Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,199,099 G→A 7.8% T139I (ACC→ATC)  PP_2806 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,199,0990GA 7.8% 143.9 / 2.7 51T139I (ACC→ATC) PP_2806TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base A (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

ACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAACCAGTTGACAA  >  NC_002947/3198958‑3199243
                                                                                                                                             |                                                                                                                                                
aCTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCtt                                                                                                                                           >  4:107126/1‑149 (MQ=255)
   gCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCAc                                                                                                                                        >  7:221613/1‑149 (MQ=255)
      tGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGAt                                                                                                                                     >  6:368800/1‑149 (MQ=255)
       ggCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAAAAGATTGATCAGCAAGCGGCTGATAGTGGTAAGTGCTGCATGGTCAAGTGAGCCGTCGGTGACCTTCACGaag                                                                                                                                    >  6:17287/1‑147 (MQ=255)
          aGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGcc                                                                                                                                 <  4:137419/149‑1 (MQ=255)
                    ggtctcggCCTCCAGGCGTGCACGGTCGCCGTGAGCTGACCAGGCGAGAATCAGGCCGTCGAACAGATTGATCAGCAAGCGTCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTTTTGGGc                                                                                                                         <  5:13970/147‑1 (MQ=255)
                    ggtctcggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGc                                                                                                                         <  1:112185/147‑1 (MQ=255)
                        tcggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGc                                                                                                                                  <  8:209485/134‑1 (MQ=255)
                        tcggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGc                                                                                                                                  >  7:209485/1‑134 (MQ=255)
                          ggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTc                                                                                                                   >  3:330772/1‑147 (MQ=255)
                          ggCCTCCAGGCGTGCACGGGCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGa                                                                                                                 <  4:240777/149‑1 (MQ=255)
                                cAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGaaa                                                                                                           >  2:46810/1‑149 (MQ=255)
                                 aGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAAc                                                                                                          <  8:276698/149‑1 (MQ=255)
                                   gCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTTACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACgg                                                                                                        <  8:92539/149‑1 (MQ=255)
                                        cACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCa                                                                                                      >  1:331702/1‑146 (MQ=255)
                                          cGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGAt                                                                                                 >  3:288115/1‑149 (MQ=255)
                                                ccGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGt                                                                                           >  4:290898/1‑149 (MQ=255)
                                                            cAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCgg                                                                               >  2:214098/1‑149 (MQ=255)
                                                               gCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGgtt                                                                            <  6:10827/149‑1 (MQ=255)
                                                                         ggCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAgg                                                                  <  3:290898/149‑1 (MQ=255)
                                                                         ggCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAgg                                                                  >  1:6209/1‑149 (MQ=255)
                                                                              tCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCATTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCa                                                             <  5:17287/149‑1 (MQ=255)
                                                                              tCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCa                                                             <  2:157571/149‑1 (MQ=255)
                                                                                gAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAg                                                            <  1:46810/148‑1 (MQ=255)
                                                                                    aGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCaaaa                                                       <  5:368800/149‑1 (MQ=255)
                                                                                      aTTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCa                                                             <  7:63319/141‑1 (MQ=255)
                                                                                      aTTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTACAGTGCGCCGTCGGTGACCTTCACGATGGCCTGATGGGACGCTTCTAGGGAAACTCAATACATCTTGGTTGCCATCTCCGGATTGTTGCGCAGGTTCCa                                                             >  8:63319/1‑141 (MQ=255)
                                                                                         gATCAGCAAGCGGCTGATAGTGGTGAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGtt                                                  >  4:256315/1‑149 (MQ=255)
                                                                                          aTCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGtt                                                  <  3:107126/148‑1 (MQ=255)
                                                                                           tCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTc                                                 >  3:369232/1‑148 (MQ=255)
                                                                                                aaGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGATGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACa                                                     <  5:197762/139‑1 (MQ=255)
                                                                                                aaGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGATGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACa                                                     <  5:202792/139‑1 (MQ=255)
                                                                                                aaGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGATGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACa                                                     >  6:202792/1‑139 (MQ=255)
                                                                                                aaGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGATGACCTTCACGATGGCATGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACa                                                     >  6:197762/1‑139 (MQ=255)
                                                                                                  gCGGCTGATAGTGGTCAGTGCTGACTGGTACAGTGCGCCGTCGGTGACCTTAACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATGTTGGTTGCAATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTAGAACTGCg                                         >  3:84922/1‑149 (MQ=255)
                                                                                                    ggCTGAAAGTGGTCAGTGCTGCCTGCTCCAGTGCGCCGTCCGTGACCTTCACGATTGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCGGTCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTg                                       >  6:254604/1‑149 (MQ=255)
                                                                                                      cTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGt                                     <  4:288115/149‑1 (MQ=255)
                                                                                                      cTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGt                                     <  6:329484/149‑1 (MQ=255)
                                                                                                         aTAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCa                                                                    >  3:270927/1‑115 (MQ=255)
                                                                                                         aTAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCa                                                                    <  4:270927/115‑1 (MQ=255)
                                                                                                         aTAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTc                                    <  2:331702/147‑1 (MQ=255)
                                                                                                             tGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCAtc                              >  6:352639/1‑149 (MQ=255)
                                                                                                               gTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTTGTCGCCAtct                             >  5:183426/1‑148 (MQ=255)
                                                                                                                   gTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGa                        >  3:301391/1‑149 (MQ=255)
                                                                                                                         ccTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGc                   <  6:183426/148‑1 (MQ=255)
                                                                                                                              tCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGaa             >  3:15948/1‑149 (MQ=255)
                                                                                                                                cAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAAc            <  5:352639/148‑1 (MQ=255)
                                                                                                                                cAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAAc            >  3:310690/1‑148 (MQ=255)
                                                                                                                                  gTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAACCAg         >  3:354401/1‑149 (MQ=255)
                                                                                                                                         gTCGGTGACGTTGTCGATGGCCTGTTGTGCCGATTCGAGTGAAACGGCATATATCTTGGTTGCCATCTCCGGGTTGTTTCGCAGGTTCCAGCAAATCAGTTCTATCTGCGTGGTCGCCATCTCAGTATGAGCGGTGAACCATTTGACaa  <  4:310690/149‑1 (MQ=255)
                                                                                                                                         gTCGGTGACCTTCACGATGGCCTGTTGGGCCTCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAACCAGTTGACaa  <  3:256315/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
ACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAACCAGTTGACAA  >  NC_002947/3198958‑3199243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑