Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,088,966 T→C 7.2% S63G (AGC→GGC)  PP_2697 ← flavin reductase domain‑containing oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,088,9660TC 7.2% 151.4 / 2.5 56S63G (AGC→GGC) PP_2697flavin reductase domain‑containing oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (22/29);  new base C (2/2);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

GATCGGGTTGCCCAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCACCGTGATGCCGGATGCGTAGTGCCCGAGTGCCTGG  >  NC_002947/3088820‑3089111
                                                                                                                                                   |                                                                                                                                                  
gATCGGGTTGCCCAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTgg                                                                                                                                                  >  1:6327/1‑149 (MQ=255)
gATCGGGTTGCCCAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTgg                                                                                                                                                  >  3:53605/1‑149 (MQ=255)
         gCCCAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTg                                                                                                                                          <  8:192267/148‑1 (MQ=255)
          cccAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGa                                                                                                                                        <  5:229624/149‑1 (MQ=255)
          cccAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGa                                                                                                                                        <  1:326761/149‑1 (MQ=255)
          cccAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGa                                                                                                                                        <  2:245661/149‑1 (MQ=255)
            cAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACa                                                                                                                                      >  1:130631/1‑149 (MQ=255)
            cAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCCGGCCGAACTGGACa                                                                                                                                      >  8:328962/1‑149 (MQ=255)
            cAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCCGGCCGAACTGGACa                                                                                                                                      >  2:263234/1‑149 (MQ=255)
              aCGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATc                                                                                                                                    <  2:130631/149‑1 (MQ=255)
              aCGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCCGGCCGAACTGGACATc                                                                                                                                    <  7:328962/149‑1 (MQ=255)
              aCGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCCGGCCGAACTGGACATc                                                                                                                                    <  1:263234/149‑1 (MQ=255)
               cGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATca                                                                                                                                   <  2:120574/149‑1 (MQ=255)
                     ctcTTTCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGGGCAAACTGGTTGGAAATGCCGACCTGC‑TCCCCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTTGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGa                                                                                                                             <  6:277183/147‑1 (MQ=255)
                          gCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGa                                                                                                                         >  4:343522/1‑148 (MQ=255)
                           ccATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TAACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTTGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGac                                                                                                                        <  7:163101/148‑1 (MQ=255)
                            cATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGacac                                                                                                                      <  8:5772/149‑1 (MQ=255)
                                 gACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAgcg                                                                                                                 >  3:175584/1‑149 (MQ=255)
                                      ccTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCggg                                                                                                            <  2:6327/149‑1 (MQ=255)
                                      ccTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCggg                                                                                                            <  7:104785/149‑1 (MQ=255)
                                      ccTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACACCACACTGAATGACACCAGCGGCggg                                                                                                            <  6:150893/149‑1 (MQ=255)
                                          ccACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTc                                                                                                         <  4:175584/148‑1 (MQ=255)
                                           cACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCAt                                                                                                       >  2:104691/1‑149 (MQ=255)
                                           cACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCAt                                                                                                       >  7:52229/1‑149 (MQ=255)
                                                    gcgccgcgccGTGCAAACTGGTTGGAAATGCCGACCTGCATATCCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACg                                                                                                <  1:316276/148‑1 (MQ=255)
                                                    gcgccgcgccGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGc                                                                                               <  3:356216/148‑1 (MQ=255)
                                                      gccgcgccgTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGt                                                                                            <  6:22510/149‑1 (MQ=255)
                                                      gccgcgccgTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCGGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGt                                                                                            <  5:330652/149‑1 (MQ=255)
                                                        cgcgccgTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAg                                                                                          <  5:90010/149‑1 (MQ=255)
                                                           gccgTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAAc                                                                                       >  2:112754/1‑149 (MQ=255)
                                                                    aCTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAg                                                                              >  6:221055/1‑149 (MQ=255)
                                                                    aCTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAg                                                                              <  1:104691/149‑1 (MQ=255)
                                                                      tGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAAc                                                                                       >  1:68080/1‑138 (MQ=255)
                                                                      tGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAAc                                                                                       <  2:68080/138‑1 (MQ=255)
                                                                        gTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTg                                                                           >  7:76668/1‑148 (MQ=255)
                                                                           ggAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGc                                                                       >  1:377907/1‑149 (MQ=255)
                                                                            gAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGcc                                                                      >  6:123526/1‑149 (MQ=255)
                                                                                  ccGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCgg                                                                <  5:129574/149‑1 (MQ=255)
                                                                                         gC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TAACCt                                                        >  1:24783/1‑149 (MQ=255)
                                                                                          c‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTG‑AGCCGATCGGCTTCACCt                                                        <  8:52229/148‑1 (MQ=255)
                                                                                          c‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGA‑TTTCGGGTAGCTGGCCGAACTGGACATGACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTG‑AGCCGATCGGCTTCACCt                                                        <  7:134354/147‑1 (MQ=255)
                                                                                              aCCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTc                                                      <  5:221055/147‑1 (MQ=255)
                                                                                              aCCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCgt                                                    >  6:370692/1‑149 (MQ=255)
                                                                                                cTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCgtgg                                                  >  7:306950/1‑149 (MQ=255)
                                                                                                      ggATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGAt                                            <  1:112754/149‑1 (MQ=255)
                                                                                                       gATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATg                                           >  8:348831/1‑149 (MQ=255)
                                                                                                           ttGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTaa                                        <  4:53605/148‑1 (MQ=255)
                                                                                                              aCCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCAc                                    >  6:126496/1‑149 (MQ=255)
                                                                                                                cGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCACCg                                  >  4:308783/1‑149 (MQ=255)
                                                                                                                           aaGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCACCGTGATGCCGGAt                       <  8:306950/148‑1 (MQ=255)
                                                                                                                                  gCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCACCGTGATGCCGGATGCGTAGt                >  7:85691/1‑149 (MQ=255)
                                                                                                                                      aTTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCACCGTGATGCCGGATGCGTAGTGccc            <  7:157394/149‑1 (MQ=255)
                                                                                                                                         ttCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCACCGTGATGCCGGATGCGTGGTGCCCGa          <  2:377907/148‑1 (MQ=255)
                                                                                                                                              gTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAg                                                                              <  4:177068/76‑1 (MQ=255)
                                                                                                                                              gTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAg                                                                              >  3:177068/1‑76 (MQ=255)
                                                                                                                                                 gCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTTGTGCGATGTAATCACCGTGATGCCGGATGCGTAGTGCCGGAGTGCCTgg  >  3:45775/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATCGGGTTGCCCAACGGCGACGCTTGCCATTCGACGCCCTGCCACTTGTTGGCGCCGCGCCGTGCAAACTGGTTGGAAATGCCGACCTGC‑TCACCTGACAGGATGTTGACCGTGAAGCGGCCAGCCTGGCGGATTTTCGGGTAGCTGGCCGAACTGGACATCACACTGAATGACACCAGCGGCGGGCTCATCGATACGCTGTAGAACGACTGGCAGGTGAAGCCGATCGGC‑TCACCTTCGTGGTGCGATGTAATCACCGTGATGCCGGATGCGTAGTGCCCGAGTGCCTGG  >  NC_002947/3088820‑3089111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑