258/149‑1 (MQ=255)                                                                                  gCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAggg                                                                    <  7:154065/149‑1 (MQ=255)                                                                                  gCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAgg                                                                     <  5:45290/148‑1 (MQ=255)                                                                                    tctGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTCACCAGAAAGAAAGTGGTCAGGGtt                                                                  >  5:292633/1‑149 (MQ=255)                                                                                          cGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACAAGAAAGAAAGTGTTCAGGGTTTCAAGc                                                            >  4:56378/1‑149 (MQ=255)                                                                                             ggCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAgg                                                                     <  4:111848/137‑1 (MQ=255)                                                                                             ggCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAgg                                                                     >  3:111848/1‑137 (MQ=255)                                                                                               gaCTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGTTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGAc                                                       <  4:54083/148‑1 (MQ=255)                                                                                                    ggggTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTAc                                                    <  7:293249/147‑1 (MQ=255)                                                                                                       gTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTc                                                                        <  6:71718/124‑1 (MQ=255)                                                                                                       gTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTc                                                                        >  5:71718/1‑124 (MQ=255)                                                                                                         tctcGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGATCGACTACCTGTCTg                                             >  5:240160/1‑149 (MQ=255)                                                                                                          atcGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGAACCATGTACATCAACTATGACAACCGCGAAAGAGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAATCTCGACAACCTGTCTg                                             >  5:96635/2‑148 (MQ=255)                                                                                                           tcGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGaaagaaag                                                                             >  3:76969/1‑115 (MQ=255)                                                                                                           tcGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGaaagaaag                                                                             <  4:76969/115‑1 (MQ=255)                                                                                                             gCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCtt                                         <  1:265501/149‑1 (MQ=255)                                                                                                             gCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCtt                                         <  7:294469/149‑1 (MQ=255)                                                                                                               ccAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTTGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCa                                       >  3:372981/1‑149 (MQ=255)                                                                                                               ccAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTc                                        >  6:205086/1‑148 (MQ=255)                                                                                                                 aacaaGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACt                                     >  2:49522/1‑149 (MQ=255)                                                                                                                  acaaGCATGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTc                                    <  3:29851/149‑1 (MQ=255)                                                                                                                     aGCAGTCGCTGCCTGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACGATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAg                                 <  6:374729/149‑1 (MQ=255)                                                                                                                     aGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCTAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAg                                 <  6:60280/149‑1 (MQ=255)                                                                                                                      gCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAgg                                >  3:272336/1‑149 (MQ=255)                                                                                                                       cAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAgg                                                                     >  8:94607/1‑111 (MQ=255)                                                                                                                       cAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAgg                                                                     <  7:94607/111‑1 (MQ=255)                                                                                                                              tGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAg                        >  8:272618/1‑149 (MQ=255)                                                                                                                                  ggTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACGGCGAAAGCGGTATTGACCAGTAAGAAAGTGGTCAGTGTTTCAAGCTCGACTACCTGTCTGGGTTCACTCAAGGCACCTTAGGttt                    <  3:364564/149‑1 (MQ=255)                                                                                                                                   gTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGtttt                   >  1:13467/1‑149 (MQ=255)                                                                                                                                    tttCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTg                  <  1:222339/149‑1 (MQ=255)                                                                                                                                     ttCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCt                                                           <  6:344284/107‑1 (MQ=255)                                                                                                                                       cGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGgt                <  7:272618/148‑1 (MQ=255)                                                                                                                                        gTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGgtgt              >  4:212493/1‑149 (MQ=255)                                                                                                                                        gTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGgtg               <  2:245132/148‑1 (MQ=255)                                                                                                                                         ttGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGtt             >  6:314102/1‑149 (MQ=255)                                                                                                                                         ttGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGAAGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGtt             >  1:94140/1‑149 (MQ=255)                                                                                                                                          tgagcgACAGCTTGCTGTC‑CTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCAc                             >  3:202921/6‑132 (MQ=255)                                                                                                                                          tgagcgACAGCTTGCTGTC‑CTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCAc                             <  4:202921/127‑1 (MQ=255)                                                                                                                                            agcgACAGCTTGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGa           >  8:189361/4‑149 (MQ=255)                                                                                                                                            aG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGAt          >  3:296932/1‑149 (MQ=255)                                                                                                                                             g‑gACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGATg         <  4:88661/149‑1 (MQ=255)                                                                                                                                                aCAGCAAGCTGTCGCTCAGCTCGCTCATCATGTACTTCAACGATGACAACAGCGAAAGCGGTATTGAACAGAAAGAAAGTTGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGTCACCGTAGGTTTTGGTGTTGATGtt       <  6:96635/149‑1 (MQ=255)                                                                                                                                                   gCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGATGTTCaa    <  3:194779/149‑1 (MQ=255)                                                                                                                                                     aaGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTTGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGATGTTCAAgc  <  4:372981/149‑1 (MQ=255)                                                                                                                                                     aaGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGATGTTCAAgc  <  3:212493/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    TGAGCGGGGCATCAGACCCCTCCGCCAGAGATACACAAAAATTAGAACGTAGAGGAGCAAAACATGTACAAGTCCAGCCTGGCTCTGGCCGTGGCACTGGGGGTTCTCGCCCAACAAGCAGGCGCTGCCGGTTTCGTTGAG‑GACAGCAAGCTGTCGCTCAGCTCGCGCACCATGTACTTCAACGATGACAACCGCGAAAGCGGTATTGACCAGAAAGAAAGTGGTCAGGGTTTCAAGCTCGACTACCTGTCTGGCTTCACTCAAGGCACCGTAGGTTTTGGTGTTGATGTTCAAGC  >  NC_002947/288519‑288814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑