Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,148,641 C→G 5.2% G193G (GGC→GGG)  msuE → FMN reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,148,6410CG 5.2% 254.2 / 2.9 77G193G (GGC→GGG) msuEFMN reductase
Reads supporting (aligned to +/- strand):  ref base C (36/37);  new base G (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

CTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACAT  >  NC_002947/3148495‑3148789
                                                                                                                                                  |                                                                                                                                                    
cTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCt                                                                                                                                                    <  5:290976/149‑1 (MQ=255)
cTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCt                                                                                                                                                    >  3:164255/1‑149 (MQ=255)
        ccTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCa                                                                                                                                            <  5:247312/149‑1 (MQ=255)
         cTGGTCGATCACCAGTCGCTCAAGGGCGTGCCAGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGATTAGGCAACTATTAGCATTCTTCCAAGCCCACACCCAACCGAACGCCCGGTACGACAg                                                                                                                                           >  1:311202/1‑149 (MQ=255)
               gATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTc                                                                                                                                       >  4:223380/1‑147 (MQ=255)
                 tCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGt                                                                                                                                   <  7:6133/149‑1 (MQ=255)
                  cACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCCCCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGACGAGTc                                                                                                                                  >  7:270907/1‑149 (MQ=255)
                   aCCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCt                                                                                                                                 <  4:75719/149‑1 (MQ=255)
                       gTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTc                                                                                                                              >  5:66929/1‑148 (MQ=255)
                           cTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTcgacga                                                                                                                         >  5:82065/1‑149 (MQ=255)
                            tCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGAt                                                                                                                        >  3:80113/1‑149 (MQ=255)
                              aaGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGAt                                                                                                                        >  3:302166/1‑147 (MQ=255)
                                gggCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAgc                                                                                                                    >  3:284278/1‑149 (MQ=255)
                                  gCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAgcgc                                                                                                                  >  3:3834/1‑149 (MQ=255)
                                    gTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCt                                                                                                                <  4:3834/149‑1 (MQ=255)
                                       cccGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTgg                                                                                                              <  4:226620/148‑1 (MQ=255)
                                        ccGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCAACGATCAGCGCCTGGCt                                                                                                            >  2:9195/1‑149 (MQ=255)
                                                cTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTgc                                                                                                    >  7:91346/1‑149 (MQ=255)
                                                    attctaCCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCa                                                                                                 <  3:195377/143‑1 (MQ=255)
                                                     cgccACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTc                                                                                                                              >  6:302965/1‑118 (MQ=255)
                                                     cgccACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTc                                                                                                                              <  5:302965/118‑1 (MQ=255)
                                                           cGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCGGTTCGAAc                                                                                         <  4:164255/149‑1 (MQ=255)
                                                             gtggtAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACgg                                                                                       <  3:223380/149‑1 (MQ=255)
                                                                gtAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATc                                                                                    <  4:302166/149‑1 (MQ=255)
                                                                      agcgaCATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGGTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCgg                                                                              <  5:85694/149‑1 (MQ=255)
                                                                      agcgaCATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCgg                                                                              <  6:375318/149‑1 (MQ=255)
                                                                         gaCATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTa                                                                           >  4:361744/1‑149 (MQ=255)
                                                                            aTGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTc                                                                        <  4:102271/149‑1 (MQ=255)
                                                                               ccTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGaa                                                                                          <  7:288247/128‑1 (MQ=255)
                                                                               ccTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGaa                                                                                          >  8:288247/1‑128 (MQ=255)
                                                                                   gatgatCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGa                                                                                      <  6:38881/128‑1 (MQ=255)
                                                                                   gatgatCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGa                                                                                      >  5:38881/1‑128 (MQ=255)
                                                                                     tgatCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTc                                                               >  5:206742/1‑149 (MQ=255)
                                                                                      gatcgatcACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCg                                                              >  5:94918/1‑149 (MQ=255)
                                                                                       atcgatcACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCgg                                                             >  7:124169/1‑149 (MQ=255)
                                                                                        tcgatcACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCgg                                                                              <  7:181927/131‑1 (MQ=255)
                                                                                        tcgatcACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCgg                                                                              >  8:181927/1‑131 (MQ=255)
                                                                                         cgatcACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATc                                                                                    >  5:213603/1‑124 (MQ=255)
                                                                                         cgatcACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATc                                                                                    <  6:213603/124‑1 (MQ=255)
                                                                                          gatcACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGgcgc                                                          >  7:346666/1‑149 (MQ=255)
                                                                                                 aGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCAAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCttt                                                   <  8:340224/149‑1 (MQ=255)
                                                                                                        cccGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGcc                                                         >  7:295949/1‑136 (MQ=255)
                                                                                                        cccGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGcc                                                         <  8:295949/136‑1 (MQ=255)
                                                                                                          cGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCttt                                                   <  8:321786/140‑1 (MQ=255)
                                                                                                          cGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCttt                                                   >  7:321786/1‑140 (MQ=255)
                                                                                                             tATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTc                                       >  3:354871/1‑149 (MQ=255)
                                                                                                               ttCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTc                                       <  6:206742/147‑1 (MQ=255)
                                                                                                               ttCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGc                                     <  7:41690/149‑1 (MQ=255)
                                                                                                                   cTTTCTTCCAGGCCCACACGCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGCTCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCcgc                                 <  4:284278/149‑1 (MQ=255)
                                                                                                                   cTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCcgc                                 >  8:183976/1‑149 (MQ=255)
                                                                                                                   cTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCAACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCcgc                                 <  1:9195/149‑1 (MQ=255)
                                                                                                                    tttCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGa                                                                                                          <  6:260771/75‑1 (MQ=255)
                                                                                                                    tttCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGa                                                                                                          >  5:260771/1‑75 (MQ=255)
                                                                                                                    tttCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCcttctt                                                    >  3:260231/1‑129 (MQ=255)
                                                                                                                    tttCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCcttctt                                                    <  4:260231/129‑1 (MQ=255)
                                                                                                                    tttCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCcgcg                                >  2:278424/1‑149 (MQ=255)
                                                                                                                      tcttcCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCcgcg                                <  6:66929/147‑1 (MQ=255)
                                                                                                                        ttcCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTcga                                                                                                                            >  2:232230/1‑53 (MQ=255)
                                                                                                                           cacgCCCACACCCTGCGGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGAGCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCt                          <  8:270907/145‑1 (MQ=255)
                                                                                                                            aGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGa                        >  2:259556/1‑149 (MQ=255)
                                                                                                                              gCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGTCCTGACt                      <  8:346666/149‑1 (MQ=255)
                                                                                                                                ccACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGa                                                                      <  6:7406/99‑1 (MQ=255)
                                                                                                                                ccACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGa                                                                      >  5:7406/1‑99 (MQ=255)
                                                                                                                                ccACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACttt                    <  4:354871/149‑1 (MQ=255)
                                                                                                                                 cacaCCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTc                   >  3:194264/1‑149 (MQ=255)
                                                                                                                                 cacaCCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTc                   <  6:347696/149‑1 (MQ=255)
                                                                                                                                   gacacTGGCGTACAGGCCGTACGGCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGc                            >  3:168933/5‑138 (MQ=255)
                                                                                                                                   gacacTGGCGTACAGGCCGTACGGCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGc                            <  4:168933/134‑1 (MQ=255)
                                                                                                                                    acCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGa                <  8:124169/149‑1 (MQ=255)
                                                                                                                                    acCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGa                <  6:82065/149‑1 (MQ=255)
                                                                                                                                     cccTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAg               <  3:361744/149‑1 (MQ=255)
                                                                                                                                      ccTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGt              >  4:130876/1‑149 (MQ=255)
                                                                                                                                        tggcGTACAGGCCGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATcc                                            <  2:212272/114‑1 (MQ=255)
                                                                                                                                        tggcGTACAGGCCGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATcc                                            >  1:212272/4‑117 (MQ=255)
                                                                                                                                             tACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAg         >  7:92095/1‑147 (MQ=255)
                                                                                                                                             tACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGga       <  7:270133/149‑1 (MQ=255)
                                                                                                                                                 gCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGAc    >  7:51304/1‑148 (MQ=255)
                                                                                                                                                  ccTGTACGCCAGAGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACAt  >  6:210822/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACAT  >  NC_002947/3148495‑3148789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑