Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,048,590 A→G 7.7% intergenic (+45/‑251) PP_2661 → / → PP_2662 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,048,5900AG 7.7% 146.9 / 2.6 52intergenic (+45/‑251)PP_2661/PP_2662hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base G (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

AGGTGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGG  >  NC_002947/3048442‑3048727
                                                                                                                                                    |                                                                                                                                         
aGGTGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCa                                                                                                                                           <  2:255306/149‑1 (MQ=255)
 ggTGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAg                                                                                                                                          >  6:359716/1‑149 (MQ=255)
 ggTGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAg                                                                                                                                          <  8:74796/149‑1 (MQ=255)
 ggTGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAg                                                                                                                                          >  2:120378/1‑149 (MQ=255)
   tGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGcc                                                                                                                                        >  8:203823/1‑149 (MQ=255)
      aGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCtt                                                                                                                                     >  3:335683/1‑149 (MQ=255)
       ggACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTc                                                                                                                                    >  4:118072/1‑149 (MQ=255)
       ggACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTc                                                                                                                                    >  5:104640/1‑149 (MQ=255)
               tggtggATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGa                                                                                                                            <  1:148927/149‑1 (MQ=255)
                 gtggATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGc                                                                                                                          <  6:104640/149‑1 (MQ=255)
                 gtggATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGc                                                                                                                          >  7:195253/1‑149 (MQ=255)
                       gtgCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGa                                                                                                                    <  1:136128/149‑1 (MQ=255)
                        tgCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCcgcg                                                                                                                     >  6:304206/1‑147 (MQ=255)
                        tgCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTGTGCAGCCCTTCGCGGGCGAGCCCGCGaa                                                                                                                   <  7:86795/149‑1 (MQ=255)
                         gCAGGCACGGCTGGCCTTGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCTGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTAAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGaa                                                                                                                   <  6:74574/148‑1 (MQ=255)
                          cAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAgg                                                                                                                 <  8:195253/149‑1 (MQ=255)
                          cAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAgg                                                                                                                 <  8:2100/149‑1 (MQ=255)
                          cAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAgg                                                                                                                 >  5:367343/1‑149 (MQ=39)
                             gCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGcc                                                                                                              >  1:112665/1‑149 (MQ=255)
                             gCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGcc                                                                                                              >  3:119680/1‑149 (MQ=255)
                               aCGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAGGGctg                                                                                                             >  4:63618/1‑146 (MQ=38)
                                  gCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCGTCGCGGGCGAGCCCGCGAAGGGCCGCAAg                                                                                                         <  7:41009/149‑1 (MQ=255)
                                      gcctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAggc                                                                                                       <  6:88091/147‑1 (MQ=255)
                                       cctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAggcggc                                                                                                    <  4:119680/149‑1 (MQ=255)
                                        ctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGcc                                                                                                   >  3:36273/1‑149 (MQ=255)
                                        ctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAGGGCTGCAAAGCAGcc                                                                                                   <  3:63618/149‑1 (MQ=37)
                                           gcagcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCg                                                                                                >  6:64805/1‑149 (MQ=255)
                                              gcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAGGGCTGCAAAGCAGccccaatt                                                                                             <  6:367343/149‑5 (MQ=25)
                                                  tcgcaatgTTATGAAATGCACGCTTGCCAGCGTTCGCAGGCATTTGCTTCCTGAAGCCAGGAGATTCCGCTCATATAATGTAATTGGGGCTGGTTTGCAGCCCTTCGCGGGCGAGCCGGCGAAGGGCCGCAAGGCGTCCCCGGttcatt                                                                                         <  8:154304/142‑1 (MQ=255)
                                                     cACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCATTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCCGCCCCGGttcattca                                                                                       >  8:77168/1‑148 (MQ=255)
                                                       ccGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATc                                                                                    <  8:275695/149‑1 (MQ=255)
                                                          ttATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTa                                                                                 <  7:203823/149‑1 (MQ=255)
                                                          ttATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTa                                                                                 <  3:118072/149‑1 (MQ=255)
                                                             tGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTAtt                                                                               <  2:112665/148‑1 (MQ=255)
                                                             tGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTAtt                                                                               >  3:48618/1‑148 (MQ=255)
                                                             tGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTAtt                                                                               >  7:29107/1‑148 (MQ=255)
                                                                             cAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCGCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACt                                                              >  8:223171/1‑149 (MQ=255)
                                                                             cAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACt                                                              >  6:136717/1‑149 (MQ=255)
                                                                                  ttCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctgc                                                         <  5:304206/149‑1 (MQ=255)
                                                                                     gatgggaCTTGCTTCCTGATGCCATGAGATTCCGCTCAGATAATGTAATTGTGTCTGCTTTGCAGCCCTTAGCTGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctgcct                                                       <  5:359716/142‑1 (MQ=255)
                                                                                        ggCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGCGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGc                                                   <  1:120378/149‑1 (MQ=255)
                                                                                            cTGGCTTCAGGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGTGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTAATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCAt                                                >  7:234151/1‑148 (MQ=255)
                                                                                               gCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGt                                            <  5:136717/149‑1 (MQ=255)
                                                                                                cTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGtc                                           <  1:21871/149‑1 (MQ=255)
                                                                                                      gAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCAGCGAAGGGCCGCAAGGCGGCACCGGTTCATTCAATCGTATTGATACCCGACTGCCACTCCTGACTGAGACATAAGTCTAGAAc                                     >  5:358204/1‑149 (MQ=255)
                                                                                                       aaGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTACTGCCTGAGCCATAAGTCTCGAAc                                     >  1:360750/1‑148 (MQ=255)
                                                                                                                gaTTCCGCTCACATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCaa                           <  8:286669/149‑1 (MQ=255)
                                                                                                                          aGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCg                 <  4:335683/149‑1 (MQ=255)
                                                                                                                                tGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCa                                   >  8:105394/1‑125 (MQ=255)
                                                                                                                                tGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCa                                   <  7:105394/125‑1 (MQ=255)
                                                                                                                                  tAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCaaa         >  7:286768/1‑149 (MQ=255)
                                                                                                                                        gggCTGCTTTGCAGCGCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCAcc    <  7:223171/148‑1 (MQ=255)
                                                                                                                                         ggCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCgg  <  4:36273/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
AGGTGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGG  >  NC_002947/3048442‑3048727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑