Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,162,734 T→A 9.5% S34R (AGT→AGA)  PP_2777 → acyl carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,162,7340TA 9.5% 112.6 / 3.5 42S34R (AGT→AGA) PP_2777acyl carrier protein
Reads supporting (aligned to +/- strand):  ref base T (24/14);  new base A (2/2);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

GACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATG  >  NC_002947/3162592‑3162882
                                                                                                                                               |                                                                                                                                                    
gaagttATTCATTCAATGCGGATTTTGTAGTGAAACACTCAATGAATAATCCGTTGGACTTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTTCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACATCAGTAttgtt                                                                                                                                                <  8:251313/145‑1 (MQ=255)
 aCTTTATTCATTCAATGCGGCTTTTGTATGGACACAAGCACTGAATAATCCGTTGGAATTTGACAGCGTCATCAGCAGTACACAGTATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTAttgtt                                                                                                                                                <  8:246253/148‑1 (MQ=255)
   tttATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTTGACAGCGTCATAAGCAGTACAAAGGATATTATTTCGAAGTTTCTGGTGCTGGATAGGGG‑CGAAGTGAAATAGCAAAGCAGTATTGTTtac                                                                                                                                             >  1:99010/1‑149 (MQ=255)
   tttATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTgac                                                                                                                                             >  7:90221/1‑149 (MQ=255)
      attcattcAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTAAACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATA‑GGG‑CGACCTGAAAGAGCACAGCAGTATTTTTGAAGAcc                                                                                                                                         >  6:182785/1‑149 (MQ=255)
       ttcattcaATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGAcc                                                                                                                                         >  2:208138/1‑149 (MQ=255)
          attcaATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCa                                                                                                                                      >  4:234457/1‑149 (MQ=255)
           ttcaATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAg                                                                                                                                     >  6:359564/1‑149 (MQ=255)
                  cGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGAc                                                                                                                              <  3:71850/149‑1 (MQ=255)
                              ggAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGAACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACAtc                                                                                                                  >  5:11405/1‑149 (MQ=255)
                                      tCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGCCCt                                                                                                          >  2:232054/1‑149 (MQ=255)
                                         aTGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGGAAGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGa                                                                                                        <  1:119025/149‑1 (MQ=255)
                                          ttaataatCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGc                                                                                                      <  3:234457/147‑1 (MQ=255)
                                                 tCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGc                                                                                               >  1:359522/1‑149 (MQ=255)
                                                    gTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTgg                                                                                            <  4:52752/149‑1 (MQ=255)
                                                    gTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTgg                                                                                            >  4:56662/1‑149 (MQ=255)
                                                                           gcagTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTg                                                                     >  6:267452/1‑149 (MQ=255)
                                                                             agTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGCATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGcc                                                                   <  5:359564/149‑1 (MQ=255)
                                                                                      gATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAg                                                          >  6:334428/1‑149 (MQ=255)
                                                                                      gATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAg                                                          >  5:297170/1‑149 (MQ=255)
                                                                                      gATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTAAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAg                                                          >  1:211725/1‑149 (MQ=255)
                                                                                               gcgcAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTg                                                   <  6:188054/147‑1 (MQ=255)
                                                                                                       gctggtgctggATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTTAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCAcgcg                                          <  2:211725/148‑1 (MQ=255)
                                                                                                         tggtgctggATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCgg                                         >  7:214029/1‑147 (MQ=255)
                                                                                                            tgctggATAGGGG‑CGACGTGACAGAGCACAGCAGAATTGTTGACGACCTCAGCGCCGACTCGCTgg                                                                                                                       <  3:85214/66‑1 (MQ=255)
                                                                                                            tgctggATAGGGG‑CGACGTGACAGAGCACAGCAGAATTGTTGACGACCTCAGCGCCGACTCGCTgg                                                                                                                       <  3:80067/66‑1 (MQ=255)
                                                                                                            tgctggATAGGGG‑CGACGTGACAGAGCACAGCAGAATTGTTGACGACCTCAGCGCCGACTCGCTgg                                                                                                                       >  4:80067/1‑66 (MQ=255)
                                                                                                            tgctggATAGGGG‑CGACGTGACAGAGCACAGCAGAATTGTTGACGACCTCAGCGCCGACTCGCTgg                                                                                                                       >  4:85214/1‑66 (MQ=255)
                                                                                                             gctggATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCgg                                   >  3:70076/1‑149 (MQ=255)
                                                                                                              ctggATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGgt                                  >  8:117466/1‑149 (MQ=255)
                                                                                                               tggATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGgtg                                 >  4:276471/1‑149 (MQ=255)
                                                                                                                ggATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGgtgt                                >  4:316824/1‑149 (MQ=255)
                                                                                                                  aTAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTc                              <  1:208138/149‑1 (MQ=255)
                                                                                                                        g‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGTTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGAc                        >  2:155633/1‑149 (MQ=255)
                                                                                                                           gACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGc                      >  1:192109/1‑149 (MQ=255)
                                                                                                                              gTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCAc                   >  2:335044/1‑149 (MQ=255)
                                                                                                                                        acaGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGTTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTc         >  7:30449/1‑149 (MQ=255)
                                                                                                                                        acaGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAGACCCGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGGTTGTc         >  6:37339/1‑149 (MQ=255)
                                                                                                                                        acaGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTc         <  3:276471/149‑1 (MQ=255)
                                                                                                                                         cagcagTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCg        <  6:11405/149‑1 (MQ=255)
                                                                                                                                             agTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGaaaa    <  7:117466/149‑1 (MQ=255)
                                                                                                                                              gTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAAt   <  3:316824/149‑1 (MQ=255)
                                                                                                                                               tATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATg  >  5:170237/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCATCAGCAGTACACAGGATATTCTTGCGCAGTTGCTGGTGCTGGATAGGGG‑CGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATG  >  NC_002947/3162592‑3162882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑