Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,552 G→A 7.7% L373L (CTG→TTG)  PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5520GA 7.7% 150.1 / 2.2 52L373L (CTG→TTG) PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base A (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

ATCGTCCAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCG  >  NC_002947/2887406‑2887699
                                                                                                                                                   |                                                                                                                                                   
aTCGTCCAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGgc                                                                                                                                                   >  8:289294/1‑149 (MQ=255)
   gTCCAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGgcgc                                                                                                                                                 <  6:250686/148‑1 (MQ=255)
     ccAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAAgcacctc                                                                                                                                              <  5:262773/149‑6 (MQ=255)
       aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                            <  4:108774/149‑1 (MQ=255)
          aaCGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTg                                                                                                                                         >  1:337732/1‑149 (MQ=255)
               ggCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTgc                                                                                                                                    <  7:289294/149‑1 (MQ=255)
               ggCACTGTCGGGAATCGCCGGATGGCGGGGCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTgc                                                                                                                                    <  7:255202/149‑1 (MQ=255)
                gCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTAATCCGATTCGGTGAACAATTCACAGCCCAGTCGATGCAGATGACGGCCAACTT‑CCTGATGCGCCTAGGCCAGGCGCTGGCGCCGGCGCTTGAGgtgcgtgc                                                                                                                                    >  8:255202/1‑148 (MQ=255)
                 cACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTgcgc                                                                                                                                  <  1:152373/149‑1 (MQ=255)
                   cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCa                                                                                                                                <  3:309303/149‑1 (MQ=255)
                      tCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                              <  3:320413/148‑1 (MQ=255)
                      tCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAgc                                                                                                                              <  6:22775/148‑1 (MQ=39)
                        gggAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGcc                                                                                                                           >  2:24046/1‑149 (MQ=255)
                         ggAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAACGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGccc                                                                                                                          <  3:68102/149‑1 (MQ=255)
                             tCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTc                                                                                                                       >  8:292281/1‑148 (MQ=255)
                               gCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                            >  2:9661/1‑125 (MQ=255)
                               gCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCgat                                                                                                                    >  1:249713/1‑149 (MQ=255)
                               gCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCCGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                            <  1:9661/125‑1 (MQ=255)
                                   gATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATc                                                                                                                <  1:61627/149‑1 (MQ=255)
                                      ggggggTCCATTTGTACATGCCTTCATCCTATTCGGTTAACAATTCAAAGCCCAGTCTATGCAGATGCCGCCCAACTT‑CCTCAGGCGCCGCGGCCAGGCGCTGGCGCAGGCGCTTGTGGTGCTTGCGCCAGGGCCCGTCGATGATcgc                                                                                                              <  2:282806/145‑1 (MQ=255)
                                            tCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATac                                                                                                       <  1:239486/149‑1 (MQ=255)
                                             ccAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATaca                                                                                                      >  2:74872/1‑149 (MQ=255)
                                             ccAGATGTACAAGCCTTCATCCGATACGGTCAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACAT‑CCTGATGCGCCTCCGCCAGGCGCAGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGAACGCCGCATaca                                                                                                      >  8:80340/1‑149 (MQ=255)
                                                      cATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                              <  7:292281/148‑1 (MQ=255)
                                                       aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTc                                                                                            >  5:176608/1‑149 (MQ=255)
                                                                       cGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAggt                                                                             <  6:176608/148‑1 (MQ=255)
                                                                         gTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTGCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAggtgg                                                                           >  1:262537/1‑149 (MQ=255)
                                                                          tGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCAAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACATCACGCGCTCCATCACCTGCGAGGTGGTc                                                                         <  1:24046/149‑1 (MQ=255)
                                                                            aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                              <  7:15105/126‑1 (MQ=255)
                                                                            aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                              >  8:15105/1‑126 (MQ=255)
                                                                             aaaaTTCAAAGCCCCGTCGATGCTGATGCCGGCAAACTT‑CCTTTTGCGCCTCGGCCAGGCGCTGGCGCTGGCGTTTGTGGGGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAgg                                                                      <  2:337732/147‑1 (MQ=255)
                                                                                 ttCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCt                                                                   >  4:269330/1‑148 (MQ=255)
                                                                                          cAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAgc                                                               >  1:165404/1‑143 (MQ=255)
                                                                                          cAGTCGATGCAGATGCCGGCCAACTT‑CCTGAGGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCt                                                         <  2:249713/149‑1 (MQ=255)
                                                                                             tCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCa                                                      <  1:74872/149‑1 (MQ=255)
                                                                                               gATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAgg                                                    >  8:242406/1‑149 (MQ=255)
                                                                                                          cGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTc                                                       <  4:106503/135‑1 (MQ=255)
                                                                                                          cGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTc                                                       >  3:106503/1‑135 (MQ=255)
                                                                                                              cAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTg                                      >  5:81100/1‑148 (MQ=255)
                                                                                                              cAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGc                                     <  6:81100/149‑1 (MQ=255)
                                                                                                                  tt‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCa                                 <  3:269330/149‑1 (MQ=255)
                                                                                                                      cTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGtt                              <  2:262537/149‑1 (MQ=255)
                                                                                                                       tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                             >  1:52474/1‑149 (MQ=255)
                                                                                                                         aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCgg                           >  7:28513/1‑149 (MQ=255)
                                                                                                                          tGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGCGGTGCTGGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCggg                          <  2:93851/149‑1 (MQ=255)
                                                                                                                                  ggCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGc                    >  7:24817/1‑147 (MQ=255)
                                                                                                                                   gCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAg                 >  2:326333/1‑149 (MQ=255)
                                                                                                                                        gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATg             >  5:308677/1‑148 (MQ=38)
                                                                                                                                         cgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATg             >  5:252861/1‑147 (MQ=38)
                                                                                                                                          gcTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCTTCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAg          <  7:242406/149‑1 (MQ=255)
                                                                                                                                            tGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGcc        >  7:488/1‑149 (MQ=255)
                                                                                                                                            tGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGcc        >  7:122594/1‑149 (MQ=255)
                                                                                                                                                   ggCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCAATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCg  >  1:63572/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCGTCCAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTT‑CCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCG  >  NC_002947/2887406‑2887699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑