Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,951,334 G→C 13.0% intergenic (‑113/+48) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,951,3340GC13.0% 133.8 / 13.2 54intergenic (‑113/+48)oguA/PP_25858‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base C (5/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.18e-02

AGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTT  >  NC_002947/2951193‑2951480
                                                                                                                                             |                                                                                                                                                  
agCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTa                                                                                                                                             <  6:175834/149‑1 (MQ=255)
 gCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTggcggc                                                                                                                                               >  4:344258/1‑146 (MQ=255)
 gCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCtct                                                                                                                                            <  3:125350/149‑3 (MQ=255)
  cGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTaga                                                                                                                                           <  3:9311/149‑1 (MQ=255)
   gTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTagag                                                                                                                                          >  3:371263/1‑149 (MQ=255)
    tttttGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGc                                                                                                                                         >  1:332377/1‑149 (MQ=255)
    tttttGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGc                                                                                                                                         >  6:312889/1‑149 (MQ=255)
          cAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCcgcg                                                                                                                                     >  7:6718/1‑147 (MQ=255)
             ggTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAagag                                                                                                                                <  8:101135/149‑1 (MQ=255)
                 tGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGcc                                                                                                                             <  8:6718/148‑1 (MQ=255)
                  gTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCg                                                                                                                            <  5:152593/148‑1 (MQ=255)
                                gCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCcgcc                                                                                                                                     >  8:210121/1‑124 (MQ=255)
                                gCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCcgcc                                                                                                                                     <  7:210121/125‑2 (MQ=255)
                                 cAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCa                                                                                                            >  2:246237/1‑149 (MQ=255)
                                      cTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGaaa                                                                                                       <  3:112912/149‑1 (MQ=255)
                                      cTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCCCGAAGAGGCCGGCACGGGTTGCAGCCATGa                                                                                                         >  8:223942/1‑147 (MQ=255)
                                        gcgcAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAg                                                                                                      >  1:364547/1‑148 (MQ=255)
                                                cGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACAcc                                                                                              <  2:332377/148‑1 (MQ=255)
                                                    tCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTc                                                                                         >  1:256512/1‑149 (MQ=255)
                                                    tCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTc                                                                                         >  4:10224/1‑149 (MQ=255)
                                                              ccGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTc                                                                               <  3:15902/149‑1 (MQ=255)
                                                                gATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTca                                                                              >  4:252998/1‑148 (MQ=255)
                                                                       tAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTc                                                                       >  4:366388/1‑148 (MQ=255)
                                                                        aGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCAGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTTCAGCCATGAAAGATCACACCGCATCGCGGCTGCTCACACCCTc                                                                       >  6:289184/1‑147 (MQ=255)
                                                                         ggCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCa                                                                     <  3:344258/148‑1 (MQ=255)
                                                                                 gaaTTCTAAGCAAGCTTTTTACTGATTGAATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACCGGTTGCAGCCATGAAAGATCACACCCCCTCGCGGCTGCTAACACCCAACACCAAcccgc                                                            >  8:23087/3‑146 (MQ=255)
                                                                                    ttGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCa                                                          <  4:120049/148‑1 (MQ=255)
                                                                                         agcaagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCa                                                                     <  7:355325/132‑1 (MQ=255)
                                                                                         agcaagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCa                                                                     >  8:355325/1‑132 (MQ=255)
                                                                                                  tttACTGATTGTACACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGt                                             >  5:71645/1‑147 (MQ=255)
                                                                                                   ttACTGATTGTTTACAGCTCGATGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCGGCGAAGAGGCCGTCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTa                                           <  7:223942/148‑1 (MQ=255)
                                                                                                   ttACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTa                                           <  5:312889/148‑1 (MQ=255)
                                                                                                            gTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTc                                  >  1:124877/1‑148 (MQ=255)
                                                                                                             tataCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                >  5:132137/1‑149 (MQ=255)
                                                                                                             tataCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                <  5:289184/149‑1 (MQ=255)
                                                                                                              ataCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                <  2:256512/148‑1 (MQ=255)
                                                                                                              ataCAGCTCGAAGTGCCCGTGACGGCCTCTTGGCGGCTAGAGACGCGAAGAGGCCGGCACGGGTTGAAGCCATGAAAGATCAACCCGCCACGCGGCTGCTCACACCCAACAGCAACCGGGCAATCCCCCTCGGGTTAGCGGTTTTCAAc                               >  8:138107/1‑149 (MQ=255)
                                                                                                               ttcAGCTCGAAGTGCCCGTGCCGTCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACTCCTCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAAc                               <  7:23087/146‑1 (MQ=255)
                                                                                                               taCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAAc                               <  3:10224/148‑1 (MQ=255)
                                                                                                               taCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAAc                               <  4:371263/148‑1 (MQ=255)
                                                                                                                  aGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCTGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCAt                           >  3:128993/1‑149 (MQ=255)
                                                                                                                    cTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATcc                         >  7:17142/1‑149 (MQ=255)
                                                                                                                       gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                       <  5:373878/148‑1 (MQ=255)
                                                                                                                             cccGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGt                <  2:364547/149‑1 (MQ=255)
                                                                                                                               cGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGgcag                    <  2:132708/143‑1 (MQ=255)
                                                                                                                               cGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCAGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGgcag                    >  1:132708/1‑143 (MQ=255)
                                                                                                                                 tGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTg               >  3:326469/1‑146 (MQ=255)
                                                                                                                                 tGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTg               <  4:326469/146‑1 (MQ=255)
                                                                                                                                    cGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAg         >  2:264229/1‑149 (MQ=255)
                                                                                                                                    cGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAg         >  1:365471/1‑149 (MQ=255)
                                                                                                                                        ctctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACGGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg       >  1:109411/1‑147 (MQ=39)
                                                                                                                                        ctctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg       >  3:147826/1‑147 (MQ=255)
                                                                                                                                         tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAg    >  6:382320/1‑149 (MQ=255)
                                                                                                                                         tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAg    >  2:201414/1‑149 (MQ=255)
                                                                                                                                           ttGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGtt  >  6:350015/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
AGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTT  >  NC_002947/2951193‑2951480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑