Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,801,973 C→A 7.3% R119R (CGC→CGA)  rbsA‑I → ribose ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,801,9730CA 7.3% 174.2 / 2.9 55R119R (CGC→CGA) rbsA‑Iribose ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGATCG  >  NC_002947/2801832‑2802121
                                                                                                                                             |                                                                                                                                                    
gACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCt                                                                                                                                               >  2:182007/1‑149 (MQ=255)
 aCCTACCGCTGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCTCATGGTCATGCAGTAGCTGAACCTGCTGCCGACTCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTg                                                                                                                                              <  2:105911/149‑1 (MQ=255)
 aCCTACCGCGGCCAGGCCTACGCGCACGGCAGCCGGGGCGAGGCCGATCTCCTTGGGGTGCTCATGGGCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTg                                                                                                                                              <  2:100935/149‑1 (MQ=255)
   cTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGa                                                                                                                                            >  5:325031/1‑149 (MQ=255)
      ccGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCtt                                                                                                                                                    >  5:373129/1‑138 (MQ=255)
      ccGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCtt                                                                                                                                                    <  6:373129/138‑1 (MQ=255)
       cgcgGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAg                                                                                                                                        <  1:305587/149‑1 (MQ=255)
           gCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCcac                                                                                                                                    <  4:336506/149‑1 (MQ=255)
                   tACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCTTGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCAACAAGCGttt                                                                                                                            >  4:209738/1‑149 (MQ=255)
                      gcgcCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCa                                                                                                                                         >  8:143918/1‑133 (MQ=255)
                      gcgcCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCa                                                                                                                                         <  7:143918/133‑1 (MQ=255)
                       cgcCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTgcgc                                                                                                                        <  1:182007/149‑1 (MQ=255)
                          ccGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACaa                                                                                                                                  <  4:209023/136‑1 (MQ=255)
                          ccGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACaa                                                                                                                                  >  3:209023/1‑136 (MQ=255)
                                        gAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGc                                                                                                       >  2:225628/1‑149 (MQ=255)
                                             cGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGTTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATgg                                                                                                  >  6:93825/1‑149 (MQ=255)
                                              gAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGc                                                                                                 >  8:313676/1‑149 (MQ=255)
                                                    cTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGAt                                                                                                                                           <  2:64859/101‑1 (MQ=255)
                                                    cTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGAt                                                                                                                                           >  1:64859/1‑101 (MQ=255)
                                                    cTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCtcgggt                                                                                           >  3:329542/1‑149 (MQ=255)
                                                       ggggTTGGCATGTTCATGCAGTAGCTGAACGTGCTGCCGACGCTGAGGGGGGCGGAAAACCTGTTTCTCGACAACCTGGCCAGCCGCTTCGGCTGGATCAGCGACAAGCGTTTGCGGCAGCTGGCAACGGCTGCCATGGCtcgggtcg                                                                                         <  4:259178/148‑1 (MQ=255)
                                                        gggTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCtcgggtcggg                                                                                       >  7:38444/1‑149 (MQ=255)
                                                         ggTGCGAATGGTCATGCAGGATCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGc                                                                                      <  3:306666/149‑1 (MQ=255)
                                                            gcgcATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCtcgggtcg                                                                                         >  4:327186/1‑143 (MQ=255)
                                                            gcgcATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCtcgggtcg                                                                                         <  3:327186/143‑1 (MQ=255)
                                                            gcgcATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTc                                                                                    >  1:100716/1‑148 (MQ=255)
                                                               cATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACg                                                                                >  3:40115/1‑149 (MQ=255)
                                                                    tCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCAtt                                                                           <  8:38444/149‑1 (MQ=255)
                                                                         cAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGAccc                                                                      <  2:100716/149‑1 (MQ=255)
                                                                               cTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGacac                                                                >  3:304345/1‑149 (MQ=255)
                                                                               cTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGacac                                                                >  5:354713/1‑149 (MQ=255)
                                                                                      tgctgcCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCtcggg                                                                                            <  3:251753/114‑1 (MQ=255)
                                                                                      tgctgcCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCtcggg                                                                                            >  4:251753/1‑114 (MQ=255)
                                                                                        ttgcCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTc                                                         <  5:93825/146‑1 (MQ=255)
                                                                                         tgcCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGc                                                      >  3:362884/1‑149 (MQ=255)
                                                                                             gACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCa                                                                             <  5:303336/122‑1 (MQ=255)
                                                                                             gACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCa                                                                             >  6:303336/1‑122 (MQ=255)
                                                                                              aCGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACt                                                 >  4:132895/1‑149 (MQ=255)
                                                                                                gCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTgg                                               <  6:354713/149‑1 (MQ=255)
                                                                                                gCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTgg                                               <  6:325031/149‑1 (MQ=255)
                                                                                                            ggAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCAt                                    >  6:192729/1‑148 (MQ=255)
                                                                                                            ggAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATc                                   <  4:40115/149‑1 (MQ=255)
                                                                                                             gAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCa                                  >  6:203283/1‑149 (MQ=255)
                                                                                                             gAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCa                                  <  7:28825/149‑1 (MQ=255)
                                                                                                                aaCCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGAcc                                                                       >  3:170996/1‑109 (MQ=255)
                                                                                                                aaCCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGAcc                                                                       <  4:170996/109‑1 (MQ=255)
                                                                                                                      tttCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATg                           <  8:345043/147‑1 (MQ=255)
                                                                                                                           cGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCgaga                    <  4:304345/149‑1 (MQ=255)
                                                                                                                            gACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCgaga                    <  6:319142/148‑1 (MQ=255)
                                                                                                                                 ccTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGccc              <  1:199868/149‑1 (MQ=255)
                                                                                                                                    atccAGCCGAAGCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCga                      >  4:342441/3‑138 (MQ=255)
                                                                                                                                    atccAGCCGAAGCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCga                      <  3:342441/136‑1 (MQ=255)
                                                                                                                                     tccAGCCGAAGCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCaa          >  5:370696/2‑149 (MQ=255)
                                                                                                                                     tccAGCCGAAGCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCaa          <  6:370696/148‑1 (MQ=255)
                                                                                                                                      ccAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAAc         >  4:156254/1‑149 (MQ=255)
                                                                                                                                             cTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGATCg  <  3:209738/149‑1 (MQ=255)
                                                                                                                                             cTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGATCg  <  7:313676/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGATCG  >  NC_002947/2801832‑2802121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑