Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,275,871 A→T 11.2% intergenic (+26/‑116) PP_5540 → / → PP_2874 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,275,8710AT11.2% 120.5 / 5.2 51intergenic (+26/‑116)PP_5540/PP_2874hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base T (2/4);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGCATTTCGCCTGGGTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAGGGAAGAAACCTATT  >  NC_002947/3275725‑3276017
                                                                                                                                                  |                                                                                                                                                  
ggcggcATTTCGCCTGGGTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAAc                                                                                                                                                  >  1:247250/1‑149 (MQ=255)
       tttCGCCTGGGTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCgggg                                                                                                                                           >  2:311905/1‑149 (MQ=255)
         tCGCCTTGGTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCGTTGGGCGGGGt                                                                                                                                          <  3:219469/148‑1 (MQ=255)
          cgtcTGGGTTTCGCACACATGACGCTTGATGATTCAGAACAGCTTCGGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACAGATCCCGCCCACCGGCGGGGtt                                                                                                                                         <  2:275984/145‑1 (MQ=255)
            ccTGGGTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCGTTGGGCGGGGtgtgt                                                                                                                                      >  3:354757/1‑145 (MQ=255)
            ccTGGGTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCGTTGGGCGGGGtgtgt                                                                                                                                      <  4:354757/149‑5 (MQ=255)
                ggTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTAtt                                                                                                                                   <  5:379016/148‑1 (MQ=255)
                  tttCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGcc                                                                                                                                <  7:207492/149‑1 (MQ=255)
                       cccACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGACATTCg                                                                                                                           >  8:177201/1‑149 (MQ=255)
                        ccACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGt                                                                                                                          >  6:318643/1‑149 (MQ=255)
                         cacaTGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGtt                                                                                                                         >  6:101060/1‑149 (MQ=255)
                                 gCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGcc                                                                                                                 >  2:233461/1‑149 (MQ=255)
                                        gatTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCACGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATgg                                                                                                           >  4:209088/1‑148 (MQ=255)
                                        gatTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGAACCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTAAATGCCATACGTTGATCCGCCGCAtcg                                                                                                           >  7:68179/1‑146 (MQ=255)
                                                    cTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCGTTGGGCGGGGt                                                                                                                                          <  2:12501/105‑1 (MQ=255)
                                                    cTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCGTTGGGCGGGGt                                                                                                                                          >  1:12501/1‑105 (MQ=255)
                                                    cTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAg                                                                                              >  2:202831/1‑149 (MQ=255)
                                                    cTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAg                                                                                              >  1:80167/1‑149 (MQ=255)
                                                       cAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATc                                                                                                                     <  5:261712/123‑1 (MQ=255)
                                                       cAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATc                                                                                                                     >  6:261712/1‑123 (MQ=255)
                                                         gCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCGCACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTc                                                                                         <  5:183770/149‑1 (MQ=255)
                                                              cacaTTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCg                                                                                     <  2:365529/148‑1 (MQ=255)
                                                               acaTTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCTTTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGa                                                                                   >  3:337254/1‑149 (MQ=255)
                                                                      cagcgcagcAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCg                                                                            >  7:323838/1‑149 (MQ=255)
                                                                        gcgcagcAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGc                                                                          <  7:345271/149‑1 (MQ=255)
                                                                          gcagcaAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCt                                                                        <  8:323838/149‑1 (MQ=255)
                                                                            agcaAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCtgt                                                                      >  6:269827/1‑149 (MQ=255)
                                                                             gcaAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCtgtg                                                                     <  2:247250/149‑1 (MQ=255)
                                                                              caAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTgg                                                                    >  3:181852/1‑149 (MQ=255)
                                                                                    aGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCt                                                              <  7:177201/149‑1 (MQ=255)
                                                                                    aGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCt                                                              <  4:181852/149‑1 (MQ=255)
                                                                                     ggCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTg                                                             >  3:319920/1‑149 (MQ=255)
                                                                                        cGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCaa                                                                                                                                                   <  2:372960/60‑1 (MQ=255)
                                                                                        cGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCaa                                                                                                                                                   >  1:372960/1‑60 (MQ=255)
                                                                                                  cgcgGGTAGCGCTCATGTAGTGATCCGGCTTCTGCACACTCCCCGACCATCGGCGGGGTTTTTATTGCCTTTCGTTGATCCGCCTCATGGTATTGCCCGGTTGCTCTATCCGGATGGCCCTGCATTTGGTTTTCTTACGCGTCATTGCa                                                <  4:337254/149‑1 (MQ=255)
                                                                                                          ccGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACgga                                        <  5:318643/149‑1 (MQ=255)
                                                                                                            gCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCAGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACggag                                       >  1:208203/1‑148 (MQ=255)
                                                                                                                  gaagTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGaa                                >  2:329774/1‑149 (MQ=255)
                                                                                                                   aagTGATCCCGGGTCTGCACACACCCGGCCATTCGTCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAAt                               <  8:68179/149‑1 (MQ=255)
                                                                                                                         tCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCAt                         >  3:103678/1‑149 (MQ=255)
                                                                                                                          cccGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATg                        <  3:202123/149‑1 (MQ=255)
                                                                                                                           ccGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGc                       <  5:297409/149‑1 (MQ=255)
                                                                                                                           ccGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGc                       <  3:209088/149‑1 (MQ=255)
                                                                                                                                    cacacaCCCCTCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAggg              <  5:269827/149‑1 (MQ=255)
                                                                                                                                     acacacCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTCGCGCTCGCCGGATGGCCCGGCCGGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGGTCATGCGCGTCAGGga             >  4:260884/1‑149 (MQ=255)
                                                                                                                                      cacacCCCGCCCCACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGGTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAGGgaa            <  4:319920/149‑1 (MQ=255)
                                                                                                                                      cacacCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAGGgaa            <  4:103678/149‑1 (MQ=255)
                                                                                                                                       acacCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAggg              >  7:195411/1‑146 (MQ=255)
                                                                                                                                           cccGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCtgtg                                                                     >  2:139671/1‑87 (MQ=255)
                                                                                                                                           cccGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAggg              <  7:174943/142‑1 (MQ=255)
                                                                                                                                           cccGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAggg              >  8:174943/1‑142 (MQ=255)
                                                                                                                                               cccAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAGGGAAGAAACCTAt   <  5:101060/149‑1 (MQ=255)
                                                                                                                                                ccAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGATTGCTCATGCGAGTCAGGGAAGAAAGCTAtt  <  2:208203/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCGGCATTTCGCCTGGGTTTCGCCCACATGACGCTGGATGATTCCGAACAGCTCCAGCCTGCACATTTGCAGCGCAGCAAAAAAGGCCGCTTCATGCCGCGGGTACCGCTCAAGAAGTGATCCCGCGTCTGCACACACCCCGCCCAACGGCGGGGTTTTTATTGCCATTCGTTGATCCGCCTCATGGTATTGCCCGGTAGCGCTCGCCGGATGGCCCGGCCTGTGGTTTTCTGACGCGGCATTCCAGCAACGGAGGATTGAATGCTCATGCGAGTCAGGGAAGAAACCTATT  >  NC_002947/3275725‑3276017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑