Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 304,438 A→G 6.4% V111A (GTG→GCG)  PP_0249 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947304,4380AG 6.4% 227.3 / 4.3 78V111A (GTG→GCG) PP_0249hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/37);  new base G (3/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

CAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAGGGCGA  >  NC_002947/304291‑304585
                                                                                                                                                   |                                                                                                                                                   
cAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGcac                                                                                                                                                    <  8:64325/149‑1 (MQ=255)
   gCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGcaccac                                                                                                                                                 >  2:276610/1‑149 (MQ=255)
     cAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGTGCAGTTGCGCCACCg                                                                                                                                               <  3:199022/149‑1 (MQ=255)
     cAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTAACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCaccacc                                                                                                                                                >  1:17674/1‑148 (MQ=255)
     cAGGGTCAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGTGCAGTTGCGCCACCg                                                                                                                                               <  2:135968/149‑1 (MQ=255)
      aGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCACTGACGTGCCGATCACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGa                                                                                                                                              <  1:312512/149‑1 (MQ=255)
        ggTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGc                                                                                                                                            >  7:43866/1‑149 (MQ=255)
              cTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGt                                                                                                                                      <  8:191206/149‑1 (MQ=255)
                   ccTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGcaca                                                                                                                                 <  3:117355/149‑1 (MQ=255)
                        cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                              >  1:209683/1‑147 (MQ=255)
                        cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                              >  6:124822/1‑147 (MQ=255)
                        cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                              >  7:28758/1‑147 (MQ=255)
                        cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                            <  6:64205/149‑1 (MQ=255)
                        cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                            <  7:228274/149‑1 (MQ=255)
                             acCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGaa                                                                                                                       >  3:344674/1‑149 (MQ=255)
                             acCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGGCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGaa                                                                                                                       <  6:251787/149‑1 (MQ=255)
                              ccATCCCCCAGCGCCAGTGACGTGCGGAAGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGAGCCTCGCAGCGGCGGTGGCGCAACTGCACCAACGAGCCCAGCTCCGCAGTGAAGCGCAc                                                                                                                      >  7:117752/1‑149 (MQ=255)
                               cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGGTCTAGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                     >  8:82115/1‑149 (MQ=255)
                               cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                     >  7:103323/1‑149 (MQ=255)
                               cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                     >  7:98035/1‑149 (MQ=255)
                               cATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATAGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAAcc                                                                                                                     >  6:378869/1‑149 (MQ=255)
                                   aCCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCa                                                                                                                   >  2:352680/1‑147 (MQ=255)
                                   aCCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCa                                                                                                                   <  1:352680/147‑1 (MQ=255)
                                     ccAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGc                                                                                                               >  1:36913/1‑149 (MQ=255)
                                          gcCAGGGACGTGCTGATGACGTATCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATTGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAtggctggc                                                                                                           <  2:80282/148‑1 (MQ=255)
                                                 aCGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCgcgc                                                                                                   <  2:36913/149‑1 (MQ=255)
                                                      cGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGaa                                                                                              <  6:61359/149‑1 (MQ=255)
                                                            aCGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                        <  1:276610/149‑1 (MQ=255)
                                                             cGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                        >  3:283130/1‑148 (MQ=255)
                                                                  cGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTc                                                                                  <  4:283130/149‑1 (MQ=255)
                                                                    ggTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCgg                                                                                <  7:345927/149‑1 (MQ=255)
                                                                    ggTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCgg                                                                                <  3:358441/149‑1 (MQ=255)
                                                                      tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                              >  6:361655/1‑149 (MQ=255)
                                                                      tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                              <  6:140111/149‑1 (MQ=255)
                                                                        tGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTgg                                                                             >  1:164919/1‑148 (MQ=255)
                                                                         tggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                        >  6:290373/2‑136 (MQ=255)
                                                                         tggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTc                                                                                        <  5:290373/135‑1 (MQ=255)
                                                                          ggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACTGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGc                                                                            <  6:109119/147‑1 (MQ=255)
                                                                          ggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCAt                                                                          <  8:43866/149‑1 (MQ=255)
                                                                             gTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCa                                                                       <  5:124822/149‑1 (MQ=255)
                                                                             gTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGGTCCCCTTCGGTGGCATGcg                                                                       >  8:156063/1‑148 (MQ=255)
                                                                                gCCGTGGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAgg                                                                     <  7:110573/148‑1 (MQ=255)
                                                                                   gTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTc                                                                  >  2:199520/1‑148 (MQ=255)
                                                                                    ttGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGa                                                                <  4:344674/149‑1 (MQ=255)
                                                                                      ggAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGAc                                                               <  4:302779/148‑1 (MQ=255)
                                                                                               gATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAc                                                     >  1:278337/1‑149 (MQ=255)
                                                                                                aTGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAcc                                                    >  8:60079/1‑149 (MQ=255)
                                                                                                 tGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGc                                                           <  8:339132/141‑1 (MQ=255)
                                                                                                 tGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGc                                                           >  7:339132/1‑141 (MQ=255)
                                                                                                     tttCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGgtgt                                               >  5:145305/1‑149 (MQ=255)
                                                                                                       tCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTc                                              <  8:28758/148‑1 (MQ=255)
                                                                                                            gTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCt                                         <  1:199520/148‑1 (MQ=255)
                                                                                                            gTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCt                                         <  2:164919/148‑1 (MQ=255)
                                                                                                              cACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTgg                                      >  3:295354/1‑149 (MQ=255)
                                                                                                               aCCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGAc                                                               >  1:196814/1‑123 (MQ=255)
                                                                                                               aCCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGAc                                                               <  2:196814/123‑1 (MQ=255)
                                                                                                               aCCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgc                                     >  5:179187/1‑149 (MQ=255)
                                                                                                                 cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGtt                                                                                         >  2:135263/1‑95 (MQ=255)
                                                                                                                 cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGtt                                                                                         <  1:135263/95‑1 (MQ=255)
                                                                                                                  aaCGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgcg                                  >  3:193927/1‑149 (MQ=255)
                                                                                                                           tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc                         >  2:345454/1‑149 (MQ=255)
                                                                                                                           tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc                         >  4:314441/1‑149 (MQ=255)
                                                                                                                           tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc                         >  6:205838/1‑149 (MQ=255)
                                                                                                                               aGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTg                                       <  7:147775/131‑1 (MQ=255)
                                                                                                                               aGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTg                                       >  8:147775/1‑131 (MQ=255)
                                                                                                                                 cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCGGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGcc                   >  3:140214/1‑149 (MQ=255)
                                                                                                                                 cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                                   <  5:198416/133‑1 (MQ=255)
                                                                                                                                 cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                                   >  6:198416/1‑133 (MQ=255)
                                                                                                                                 cggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGcc                   <  5:378869/149‑1 (MQ=255)
                                                                                                                                  ggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTg                                                         >  4:202181/1‑110 (MQ=255)
                                                                                                                                  ggcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTg                                                         <  3:202181/110‑1 (MQ=255)
                                                                                                                                   tcggTGGTGCAGTTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGcc                   >  3:304281/2‑147 (MQ=255)
                                                                                                                                     ggtggtGCAGTTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAgg               >  5:360859/1‑149 (MQ=255)
                                                                                                                                         gcgcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcag           <  6:145305/149‑1 (MQ=255)
                                                                                                                                         gcgcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTca            <  2:209683/148‑1 (MQ=255)
                                                                                                                                           gcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcagg          >  2:300717/1‑148 (MQ=255)
                                                                                                                                             aaCTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcaggcag       >  1:199086/1‑149 (MQ=255)
                                                                                                                                             aaCTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTcaggcag       <  7:60079/149‑1 (MQ=255)
                                                                                                                                               cTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAggg     <  1:345454/149‑1 (MQ=255)
                                                                                                                                               cTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAggg     <  6:5104/149‑1 (MQ=255)
                                                                                                                                                 gCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAGGGCg   >  5:12537/1‑149 (MQ=255)
                                                                                                                                                  caccacCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAGGGCGa  >  6:273675/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCAGGCAGGGCGA  >  NC_002947/304291‑304585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑