Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,166,056	G→C	8.1%	G163A (GGC→GCC)	PP_2780 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,166,056	0	G	C	8.1%	189.4 / 6.9	62	G163A (GGC→GCC)	PP_2780	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (31/26);  new base C (3/2);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

GCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGA  >  NC_002947/3165916‑3166200                                                                                                                                              |                                                                                                                                                 gCAGGACATGCTGCTCAGCCTGGCGAATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCgg                                                                                                                                            >  5:288434/1‑147 (MQ=255)       cATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAg                                                                                                                                    <  5:365208/149‑1 (MQ=255)        aTGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAgg                                                                                                                                   >  3:136001/1‑149 (MQ=255)              ctCAGCCTGGCGAATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGt                                                                                                                             <  6:288434/149‑1 (MQ=255)               tCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCgg                                                                                                                              >  8:335341/1‑147 (MQ=255)               tCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCgg                                                                                                                              <  7:335341/147‑1 (MQ=255)                cAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGa                                                                                                                           >  4:110712/1‑149 (MQ=255)                 aGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGACCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGa                                                                                                                           <  1:274036/149‑1 (MQ=255)                 aGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGAt                                                                                                                          <  4:283788/149‑1 (MQ=255)                  gCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATc                                                                                                                         <  3:110712/149‑1 (MQ=255)                     tGGCGGATAACCCGGCGCAGGCGCCGTCGTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGAt                                                                                                                          >  1:214859/1‑145 (MQ=255)                     tGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGAGAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGAt                                                                                                                          <  2:214859/145‑1 (MQ=255)                       gCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAAt                                                                                                                      <  5:68850/147‑1 (MQ=255)                           ccaggacGGCGCCTGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTcag                                                                                                                 >  6:322662/7‑148 (MQ=255)                             aggacGGCGCCTGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTcagcag                                                                                                              >  5:252397/5‑149 (MQ=255)                                         gcgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCggg                                                                                                  <  2:26465/149‑1 (MQ=255)                                            ccGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGc                                                                                               >  4:55655/1‑149 (MQ=255)                                                       tCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCa                                                                                    >  3:146234/1‑149 (MQ=255)                                                         aaTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATc                                                                                  >  3:331399/1‑149 (MQ=255)                                                          aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCg                                                                                 >  3:365589/1‑149 (MQ=255)                                                          aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCg                                                                                 <  4:331399/149‑1 (MQ=255)                                                           ttGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGc                                                                                                                                           >  4:362785/1‑90 (MQ=255)                                                                 gcgcGACAGGTGCTGGTCGATCCTGACAGTTCACTGGGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCGGGTGATCAATGTCAGCCGCGCCTgc                                                                                                       <  1:316919/120‑1 (MQ=255)                                                                 gcgcGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATg                                                                           <  5:228066/148‑1 (MQ=255)                                                                   gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAt                                                                        >  7:245040/1‑149 (MQ=255)                                                                     gACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTc                                                                      >  2:305870/1‑149 (MQ=255)                                                                         ggCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATg                                                                           <  4:160084/140‑1 (MQ=255)                                                                         ggCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATg                                                                           >  3:160084/1‑140 (MQ=255)                                                                         ggCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTc                                                                      >  8:96940/1‑145 (MQ=255)                                                                         ggCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAga                                                                  <  7:96940/149‑1 (MQ=255)                                                                         ggCGCAGGTCGATCCTGACAGTGCAATGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGTTGATCAATGTCAGCAGCTCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAga                                                                  <  7:165910/149‑1 (MQ=255)                                                                            gcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                       <  4:238/141‑1 (MQ=255)                                                                            gcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                       >  3:238/1‑141 (MQ=255)                                                                            gcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgatg                                                                >  5:155511/1‑148 (MQ=255)                                                                                gTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCATGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCg                                                           >  6:241101/1‑149 (MQ=255)                                                                                    aTCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTcgc                                                       >  3:158443/1‑149 (MQ=255)                                                                                       cTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCgcggc                                                    >  1:199498/1‑149 (MQ=255)                                                                                        tGACAGTTCACACCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGCAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCgaggc                                                    >  6:255983/1‑148 (MQ=255)                                                                                          aCAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAg                                                 <  4:136001/149‑1 (MQ=255)                                                                                            aGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGt                                               >  6:371629/1‑149 (MQ=255)                                                                                            aGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCACGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGt                                               >  6:91040/1‑149 (MQ=255)                                                                                             gTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTc                                              <  5:322662/149‑1 (MQ=255)                                                                                                 aCTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGc                                          >  5:109150/1‑149 (MQ=255)                                                                                                     cGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTgg                                       <  7:21135/148‑1 (MQ=255)                                                                                                      gTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGCCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGc                                      >  1:265199/1‑148 (MQ=255)                                                                                                          gagCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATcc                                                            <  1:66106/122‑1 (MQ=255)                                                                                                          gagCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATcc                                                            >  2:66106/1‑122 (MQ=255)                                                                                                                        cTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCGGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGa                    <  6:252397/149‑1 (MQ=255)                                                                                                                             gcgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCgg               >  6:80226/1‑149 (MQ=255)                                                                                                                                ggTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGc            <  4:158443/149‑1 (MQ=255)                                                                                                                                cttcactGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGc            >  3:235023/7‑149 (MQ=255)                                                                                                                                 ttcactGCCGATGCCATCGGCAATGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCg                <  7:273423/139‑1 (MQ=255)                                                                                                                                 ttcactGCCGATGCCATCGGCAATGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCg                >  8:273423/6‑144 (MQ=255)                                                                                                                                 gTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCt           >  1:332525/1‑149 (MQ=255)                                                                                                                                    actGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATc                                                             >  5:268869/3‑96 (MQ=255)                                                                                                                                    actGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATc                                                             <  6:268869/94‑1 (MQ=255)                                                                                                                                       gCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGcc      <  4:365589/148‑1 (MQ=255)                                                                                                                                        ccGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGTTCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCt     <  5:371629/148‑1 (MQ=255)                                                                                                                                        ccGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCt     <  5:91040/148‑1 (MQ=255)                                                                                                                                        ccGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTg    >  5:326694/1‑149 (MQ=255)                                                                                                                                          gATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGa  <  6:326694/149‑1 (MQ=255)                                                                                                                                           aTGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGa  >  5:220948/1‑148 (MQ=255)                                                                                                                                              |                                                                                                                                                 GCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCG‑CCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGA  >  NC_002947/3165916‑3166200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑