Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	304,433	A→G	5.2%	L113L (TTG→CTG)	PP_0249 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	304,433	0	A	G	5.2%	231.6 / 2.2	77	L113L (TTG→CTG)	PP_0249	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (37/36);  new base G (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

GGCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAG  >  NC_002947/304289‑304580                                                                                                                                                 |                                                                                                                                                    ggCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGc                                                                                                                                                 <  4:212763/149‑1 (MQ=255)   cAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGcac                                                                                                                                               <  8:64325/149‑1 (MQ=255)      gCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGcaccac                                                                                                                                            >  2:276610/1‑149 (MQ=255)        cAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGTGCAGTTGCGCCACCg                                                                                                                                          <  3:199022/149‑1 (MQ=255)        cAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTAACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCaccacc                                                                                                                                           >  1:17674/1‑148 (MQ=255)        cAGGGTCAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGTGCAGTTGCGCCACCg                                                                                                                                          <  2:135968/149‑1 (MQ=255)         aGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCACTGACGTGCCGATCACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGa                                                                                                                                         <  1:312512/149‑1 (MQ=255)           ggTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGc                                                                                                                                       >  7:43866/1‑149 (MQ=255)                 cTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGt                                                                                                                                 <  8:191206/149‑1 (MQ=255)                      ccTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGcaca                                                                                                                            <  3:117355/149‑1 (MQ=255)                           cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                         >  6:124822/1‑147 (MQ=255)                           cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                         >  1:209683/1‑147 (MQ=255)                           cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                         >  7:28758/1‑147 (MQ=255)                           cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                       <  6:64205/149‑1 (MQ=255)                           cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                       <  7:228274/149‑1 (MQ=255)                                acCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGaa                                                                                                                  >  3:344674/1‑149 (MQ=255)                                acCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGGCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGaa                                                                                                                  <  6:251787/149‑1 (MQ=255)                                 ccATCCCCCAGCGCCAGTGACGTGCGGAAGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGAGCCTCGCAGCGGCGGTGGCGCAACTGCACCAACGAGCCCAGCTCCGCAGTGAAGCGCAc                                                                                                                 >  7:117752/1‑149 (MQ=255)                                  cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGGTCTAGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                >  8:82115/1‑149 (MQ=255)                                  cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                >  7:103323/1‑149 (MQ=255)                                  cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                >  7:98035/1‑149 (MQ=255)                                  cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATAGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                >  6:378869/1‑149 (MQ=255)                                      aCCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCa                                                                                                              >  2:352680/1‑147 (MQ=255)                                      aCCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCa                                                                                                              <  1:352680/147‑1 (MQ=255)                                        ccAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGc                                                                                                          >  1:36913/1‑149 (MQ=255)                                             gcCAGGGACGTGCTGATGACGTATCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATTGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAtggctggc                                                                                                      <  2:80282/148‑1 (MQ=255)                                                    aCGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCgcgc                                                                                              <  2:36913/149‑1 (MQ=255)                                                         cGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGaa                                                                                         <  6:61359/149‑1 (MQ=255)                                                               aCGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                   <  1:276610/149‑1 (MQ=255)                                                                cGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                   >  3:283130/1‑148 (MQ=255)                                                                     cGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTc                                                                             <  4:283130/149‑1 (MQ=255)                                                                       ggTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCgg                                                                           <  3:358441/149‑1 (MQ=255)                                                                       ggTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCgg                                                                           <  7:345927/149‑1 (MQ=255)                                                                         tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                         >  6:361655/1‑149 (MQ=255)                                                                         tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                         <  6:140111/149‑1 (MQ=255)                                                                           tGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTgg                                                                        >  1:164919/1‑148 (MQ=255)                                                                            tggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                   >  6:290373/2‑136 (MQ=255)                                                                            tggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                   <  5:290373/135‑1 (MQ=255)                                                                             ggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACTGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGc                                                                       <  6:109119/147‑1 (MQ=255)                                                                             ggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCAt                                                                     <  8:43866/149‑1 (MQ=255)                                                                                gTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCa                                                                  <  5:124822/149‑1 (MQ=255)                                                                                gTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGGTCCCCTTCGGTGGCATGcg                                                                  >  8:156063/1‑148 (MQ=255)                                                                                   gCCGTGGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAgg                                                                <  7:110573/148‑1 (MQ=255)                                                                                      gTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTc                                                             >  2:199520/1‑148 (MQ=255)                                                                                       ttGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGa                                                           <  4:344674/149‑1 (MQ=255)                                                                                         ggAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGAc                                                          <  4:302779/148‑1 (MQ=255)                                                                                                  gATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAc                                                >  1:278337/1‑149 (MQ=255)                                                                                                   aTGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAcc                                               >  8:60079/1‑149 (MQ=255)                                                                                                    tGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGc                                                      <  8:339132/141‑1 (MQ=255)                                                                                                    tGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGc                                                      >  7:339132/1‑141 (MQ=255)                                                                                                        tttCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGgtgt                                          >  5:145305/1‑149 (MQ=255)                                                                                                          tCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTc                                         <  8:28758/148‑1 (MQ=255)                                                                                                               gTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCt                                    <  1:199520/148‑1 (MQ=255)                                                                                                               gTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCt                                    <  2:164919/148‑1 (MQ=255)                                                                                                                 cACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTgg                                 >  3:295354/1‑149 (MQ=255)                                                                                                                  aCCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGAc                                                          <  2:196814/123‑1 (MQ=255)                                                                                                                  aCCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGAc                                                          >  1:196814/1‑123 (MQ=255)                                                                                                                  aCCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgc                                >  5:179187/1‑149 (MQ=255)                                                                                                                    cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGtt                                                                                    >  2:135263/1‑95 (MQ=255)                                                                                                                    cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGtt                                                                                    <  1:135263/95‑1 (MQ=255)                                                                                                                     aaCGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgcg                             >  3:193927/1‑149 (MQ=255)                                                                                                                              tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc                    >  4:314441/1‑149 (MQ=255)                                                                                                                              tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc                    >  2:345454/1‑149 (MQ=255)                                                                                                                              tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc                    >  6:205838/1‑149 (MQ=255)                                                                                                                                  aGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTg                                  <  7:147775/131‑1 (MQ=255)                                                                                                                                  aGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTg                                  >  8:147775/1‑131 (MQ=255)                                                                                                                                    cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCGGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGcc              >  3:140214/1‑149 (MQ=255)                                                                                                                                    cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                              <  5:198416/133‑1 (MQ=255)                                                                                                                                    cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                              >  6:198416/1‑133 (MQ=255)                                                                                                                                    cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGcc              <  5:378869/149‑1 (MQ=255)                                                                                                                                     ggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTg                                                    >  4:202181/1‑110 (MQ=255)                                                                                                                                     ggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTg                                                    <  3:202181/110‑1 (MQ=255)                                                                                                                                      tcggTGGTGCAGTTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGcc              >  3:304281/2‑147 (MQ=255)                                                                                                                                        ggtggtGCAGTTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAgg          >  5:360859/1‑149 (MQ=255)                                                                                                                                            gcgcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcag      <  6:145305/149‑1 (MQ=255)                                                                                                                                            gcgcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTca       <  2:209683/148‑1 (MQ=255)                                                                                                                                              gcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcagg     >  2:300717/1‑148 (MQ=255)                                                                                                                                                aaCTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcaggcag  >  1:199086/1‑149 (MQ=255)                                                                                                                                                aaCTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcaggcag  <  7:60079/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    GGCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAG  >  NC_002947/304289‑304580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑