Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,736,355 G→C 7.4% G1085R (GGC→CGC)  PP_2394 → leucine‑rich repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,736,3550GC 7.4% 231.8 / 3.0 75G1085R (GGC→CGC) PP_2394leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (36/33);  new base C (3/3);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTCCGGATGAGTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCGGGTTTCCCTACGG  >  NC_002947/2736207‑2736503
                                                                                                                                                    |                                                                                                                                                       
cGTTCCGGATGAGTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAg                                                                                                                                                         <  1:352760/149‑1 (MQ=255)
 gTTCCGGATGAGTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAgg                                                                                                                                                        >  7:149044/1‑149 (MQ=255)
 gTTCCGGATGAGTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACAAGATCACGCTCGACCAAgg                                                                                                                                                        >  7:204215/1‑149 (MQ=255)
       gATGAGTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAgga                                                                                                                                                  >  4:97461/1‑148 (MQ=255)
        aTGAGTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCg                                                                                                                                                 >  1:289948/1‑149 (MQ=255)
            gTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGTAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTg                                                                                                                                              <  1:244984/148‑1 (MQ=255)
            gTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTg                                                                                                                                              <  5:32729/148‑1 (MQ=255)
                cTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTcc                                                                                                                                          <  2:89162/148‑1 (MQ=255)
                 tGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTg                                                                                                                                        >  3:237747/1‑149 (MQ=255)
                   cGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTgg                                                                                                                                       <  1:156320/148‑1 (MQ=255)
                    gggCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTgg                                                                                                                                       >  7:14303/1‑147 (MQ=255)
                        gTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGAtt                                                                                                                                 <  2:2907/149‑1 (MQ=255)
                        gTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGAtt                                                                                                                                 >  2:319695/1‑149 (MQ=255)
                           tCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCATTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGc                                                                                                                               <  6:369268/148‑1 (MQ=255)
                            cccAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGa                                                                                                                                   <  6:334802/143‑1 (MQ=255)
                            cccAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGa                                                                                                                                   >  5:334802/1‑143 (MQ=255)
                            cccAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGcgg                                                                                                                             >  4:107614/1‑147 (MQ=255)
                            cccAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGCTAACCAGATCACGCTCGACCAAGGCGAGGCGTTGGTCCTGGCAGATTGCCg                                                                                                                             >  2:801/1‑149 (MQ=255)
                                aCTTGCGCAACCTGGAAATAACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTcc                                                                                                                         <  8:197989/149‑1 (MQ=255)
                                  ttGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGt                                                                                                                           >  8:271902/1‑145 (MQ=255)
                                  ttGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGt                                                                                                                           <  7:271902/145‑1 (MQ=255)
                                    gcgcAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCt                                                                                                                       <  6:33942/147‑1 (MQ=255)
                                     cgcAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTgg                                                                                                                     <  2:332265/148‑1 (MQ=255)
                                      gcAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGc                                                                                                                               <  2:317254/137‑1 (MQ=255)
                                      gcAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGc                                                                                                                               >  1:317254/1‑137 (MQ=255)
                                      gcAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAACGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGgtg                                                                                                                   >  6:20397/1‑149 (MQ=255)
                                       cAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGgtgt                                                                                                                  <  8:173913/149‑1 (MQ=255)
                                        aaCCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTa                                                                                                                 >  4:27603/1‑149 (MQ=255)
                                               aaaTCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑c                                                                                                         <  8:149044/149‑1 (MQ=255)
                                                    aCCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTAGCCCAGAAGCTGGAGGTACTCGACTAGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGATCGTCCTGCCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTATc                                                                                                  >  6:367326/1‑149 (MQ=255)
                                                     cccATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCg                                                                                                 >  3:75329/1‑149 (MQ=255)
                                                     cccATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCg                                                                                                 <  4:75329/149‑1 (MQ=255)
                                                     caaaTTGCCCACTCACGCGTTTGACATTGCCTTTGATCCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCAGGTAACCAGAAAACGCTCGACCAAGGCCAGGCCTTGGTCATGGCAGATAGCCGGTCCCTGGTTTAACTTAA‑A‑‑CTTACg                                                                                                 >  6:215868/4‑149 (MQ=255)
                                                           gCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACaa                                                                                            <  1:148227/148‑1 (MQ=255)
                                                           gCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACaa                                                                                            <  5:111946/148‑1 (MQ=255)
                                                           gCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCATTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACaa                                                                                            <  7:325238/148‑1 (MQ=255)
                                                            ccGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAAc                                                                                           <  4:237747/148‑1 (MQ=255)
                                                                       gTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGCA‑C‑‑CTTTCGGACAACCCGTTGCGCAgg                                                                               <  1:198163/149‑1 (MQ=255)
                                                                        tttGCGATTGCCTTTGCTGCTTGCCCAGAAGCTGGAGGTACTCGACTCGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAggg                                                                              <  6:111895/149‑1 (MQ=255)
                                                                        tttGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAggg                                                                              >  5:140387/1‑149 (MQ=255)
                                                                            ccATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCAtt                                                                          >  6:58831/1‑149 (MQ=255)
                                                                             cATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAGCCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCAttt                                                                         >  5:298468/1‑149 (MQ=255)
                                                                                 gCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCg                                                                     >  4:5704/1‑149 (MQ=255)
                                                                                   cTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGt                                                                    >  5:91499/1‑148 (MQ=255)
                                                                                    tttGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTc                                                                   >  8:166455/1‑148 (MQ=255)
                                                                                    tttGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCAGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTc                                                                   >  7:197703/1‑148 (MQ=255)
                                                                                      tgttGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTATCGTGATCACGCTCGACAAAGGCCTGGGGTTGGTCCTGGCAGATTGCCGGTGCCTGGTGTACCTGTA‑C‑‑CTTTCGGAGAACCCGTTGCGCAGGGCTTTTTCCGTcct                                                                 <  5:367326/145‑2 (MQ=255)
                                                                                        aTGCTTGCCCAGAAGCTGGAGTTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGc                                                              <  5:358744/149‑1 (MQ=255)
                                                                                           cTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCaa                                                            <  3:5704/148‑1 (MQ=255)
                                                                                            ttGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑CCACGATCGGACAACCAGTTGCGCAGGGCATTTTCGGTCCAGGaaa                                                            >  1:316354/1‑149 (MQ=255)
                                                                                            ttGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAAt                                                           >  2:349394/1‑148 (MQ=255)
                                                                                            ttGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATg                                                          >  5:212634/1‑149 (MQ=255)
                                                                                               cccAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGAc                                                        <  5:20397/148‑1 (MQ=255)
                                                                                                       cTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGaa                                               <  6:212634/149‑1 (MQ=255)
                                                                                                       cTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCACTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCTTACTGGCAATGACCGAACTGaa                                               >  8:323374/1‑149 (MQ=255)
                                                                                                          gAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCATGGCATTTTCCGTCCAGGCAATGACCGAACTGAAt                                              <  1:51190/147‑1 (MQ=255)
                                                                                                          gAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATg                                             <  3:27603/148‑1 (MQ=255)
                                                                                                          gAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAACGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAATC‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAAt                                              <  1:298422/148‑1 (MQ=255)
                                                                                                               aCTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTgg                                                                                                                                       >  1:256069/1‑56 (MQ=255)
                                                                                                               aCTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTgg                                                                                                                                       <  2:256069/56‑1 (MQ=255)
                                                                                                                cTCGACTTGTCCGGTAACCAGATCACGCTCGACCAACGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCctct                                       <  7:269719/148‑1 (MQ=255)
                                                                                                                    aCTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTg                                  >  7:204623/1‑149 (MQ=255)
                                                                                                                      ttGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGt                                 >  1:285119/1‑148 (MQ=255)
                                                                                                                             gTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACAcc                         <  3:107614/149‑1 (MQ=255)
                                                                                                                               aaCCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCGTCTACCTGTCCAACAcc                         >  6:103394/1‑147 (MQ=255)
                                                                                                                                 ccAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACAcc                         >  7:221668/1‑145 (MQ=255)
                                                                                                                                  cAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCGGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGAt                    >  1:265780/1‑149 (MQ=255)
                                                                                                                                  cAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGtt                                                                                      <  8:131823/83‑1 (MQ=255)
                                                                                                                                  cAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGtt                                                                                      >  7:131823/1‑83 (MQ=255)
                                                                                                                                     aTCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACAccc                        >  3:217484/1‑142 (MQ=255)
                                                                                                                                     aTCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACAccc                        <  4:217484/142‑1 (MQ=255)
                                                                                                                                        aCGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCg               <  5:58831/148‑1 (MQ=255)
                                                                                                                                          gCTCGACCAAGTCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCGGGt            >  3:362786/1‑149 (MQ=255)
                                                                                                                                          gCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCGGGt            >  1:285345/1‑149 (MQ=255)
                                                                                                                                          ccaggACCAACGCCTGGCCTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCGGGt            >  6:92868/5‑149 (MQ=255)
                                                                                                                                           caggACCAACGCCTGGCCTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTc                                                                   >  6:47437/4‑93 (MQ=39)
                                                                                                                                           caggACCAACGCCTGGCCTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTc                                                                   <  5:47437/90‑1 (MQ=39)
                                                                                                                                                   aGGCCAGGCGTTGGTCCTCGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGATGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCGGGTTTCCCTACg   >  6:17014/1‑149 (MQ=255)
                                                                                                                                                    ggCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCGGGTTTCCCTACgg  <  6:91499/149‑1 (MQ=255)
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CGTTCCGGATGAGTTTCTGCGGGCGTTTCCCAACTTGCGCAACCTGGAAATCACCCATTGCCGACTCACGCGTTTGCCATTGCCTTTGATGCTTGCCCAGAAGCTGGAGGTACTCGACTTGTCCGGTAACCAGATCACGCTCGACCAAGGCCAGGCGTTGGTCCTGGCAGATTGCCGGTCCCTGGTGTACCTGAA‑C‑‑CTTTCGGACAACCCGTTGCGCAGGGCATTTTCCGTCCAGGCAATGACCGAACTGAATGCCCTCTACCTGTCAAACACCCAGATGCCCGGGTTTCCCTACGG  >  NC_002947/2736207‑2736503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑