Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,796,838	A→T	10.2%	intergenic (+22/‑110)	PP_t43 → / → PP_2449	tRNA‑Val/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,796,838	0	A	T	10.2%	161.7 / 7.9	59	intergenic (+22/‑110)	PP_t43/PP_2449	tRNA‑Val/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/29);  new base T (3/3);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

CTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAGTGCTCGCCCT  >  NC_002947/2796696‑2796985                                                                                                                                               |                                                                                                                                                     cTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATc                                                                                                                                                <  4:23684/149‑1 (MQ=255)  tttACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCg                                                                                                                                               >  7:251808/1‑149 (MQ=255)     aCAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCATTTCTGATCGcc                                                                                                                                             <  6:133456/148‑1 (MQ=255)       aGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCtt                                                                                                                                          >  6:317364/1‑149 (MQ=255)         gTACGCATCGGGCCACTAATATGCGCCCCACACGACAGGCACATAGCTCAGTTGGATAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGTCGATCAGAAATGATCGCCCtttt                                                                                                                                        >  5:266476/1‑149 (MQ=255)          tACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCttttt                                                                                                                                       <  1:95531/149‑1 (MQ=255)               aTCTGGCCACTAATATGCGCACAACACGACAGGCACATAGCTCAGATGGTTAGAGCACCACCATGAAATGGTGGGGGTCGTTGGTTCGAGTCAAATTGTGCCTACCAAATCGAAAAAAGGCGATCAGAAATTATAGAACTTTTTGA‑Att                                                                                                                                 >  8:114235/1‑149 (MQ=17)                  gggCCACTAATATGCTCACCACACGACAGGCACATAGCTCAGTTGGTTAGTGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTgc                                                                                                                              <  8:296918/149‑1 (MQ=255)                      cACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATGCAAAAAGGGCGATCATTTCTGATCGCCCTTTTTCG‑ATTTggtagg                                                                                                                          <  5:97888/149‑6 (MQ=14)                          ccgatGCGTACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAag                                                                                                                      <  5:324204/146‑1 (MQ=255)                                   accacACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCgtgt                                                                                                              >  5:129889/1‑148 (MQ=255)                                      acacGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACg                                                                                                          <  1:161947/149‑1 (MQ=255)                                          gACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTg                                                                                                      <  1:158517/149‑1 (MQ=255)                                          gACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTg                                                                                                      >  2:250509/1‑149 (MQ=255)                                           aCAGTCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTgg                                                                                                     <  4:213714/149‑1 (MQ=255)                                           aCAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTgg                                                                                                     <  3:72195/149‑1 (MQ=255)                                                   aTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATAAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTc                                                                                             >  8:355219/1‑149 (MQ=255)                                                   aTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGCTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCATTTCTGATCGCCCt                                                                                                                                           <  1:169600/104‑1 (MQ=14)                                                   aTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGCTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCATTTCTGATCGCCCt                                                                                                                                           >  2:169600/1‑104 (MQ=14)                                                     aGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCg                                                                                                                         <  3:372338/119‑1 (MQ=255)                                                     aGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCg                                                                                                                         >  4:372338/1‑119 (MQ=255)                                                       cTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCggg                                                                                         <  8:2419/149‑1 (MQ=255)                                                          aGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGc                                                                                        <  6:45436/147‑1 (MQ=255)                                                           gTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGCTCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGACGCGGCGTGTATCGTGTGGTCGCGGTCCGGGCaga                                                                                     >  4:160413/1‑147 (MQ=21)                                                                  agagCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGc                                                                              >  1:86916/1‑149 (MQ=255)                                                                           ccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGaa                                                                     >  7:90731/1‑149 (MQ=255)                                                                               gACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATc                                                                   <  1:250509/147‑1 (MQ=255)                                                                                aCATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCg                                                                 <  5:317364/148‑1 (MQ=255)                                                                                aCATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGc                                                                <  6:224221/149‑1 (MQ=255)                                                                                 cATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGcc                                                               <  7:355219/149‑1 (MQ=255)                                                                                     gtggGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCtt                                                           >  5:371825/1‑149 (MQ=255)                                                                                        ggggTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTa                                                                        >  2:359167/1‑133 (MQ=255)                                                                                        ggggTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTa                                                                        <  1:359167/133‑1 (MQ=255)                                                                                              gTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTg                                                   <  1:150661/148‑1 (MQ=255)                                                                                                 ggTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCGTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACTTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCTAATCTGGAg                                                <  5:4126/148‑1 (MQ=255)                                                                                                  gTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGccc                                                                                                                                            >  6:73206/1‑56 (MQ=255)                                                                                                  gTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGccc                                                                                                                                            <  5:73206/56‑1 (MQ=255)                                                                                                         tCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACAcgcg                                       >  4:62/1‑149 (MQ=255)                                                                                                           cAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTg                                     >  1:63843/1‑149 (MQ=255)                                                                                                              ttGTGCCTACCAAATCGAAAAAGGTCGATCAGAAATGCTCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTgcgc                                  <  6:266476/149‑1 (MQ=255)                                                                                                                    cTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAg                                                <  6:348857/129‑1 (MQ=255)                                                                                                                    cTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAg                                                >  5:348857/1‑129 (MQ=255)                                                                                                                     tACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCg                                                                                           <  8:137507/85‑1 (MQ=255)                                                                                                                     tACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCg                                                                                           >  7:137507/1‑85 (MQ=255)                                                                                                                     tACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTgg                           >  7:117034/1‑149 (MQ=255)                                                                                                                      aCCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTcgcg                                                                                                <  5:51323/79‑1 (MQ=255)                                                                                                                      aCCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTcgcg                                                                                                >  6:51323/1‑79 (MQ=255)                                                                                                                       ccAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTgg                           <  4:169906/147‑1 (MQ=255)                                                                                                                       ccAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGc                          <  2:86916/148‑1 (MQ=255)                                                                                                                               aaaaaGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAAt                 <  6:371825/149‑1 (MQ=255)                                                                                                                                aaaaGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCTGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATg                <  8:251808/149‑1 (MQ=255)                                                                                                                                   aGGGCGATCATTTCTGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCa             >  4:206070/1‑149 (MQ=255)                                                                                                                                   aGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCa             <  6:129889/149‑1 (MQ=255)                                                                                                                                    gggCGATCATTTCTGATCGCCCTTTTTGCGATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATgg               >  1:28537/1‑147 (MQ=255)                                                                                                                                    gggCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAg            >  6:245362/1‑149 (MQ=255)                                                                                                                                       cGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAGTGc         >  6:256182/1‑149 (MQ=255)                                                                                                                                         aTCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCa             >  4:344937/1‑143 (MQ=255)                                                                                                                                         aTCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCa             <  3:344937/143‑1 (MQ=255)                                                                                                                                          tCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAGTGCTCg      >  5:223883/1‑149 (MQ=255)                                                                                                                                              aaaTGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAGACTGGCTGTGGTAATGGCAGTGCTCGCCCt  >  5:203181/1‑149 (MQ=255)                                                                                                                                              aaaTGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAGTGCTCGCCCt  >  7:148488/1‑149 (MQ=255)                                                                                                                                              aaaTGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCACTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAGTGCTCGCCCt  >  5:359294/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                     CTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGC‑ATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAGTGCTCGCCCT  >  NC_002947/2796696‑2796985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑