Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,749,956 C→G 6.1% E178D (GAG→GAC) ‡ PP_2406 ← quinate/shikimate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,749,9560CG 6.1% 211.4 / 3.8 65E178D (GAG→GAC) ‡PP_2406quinate/shikimate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (31/30);  new base G (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

TCTCTGCCACCCACAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGAGCCAGCGCCACCGGCACCCATCTGCACCACC  >  NC_002947/2749814‑2750103
                                                                                                                                              |                                                                                                                                                   
tctcTGCCACCCACAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCcgcg                                                                                                                                               >  6:190882/1‑149 (MQ=255)
 ctctGCCACCCACAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCgcgc                                                                                                                                              >  5:162667/1‑149 (MQ=255)
  tctGCCACCCACAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCCCCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGcc                                                                                                                                             >  6:313571/1‑149 (MQ=255)
    tGCCACCCACAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCg                                                                                                                                            >  7:180938/1‑148 (MQ=255)
         cccacAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATgg                                                                                                                                      <  2:109372/149‑1 (MQ=255)
         cccacAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATgg                                                                                                                                      <  4:148310/149‑1 (MQ=255)
          ccacAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGGAGCGCGCCgtgcttgg                                                                                                                                     <  8:972/149‑8 (MQ=255)
           cacaGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGGTGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGgt                                                                                                                                    <  5:336308/149‑1 (MQ=255)
            acaGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGgtg                                                                                                                                   >  6:148525/1‑149 (MQ=255)
            acaGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGgtg                                                                                                                                   >  2:468/1‑149 (MQ=255)
              aGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGtt                                                                                                                                 >  7:15029/1‑149 (MQ=255)
                   gggATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGt                                                                                                                            >  5:74306/1‑149 (MQ=255)
                    ggATGCAACAACTCCACCTTCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGtt                                                                                                                           <  2:155372/149‑1 (MQ=255)
                       tGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGt                                                                                                                         <  8:15029/148‑1 (MQ=255)
                        gCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCACCGAAATGGGTGtt                                                                                                                                 <  1:160810/139‑1 (MQ=255)
                        gCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCACCGAAATGGGTGtt                                                                                                                                 >  2:160810/1‑139 (MQ=255)
                         caacaaCTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTcc                                                                                                                       <  6:384534/148‑1 (MQ=255)
                          aacaacTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATgg                                                                                                                                      <  5:377290/132‑1 (MQ=255)
                          aacaacTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATgg                                                                                                                                      >  8:288685/1‑132 (MQ=255)
                          aacaacTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATgg                                                                                                                                      >  6:377290/1‑132 (MQ=255)
                          aacaacTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATgg                                                                                                                                      <  7:288685/132‑1 (MQ=255)
                          aacaacTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTccac                                                                                                                     >  5:311262/1‑149 (MQ=255)
                                tCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGc                                                                                                               >  6:19415/1‑149 (MQ=255)
                                  cACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCACCGCCt                                                                                                             >  5:111242/1‑149 (MQ=255)
                                  cACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGc                                                                                                               <  1:468/147‑1 (MQ=255)
                                  cACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCt                                                                                                             <  8:180938/149‑1 (MQ=255)
                                          gTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACgg                                                                                                     <  5:190882/149‑1 (MQ=255)
                                             gCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTc                                                                                                  <  5:313571/149‑1 (MQ=255)
                                                   ccGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATcc                                                                                            <  8:4361/149‑1 (MQ=255)
                                                    cGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCa                                                                                           >  5:152803/1‑149 (MQ=255)
                                                       tAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACtt                                                                                        <  3:177609/149‑1 (MQ=255)
                                                          cTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCg                                                                                      >  7:45035/1‑148 (MQ=255)
                                                             ggCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGaa                                                                                    <  4:283768/147‑1 (MQ=255)
                                                                  tGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCAcc                                                                             <  6:162667/149‑1 (MQ=255)
                                                                     ccACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGc                                                                                                               <  7:141599/112‑1 (MQ=255)
                                                                     ccACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGc                                                                                                               >  8:141599/1‑112 (MQ=255)
                                                                      cACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGcc                                                                         <  6:311262/149‑1 (MQ=255)
                                                                      cACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGcc                                                                         >  4:137676/1‑149 (MQ=255)
                                                                         cGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGtt                                                                      <  5:148525/149‑1 (MQ=255)
                                                                          ggCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCCCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTc                                                                     <  2:340337/149‑1 (MQ=255)
                                                                             gtggtgTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTAc                                                                  <  6:74306/149‑1 (MQ=255)
                                                                             gtggtgTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTAc                                                                  <  5:110677/149‑1 (MQ=255)
                                                                                  gtTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCtt                                                             >  7:260092/1‑149 (MQ=255)
                                                                                   ttGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTc                                                            >  8:271871/1‑149 (MQ=255)
                                                                                     gACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCa                                                           <  8:45035/148‑1 (MQ=255)
                                                                                      aCCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGc                                                                                                >  6:82548/1‑110 (MQ=255)
                                                                                      aCCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGc                                                                                                <  5:82548/110‑1 (MQ=255)
                                                                                       ccAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCAccc                                                        <  5:19415/149‑1 (MQ=255)
                                                                                        cAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCAGCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCca                                                       >  2:190901/1‑149 (MQ=255)
                                                                                           cccGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCcagca                                                    <  8:260092/149‑1 (MQ=255)
                                                                                                   cTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCCCGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATgg                                            >  7:320113/1‑149 (MQ=255)
                                                                                                        gCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCAc                                                          >  7:242649/1‑130 (MQ=255)
                                                                                                        gCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCAc                                                          <  8:242649/130‑1 (MQ=255)
                                                                                                          cAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACgg                                     >  1:294128/1‑149 (MQ=255)
                                                                                                              gCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGcc                                   >  2:96275/1‑147 (MQ=255)
                                                                                                                  tgGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGAGc                               >  7:274129/1‑147 (MQ=255)
                                                                                                                      cAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATgg                                            >  4:191744/1‑130 (MQ=255)
                                                                                                                      cAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATgg                                            <  3:191744/130‑1 (MQ=255)
                                                                                                                                 ccatttcGGCGCGGAGCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGAGCCAGCGCCACCGGCAcc               >  8:211201/7‑148 (MQ=255)
                                                                                                                                  aaGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAgg                                                  <  2:286953/112‑1 (MQ=255)
                                                                                                                                  aaGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAgg                                                  >  1:286953/1‑112 (MQ=255)
                                                                                                                                    tttcGGCGCGGAGCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGa                                 <  4:95907/124‑1 (MQ=255)
                                                                                                                                    tttcGGCGCGGAGCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGa                                 >  3:95907/4‑127 (MQ=255)
                                                                                                                                     cACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACAAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGAGCCAGCGCCACCGGCACCCATCt          >  4:287889/1‑149 (MQ=255)
                                                                                                                                        ggCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGAGCCAGCGCCACCGGCACCCATCTGca       >  6:70692/1‑149 (MQ=255)
                                                                                                                                             gcTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGAGCCAGCGCCACCGGCACCCATCTGCaccacc  <  1:96275/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
TCTCTGCCACCCACAGGCGGGGATGCAACAACTCCACCGGCAGTGGCGTGCCCGGTAGCTTGGCCATGCCCACCGGCGTGGTGTTGACCAGCCCGTCCGCTTCGGCCAAGGCTGTGGCCAGGTCGGTGCCAAGCACGGCGCGCTCCGCGCCGAAATGGGTGTTCAGGTTGTCCACCAACGCCTGCGCACGGGTCGCATCCACTTCGAACAGCACCAGCCGTTCTACCCCTTCACCCAGCAGGGCATGGGCCACGGCCGAGCCAGCGCCACCGGCACCCATCTGCACCACC  >  NC_002947/2749814‑2750103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑