Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,958,807 G→C 16.4% intergenic (+73/+69) PP_2589 → / ← PP_2590 aldehyde dehydrogenase family protein/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,958,8070GC16.4% 114.3 / 14.1 48intergenic (+73/+69)PP_2589/PP_2590aldehyde dehydrogenase family protein/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (24/16);  new base C (5/3);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

CGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACC  >  NC_002947/2958665‑2958950
                                                                                                                                              |                                                                                                                                               
cgATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGc                                                                                                                                           <  5:80053/149‑1 (MQ=37)
       tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCt                                                                                                                                          <  8:209393/143‑1 (MQ=255)
       tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGAc                                                                                                                                      <  3:91940/147‑1 (MQ=255)
         aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                  <  7:309046/149‑1 (MQ=25)
         aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                                  >  1:349769/1‑149 (MQ=255)
         aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTAGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                                  <  2:299185/149‑1 (MQ=255)
            gCAATCCTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTggg                                                                                                                               <  4:73202/149‑1 (MQ=255)
             cATTCGTTCGACAAGTATTCGCAACTCAAGTCCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTAGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTATGAGGGTTGTGCTGTACCTGTGGGa                                                                                                                              <  7:5001/149‑1 (MQ=255)
             cATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                                <  4:217605/147‑1 (MQ=255)
                  gttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGGTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCgg                                                                                                                          <  5:3548/148‑1 (MQ=255)
                       aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGgcgcgc                                                                                                                    >  6:89830/1‑149 (MQ=255)
                        cAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCCTGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGGTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                                   >  8:334242/1‑149 (MQ=255)
                           gTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCcgc                                                                                                                <  2:65980/149‑1 (MQ=255)
                                      tCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCCGCACAACTCTGAGGGTTGTGCTGGCCCGGGGGGAGCGGGCGCGCCCGCGCAGAGGCCg                                                                                                      >  4:962/1‑148 (MQ=39)
                                          gACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                  >  6:250876/1‑116 (MQ=18)
                                          gACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                  <  5:250876/116‑1 (MQ=18)
                                                        aGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGcggcgg                                                                                    >  4:77699/1‑148 (MQ=255)
                                                         gTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGa                                                                                  >  5:221338/1‑149 (MQ=255)
                                                                     aTGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACCGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGaa                                                                       <  2:285083/148‑1 (MQ=255)
                                                                     aTGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACACGGCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGaa                                                                       <  5:348247/148‑1 (MQ=255)
                                                                          ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGaa                                                                       >  1:301175/1‑143 (MQ=255)
                                                                          ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCCGCGGGACCCGGGTAGAAGTCCAc                                                                 >  7:134319/1‑149 (MQ=255)
                                                                                aTTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGc                                                           >  8:200064/1‑149 (MQ=255)
                                                                                    aCCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGa                                                        >  7:218760/1‑148 (MQ=255)
                                                                                       gcgTCGTCTGCTGCGCGGGCATGCCCGCTCCCACCGGTTCCGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGc                                                    <  2:266618/149‑1 (MQ=255)
                                                                                                 cTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGGCCCGGTTAGAAGTCCACGCTAGGCGAAAGCTGATAGGTcc                                          >  7:181557/1‑149 (MQ=255)
                                                                                                  ttCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGaaa                                                      >  8:177600/1‑136 (MQ=255)
                                                                                                  ttCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGaaa                                                      <  7:177600/136‑1 (MQ=255)
                                                                                                    cgcgGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCcgcg                                       >  5:110281/1‑149 (MQ=255)
                                                                                                       gggCATGCCCGCTCCCACTGGTTCCTCACTAGTCTTCGGGTTGTGCTGTACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCgcggcg                                    <  8:25843/149‑1 (MQ=38)
                                                                                                                     ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                      >  5:48093/1‑149 (MQ=255)
                                                                                                                       acaGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGt                    >  3:304224/1‑149 (MQ=255)
                                                                                                                       acaGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCTAAGAGGCCGTATCTGGCGCCGCGGCGGGAACCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGt                    >  4:345899/1‑149 (MQ=37)
                                                                                                                         aGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCt                  >  7:175949/1‑149 (MQ=37)
                                                                                                                         aGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTATCCGAAAGCTGATAGGTCAGCGGCGCCCCCAACCCCAGGGTCt                  >  1:36261/1‑149 (MQ=25)
                                                                                                                           gtccAGCACAACCCTCAGACTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCCGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTgg                >  6:271026/4‑149 (MQ=38)
                                                                                                                              cAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGt              >  8:260501/1‑148 (MQ=255)
                                                                                                                               aGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCa            <  7:260501/149‑1 (MQ=255)
                                                                                                                                gCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAg           >  5:121322/1‑149 (MQ=255)
                                                                                                                                 cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAGACCCTGGGGTTgggggcgc          <  5:89830/149‑6 (MQ=39)
                                                                                                                                 cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGt          <  7:200064/149‑1 (MQ=255)
                                                                                                                                 cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGACGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGt          >  5:170869/1‑149 (MQ=255)
                                                                                                                                    aaGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTgg        >  6:251181/1‑148 (MQ=255)
                                                                                                                                    aaGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGc       >  8:128593/1‑149 (MQ=255)
                                                                                                                                    aaGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCACGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCAGGTTAGAAGACCACGCTAGCACAAAGCTGATAGGTACGCAGCGCCCCCAACCCCATGGTATTGGTCAGTgg        >  8:69230/1‑148 (MQ=255)
                                                                                                                                      gTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGca      >  8:42121/1‑148 (MQ=255)
                                                                                                                                       tCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGcaca    >  5:114784/1‑149 (MQ=255)
                                                                                                                                         tGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCGGGGTCTGGGTCAGTGGCACAcc  >  6:171486/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACC  >  NC_002947/2958665‑2958950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑