Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,958,816 G→A 13.3% intergenic (+82/+60) PP_2589 → / ← PP_2590 aldehyde dehydrogenase family protein/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,958,8160GA13.3% 110.0 / 7.8 47intergenic (+82/+60)PP_2589/PP_2590aldehyde dehydrogenase family protein/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (26/13);  new base A (4/2);  total (30/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01

TCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCAGTAGTCCTTGT  >  NC_002947/2958672‑2958964
                                                                                                                                                |                                                                                                                                                    
tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGAc                                                                                                                                                    <  3:91940/147‑1 (MQ=255)
  aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                                <  7:309046/149‑1 (MQ=25)
  aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                                                >  1:349769/1‑149 (MQ=255)
  aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTAGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                                                <  2:299185/149‑1 (MQ=255)
     gCAATCCTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTggg                                                                                                                                             <  4:73202/149‑1 (MQ=255)
      cATTCGTTCGACAAGTATTCGCAACTCAAGTCCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTAGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTATGAGGGTTGTGCTGTACCTGTGGGa                                                                                                                                            <  7:5001/149‑1 (MQ=255)
      cATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                                              <  4:217605/147‑1 (MQ=255)
           gttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGGTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCgg                                                                                                                                        <  5:3548/148‑1 (MQ=255)
                aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGgcgcgc                                                                                                                                  >  6:89830/1‑149 (MQ=255)
                 cAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCCTGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGGTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                                                 >  8:334242/1‑149 (MQ=255)
                    gTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCcgc                                                                                                                              <  2:65980/149‑1 (MQ=255)
                               tCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCCGCACAACTCTGAGGGTTGTGCTGGCCCGGGGGGAGCGGGCGCGCCCGCGCAGAGGCCg                                                                                                                    >  4:962/1‑148 (MQ=39)
                                   gACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                                >  6:250876/1‑116 (MQ=18)
                                   gACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                                <  5:250876/116‑1 (MQ=18)
                                                 aGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGcggcgg                                                                                                  >  4:77699/1‑148 (MQ=255)
                                                  gTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGa                                                                                                >  5:221338/1‑149 (MQ=255)
                                                              aTGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACCGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGaa                                                                                     <  2:285083/148‑1 (MQ=255)
                                                              aTGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACACGGCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGaa                                                                                     <  5:348247/148‑1 (MQ=255)
                                                                   ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGaa                                                                                     >  1:301175/1‑143 (MQ=255)
                                                                   ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCCGCGGGACCCGGGTAGAAGTCCAc                                                                               >  7:134319/1‑149 (MQ=255)
                                                                         aTTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGc                                                                         >  8:200064/1‑149 (MQ=255)
                                                                             aCCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGa                                                                      >  7:218760/1‑148 (MQ=255)
                                                                                gcgTCGTCTGCTGCGCGGGCATGCCCGCTCCCACCGGTTCCGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGc                                                                  <  2:266618/149‑1 (MQ=255)
                                                                                          cTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGGCCCGGTTAGAAGTCCACGCTAGGCGAAAGCTGATAGGTcc                                                        >  7:181557/1‑149 (MQ=255)
                                                                                           ttCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGaaa                                                                    >  8:177600/1‑136 (MQ=255)
                                                                                           ttCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGaaa                                                                    <  7:177600/136‑1 (MQ=255)
                                                                                             cgcgGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCcgcg                                                     >  5:110281/1‑149 (MQ=255)
                                                                                                gggCATGCCCGCTCCCACTGGTTCCTCACTAGTCTTCGGGTTGTGCTGTACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCgcggcg                                                  <  8:25843/149‑1 (MQ=38)
                                                                                                              ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                                    >  5:48093/1‑149 (MQ=255)
                                                                                                                acaGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGt                                  >  3:304224/1‑149 (MQ=255)
                                                                                                                acaGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCTAAGAGGCCGTATCTGGCGCCGCGGCGGGAACCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGt                                  >  4:345899/1‑149 (MQ=37)
                                                                                                                  aGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCt                                >  7:175949/1‑149 (MQ=37)
                                                                                                                  aGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTATCCGAAAGCTGATAGGTCAGCGGCGCCCCCAACCCCAGGGTCt                                >  1:36261/1‑149 (MQ=25)
                                                                                                                    gtccAGCACAACCCTCAGACTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCCGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTgg                              >  6:271026/4‑149 (MQ=38)
                                                                                                                       cAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGt                            >  8:260501/1‑148 (MQ=255)
                                                                                                                        aGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCa                          <  7:260501/149‑1 (MQ=255)
                                                                                                                         gCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAg                         >  5:121322/1‑149 (MQ=255)
                                                                                                                          cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAGACCCTGGGGTTgggggcgc                        <  5:89830/149‑6 (MQ=39)
                                                                                                                          cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGt                        <  7:200064/149‑1 (MQ=255)
                                                                                                                          cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGACGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGt                        >  5:170869/1‑149 (MQ=255)
                                                                                                                             aaGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTgg                      >  6:251181/1‑148 (MQ=255)
                                                                                                                             aaGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGc                     >  8:128593/1‑149 (MQ=255)
                                                                                                                             aaGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCACGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCAGGTTAGAAGACCACGCTAGCACAAAGCTGATAGGTACGCAGCGCCCCCAACCCCATGGTATTGGTCAGTgg                      >  8:69230/1‑148 (MQ=255)
                                                                                                                               gTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGca                    >  8:42121/1‑148 (MQ=255)
                                                                                                                                tCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGcaca                  >  5:114784/1‑149 (MQ=255)
                                                                                                                                  tGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCGGGGTCTGGGTCAGTGGCACAcc                >  6:171486/1‑149 (MQ=255)
                                                                                                                                        ttGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCagt           >  8:330563/1‑148 (MQ=255)
                                                                                                                                                gACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCAGTAGTCCTTGt  >  1:262536/1‑149 (MQ=255)
                                                                                                                                                gACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCAGTAGTCCTTGt  >  8:78695/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCAGTAGTCCTTGT  >  NC_002947/2958672‑2958964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑