Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,209,677 T→A 7.5% F301I (TTC→ATC)  PP_2813 → BNR domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,209,6770TA 7.5% 156.1 / 3.2 53F301I (TTC→ATC) PP_2813BNR domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base A (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

AGCAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAC  >  NC_002947/3209530‑3209825
                                                                                                                                                   |                                                                                                                                                    
aGCAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCgttgtt                                                                                                                                                     <  1:251692/149‑1 (MQ=255)
  cAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTc                                                                                                                                                    >  3:28268/1‑148 (MQ=255)
      ggCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGc                                                                                                                                               >  4:362633/1‑149 (MQ=255)
          ctGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGGCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGAACAACGCCgcc                                                                                                                                           <  8:295113/149‑3 (MQ=255)
             tCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAg                                                                                                                                          <  3:362633/147‑1 (MQ=255)
             tCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGgt                                                                                                                                        <  1:325985/149‑1 (MQ=255)
               aCTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGgtg                                                                                                                                       >  3:29146/1‑148 (MQ=255)
                    cGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCt                                                                                                                                  <  4:29146/148‑1 (MQ=255)
                     gTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACGGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTg                                                                                                                                 >  7:144381/1‑148 (MQ=255)
                           gTAGGCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCTGCGATGGCGGGCACAGTTTGCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGtt                                                                                                                          <  3:315751/149‑1 (MQ=255)
                              gCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGtttt                                                                                                                        >  8:248613/1‑148 (MQ=255)
                                cGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCAc                                                                                                                     <  7:248613/149‑1 (MQ=255)
                                            gCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTaa                                                                                                         >  5:160996/1‑149 (MQ=255)
                                                gCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATg                                                                                                       <  6:256297/147‑1 (MQ=255)
                                                  ggTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTAACGCCGAGGGGTAATGccc                                                                                                    >  6:56681/1‑148 (MQ=255)
                                                       ggCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAg                                                                                              <  4:128690/149‑1 (MQ=255)
                                                                 gCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAggg                                                                                    >  6:268513/1‑149 (MQ=255)
                                                                 gCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAggg                                                                                    <  5:277555/149‑1 (MQ=255)
                                                                      gCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGc                                                                               >  5:57752/1‑149 (MQ=255)
                                                                      gCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGc                                                                               >  5:190106/1‑149 (MQ=255)
                                                                        gTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCa                                                                             >  2:166465/1‑149 (MQ=255)
                                                                         tGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCAc                                                                            >  5:13515/1‑149 (MQ=255)
                                                                          gCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACt                                                                           >  3:216240/1‑149 (MQ=255)
                                                                                      tGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGc                                                                >  6:383293/1‑148 (MQ=255)
                                                                                        gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCa                                                              <  1:166465/148‑1 (MQ=255)
                                                                                        gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCa                                                              <  8:144381/148‑1 (MQ=255)
                                                                                        gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                             <  4:28268/149‑1 (MQ=255)
                                                                                         cGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAgg                                                            <  5:228881/149‑1 (MQ=255)
                                                                                               caGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGc                                                      <  6:13515/149‑1 (MQ=255)
                                                                                                 gTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCggtt                                                    >  3:68132/1‑146 (MQ=255)
                                                                                                  tttCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCaaa                                                   >  7:301186/1‑149 (MQ=255)
                                                                                                  tttCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCaaa                                                   >  5:234348/1‑149 (MQ=255)
                                                                                                       aGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGAt                                              >  3:89124/1‑149 (MQ=255)
                                                                                                             cGCAGACCTCGGATGGCGTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCTCGCCGATGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGGAAACCGATgcctgg                                        <  3:242011/149‑1 (MQ=255)
                                                                                                             cGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGGTTTGCGATgcctgg                                        <  6:57752/149‑1 (MQ=255)
                                                                                                               cAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGGTTTGCGATgcctggct                                      <  4:68132/149‑2 (MQ=255)
                                                                                                                    cgcgGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCg                                                       <  1:337722/127‑1 (MQ=255)
                                                                                                                    cgcgGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCg                                                       >  2:337722/1‑127 (MQ=255)
                                                                                                                      cgGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTc                               <  4:89124/149‑1 (MQ=255)
                                                                                                                        gATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCtt                             <  4:216240/149‑1 (MQ=255)
                                                                                                                            gCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCCCGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCt                         >  5:262814/1‑149 (MQ=255)
                                                                                                                             ccTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTg                        <  5:383293/149‑1 (MQ=255)
                                                                                                                             ccTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTg                        >  2:11258/1‑149 (MQ=255)
                                                                                                                               tttCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGAt                                              <  8:253763/125‑1 (MQ=255)
                                                                                                                               tttCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGAt                                              >  7:253763/1‑125 (MQ=255)
                                                                                                                                   aCTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCt                         >  1:114487/1‑142 (MQ=255)
                                                                                                                                   aCTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCt                         <  2:114487/142‑1 (MQ=255)
                                                                                                                                    cTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAAt                 <  6:190106/149‑1 (MQ=255)
                                                                                                                                       ccttcggcgTTGAACAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGa                                               <  3:180664/112‑1 (MQ=255)
                                                                                                                                       ccttcggcgTTGAACAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGa                                               >  4:180664/5‑116 (MQ=255)
                                                                                                                                        cggcggCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggt             <  6:160996/149‑1 (MQ=255)
                                                                                                                                         ttcggcgTTGAACAACGCCGAAGGTGTACCTGTATTGTTTTCACGACGAGGGGTAAAGCCCTACCAGGAGTGAAGGGATGGACACTCGCTCAGAAGCCAGGAATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggtg            >  7:318500/3‑149 (MQ=255)
                                                                                                                                            ggcgTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTc         >  1:48676/1‑149 (MQ=255)
                                                                                                                                            ggcgTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAAGGGGTGGTc         >  4:227266/1‑149 (MQ=255)
                                                                                                                                                   ttCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAc  >  1:261287/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGCAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAC  >  NC_002947/3209530‑3209825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑