Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,175,088 G→A 9.3% G130D (GGT→GAT)  PP_2787 → transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,175,0880GA 9.3% 115.5 / 2.6 43G130D (GGT→GAT) PP_2787transporter
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base A (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01

TGAACGCCCAGTTCGAGCTGTCCCAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGAAATGGCCTCGATG  >  NC_002947/3174944‑3175224
                                                                                                                                                |                                                                                                                                        
tGAACGCCCAGTTCGAGCTGTCCCAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGtttt                                                                                                                                      >  4:199475/1‑149 (MQ=255)
  aaCGCCCAGTTCGAGCTGTCCCAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTc                                                                                                                                    <  6:329547/149‑1 (MQ=255)
     gCCCAGTTCGAGCTGTCCCAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACCACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGcc                                                                                                                                 >  5:890/1‑148 (MQ=255)
     gCCCAGTTCGAGCTGTCACAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCa                                                                                                                                 <  1:304770/149‑1 (MQ=255)
              gAGCTGTCCCAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGAtt                                                                                                                         <  8:129254/148‑1 (MQ=255)
                  tGTCCCAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCt                                                                                                                    >  3:234598/1‑149 (MQ=255)
                       cAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTgccgc                                                                                                               >  5:364393/1‑149 (MQ=255)
                            cATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAg                                                                                                          >  1:154769/1‑149 (MQ=255)
                               actgcAGTTTATGCGGGCGATCGATGAGTACCTTGTCGAACTGCAGAGCATCGTCGGCCTGCACAGTGACGACGCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGTGTGTTTTTCGCAGAACGATTACGCTGCCGCCAAAgcg                                                                                                        <  8:281391/144‑1 (MQ=255)
                                  ccAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCt                                                                                                      <  8:176761/147‑1 (MQ=255)
                                  ccAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCt                                                                                                      >  7:176761/1‑147 (MQ=255)
                                  ccAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTgc                                                                                                    <  2:154769/149‑1 (MQ=255)
                                    aGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTgcgc                                                                                                  <  2:160476/149‑1 (MQ=255)
                                         aTGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAGGCGCTGCGCGACCa                                                                                             >  1:229864/1‑149 (MQ=255)
                                         aTGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCa                                                                                             >  8:269688/1‑149 (MQ=255)
                                            cTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATgg                                                                                          <  6:364393/149‑1 (MQ=255)
                                                 gAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGATTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTg                                                                                     >  5:131974/1‑149 (MQ=255)
                                                 gAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGATTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTg                                                                                     >  5:87583/1‑149 (MQ=255)
                                                  aaCGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGc                                                                                    <  4:343482/149‑1 (MQ=255)
                                                       tGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCgg                                                                                >  6:297360/1‑148 (MQ=255)
                                                           gACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTg                                                                           <  2:289367/149‑1 (MQ=255)
                                                            aCCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTgg                                                                          <  4:234598/149‑1 (MQ=255)
                                                                      aCTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCAt                                                                 >  6:363345/1‑148 (MQ=255)
                                                                      aCTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCAt                                                                 <  5:363345/148‑1 (MQ=255)
                                                                              gcATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTg                                                                           >  6:80850/1‑130 (MQ=255)
                                                                              gcATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTg                                                                           <  5:80850/130‑1 (MQ=255)
                                                                                aTCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGtt                                                      <  5:297360/149‑1 (MQ=255)
                                                                                    tcgGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCg                                                                                 <  2:161682/118‑1 (MQ=255)
                                                                                    tcgGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGttttt                                                   >  7:180385/1‑148 (MQ=255)
                                                                                      ggCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAg                                                <  7:269688/149‑1 (MQ=255)
                                                                                      ggCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGATTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGa                                                 <  6:131974/148‑1 (MQ=255)
                                                                                      ggCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGATTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGa                                                 <  6:87583/148‑1 (MQ=255)
                                                                                              caGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTg                                        <  3:199475/149‑1 (MQ=255)
                                                                                                        ccTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGAt                              >  7:339794/1‑149 (MQ=255)
                                                                                                                  aGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATc                    >  8:277439/1‑149 (MQ=255)
                                                                                                                           tGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGAAATg           <  4:85607/149‑1 (MQ=255)
                                                                                                                             gTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGa               <  7:307825/143‑1 (MQ=255)
                                                                                                                             gTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGa               >  8:307825/1‑143 (MQ=255)
                                                                                                                               gCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATg                                                                                           >  2:63382/1‑65 (MQ=255)
                                                                                                                                 aGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGAAATGGc         <  2:319402/145‑1 (MQ=255)
                                                                                                                                 aGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGAAATGGc         >  1:319402/1‑145 (MQ=255)
                                                                                                                                   aTCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGAAATGGCCTCGAt   >  5:288719/1‑149 (MQ=255)
                                                                                                                                     ccTCGCCGAGGGTTTTTCGCAGAACGATTACGCTCCCCCCAAAGCGCTGCGCGACCATGGGAATGCCCGGCACAGGGACACCCATGACCTGCAGTTTTTCAGATTTCTACCCGAGCCGAACAACCCGATCCAGGAAATGGCCTCGATg  >  5:278785/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                        
TGAACGCCCAGTTCGAGCTGTCCCAGGCCATACGCCAGTTGATGCTGGCGAACGATGAGGACCGTGTCGAACTGCAGCGCATCGTCGGCCTGCACAGTGACGACCCTGATGCCCAGGGTTTGCTGGTGCAGATCCTCGCCGAGGGTTTTTCGCAGAACGATTACGCTGCCGCCAAAGCGCTGCGCGACCATGGGATTGCCCGGCACTGGGACAGCCATGACCTGCAGTTTTTGAGCTTTCTTGCCGAGCGGATCAACCCGATCCAGGAAATGGCCTCGATG  >  NC_002947/3174944‑3175224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑