Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,884,813 C→G 12.4% P93R (CCG→CGG) ‡ PP_2540 → short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,884,8130CG12.4% 124.8 / 8.5 47P93R (CCG→CGG) ‡PP_2540short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base G (2/4);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGGGTGGTGAATATCACCTCCGG  >  NC_002947/2884666‑2884960
                                                                                                                                                   |                                                                                                                                                   
tGGAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCg                                                                                                                                                    >  1:10330/1‑149 (MQ=255)
  gAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGcc                                                                                                                                                  >  7:343610/1‑149 (MQ=255)
       gACCGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGGGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCGGGCCACCgg                                                                                                                                             <  2:89998/149‑1 (MQ=255)
        aCCGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGTGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCggg                                                                                                                                            <  3:196431/149‑1 (MQ=255)
         ccGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCgggg                                                                                                                                           <  6:139552/149‑1 (MQ=255)
         ccGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCgggg                                                                                                                                           <  2:379844/149‑1 (MQ=255)
          cGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCGGGCCACCggcg                                                                                                                                           >  6:89735/1‑146 (MQ=255)
          cGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCGGGCCACCggcg                                                                                                                                           <  5:89735/148‑3 (MQ=255)
               gCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTc                                                                                                                                     >  2:232216/1‑149 (MQ=255)
                   gTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTg                                                                                                                                 <  6:309832/149‑1 (MQ=255)
                    ttGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGa                                                                                                                                >  8:205978/1‑149 (MQ=255)
                        gcAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTgg                                                                                                                            <  7:205978/149‑1 (MQ=255)
                           gggCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTggggg                                                                                                                         <  4:277224/149‑1 (MQ=255)
                            ggCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGc                                                                                                                        >  8:211532/1‑149 (MQ=255)
                                    cGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGAc                                                                                                                <  7:355521/149‑1 (MQ=255)
                                       gAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTgg                                                                                                             <  5:34764/149‑1 (MQ=255)
                                             aGGTCCATACCGTGACAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAg                                                                                                       <  3:66230/149‑1 (MQ=255)
                                                   aTACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTg                                                                                                 >  1:104654/1‑149 (MQ=255)
                                                        gTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGc                                                                                            <  1:158387/149‑1 (MQ=255)
                                                           gCAGGCGATGTTGCCGTTGCGAAGGTGCGGCAGGCGCTTTTGGCGGCCTGCCCTCAGGTCTACATTCTGGTCAACAACGCCGGTGGCCGGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGGCa                                                                                          <  2:10330/148‑1 (MQ=255)
                                                           gCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCgggg                                                                                                                                           >  8:157060/1‑99 (MQ=255)
                                                           gCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCgggg                                                                                                                                           <  7:157060/99‑1 (MQ=255)
                                                            cAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAAc                                                                                        <  8:231481/149‑1 (MQ=255)
                                                                       gCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTg                                                                                                              >  8:245703/1‑116 (MQ=255)
                                                                       gCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTg                                                                                                              <  7:245703/116‑1 (MQ=255)
                                                                               cAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGc                                                                     >  7:48252/1‑149 (MQ=255)
                                                                               cAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGc                                                                     >  3:124986/1‑149 (MQ=255)
                                                                                aaGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCt                                                                    >  6:37512/1‑149 (MQ=255)
                                                                                  ggTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGa                                                                  <  4:124986/149‑1 (MQ=255)
                                                                                      cgcCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCaa                                                              <  2:377968/149‑1 (MQ=255)
                                                                                            gcgcTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCggt                                                        <  2:104654/149‑1 (MQ=255)
                                                                                             cgcTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCggtg                                                       >  5:131949/1‑149 (MQ=255)
                                                                                               cTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCggtggt                                                     <  1:232216/149‑1 (MQ=255)
                                                                                                               cGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAg                                     >  4:136805/1‑149 (MQ=255)
                                                                                                                   ggTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTg                                 <  8:343610/149‑1 (MQ=255)
                                                                                                                    gTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTgg                                >  1:364319/1‑149 (MQ=255)
                                                                                                                       gACATTCTGGTCAACAACGCCGGTGGCCCGCCTCCGGGTGACTTCCGTGATTGGGGTCGTGATGACTGGCTGAAGGCTCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGtt                              <  5:252475/148‑1 (MQ=255)
                                                                                                                        aCATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGtttt                            >  7:16918/1‑149 (MQ=255)
                                                                                                                        aCATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGtttt                            <  5:350118/149‑1 (MQ=255)
                                                                                                                        aCATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGtttt                            <  3:136805/149‑1 (MQ=255)
                                                                                                                        aCATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGACGAGCGGGGtttt                            >  3:25625/1‑149 (MQ=255)
                                                                                                                              tGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGtttt                            <  6:374457/143‑1 (MQ=255)
                                                                                                                              tGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGtttt                            >  5:374457/1‑143 (MQ=255)
                                                                                                                                   aacaacGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGggtggt                 >  4:169701/1‑149 (MQ=255)
                                                                                                                                     caacGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGGGTGGTGa               <  3:169701/149‑1 (MQ=255)
                                                                                                                                          ccGGTGGCGGGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGGGTGGTGAatat           >  4:186458/1‑148 (MQ=255)
                                                                                                                                          ccGGTGGCGGGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGGGTGGTGAATATc          <  3:186458/149‑1 (MQ=255)
                                                                                                                                                 cccGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCCCGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGGGTGGTGAATATCACCTCCg   >  7:327831/1‑149 (MQ=255)
                                                                                                                                                  ccGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGGGTGGTGAATATCACCTCCgg  >  5:24628/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGCCCGGGAATCGAGGTCCATACCGTGGCAGGCGATGTTGCCGATCCCAAGGTGCGCCAGGCGCTGTTGGCGGCCTGCCCGCAGGTCGACATTCTGGTCAACAACGCCGGTGGCCCGCCACCGGGGGACTTCCGTGATTGGGGGCGTGATGACTGGCTGAAGGCGCTGGACGCCAACATGCTCACGCCCATCGAGCTGATCAAGGCGGTGGTAGATGGCATGGCCGAGCGTGGTTTTGGCCGGGTGGTGAATATCACCTCCGG  >  NC_002947/2884666‑2884960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑