Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,276,756 G→A 8.0% intergenic (‑61/+222) PP_2875 ← / ← ampC hypothetical protein/beta‑lactamase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,276,7560GA 8.0% 140.7 / 2.5 50intergenic (‑61/+222)PP_2875/ampChypothetical protein/beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base A (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

TCCTTGCGCACGGCGAACAGCTGGGCCAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTCATCATGCCCAAGCGCGATGAA  >  NC_002947/3276613‑3276903
                                                                                                                                               |                                                                                                                                                   
tCCTTGCGCACGGCGAACAGCTGGGCCAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAgcagca                                                                                                                                                >  1:368039/1‑149 (MQ=255)
  cTTGCGCACGGCGAACAGCTGGGCCCATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATAACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATc                                                                                                                                              >  2:178013/1‑149 (MQ=255)
       gcACGGCGAACAGCTGGGCCAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCaa                                                                                                                                          >  6:173123/1‑148 (MQ=255)
         aCGGCGAACAGCTGGGCCAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAg                                                                                                                                       >  4:309892/1‑149 (MQ=255)
             cGAACAGCTGGGCCAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGc                                                                                                                                   <  4:347250/149‑1 (MQ=255)
             cGAACAGCTGGGCCAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGc                                                                                                                                   >  2:244650/1‑149 (MQ=255)
                         ccAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTg                                                                                                                       >  4:324651/1‑149 (MQ=255)
                                gTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTg                                                                                                                <  1:36350/149‑1 (MQ=255)
                                   gATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGATGGAGGTATCACGGAGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAAAAGGGGCTTTCTCACGCTGCCTGTTGGa                                                                                                              >  6:374342/1‑148 (MQ=255)
                                        tCGGATTGTTGTTCAAGTTCATCATAAAGCGCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGttt                                                                                                        >  1:31968/1‑149 (MQ=255)
                                        tCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTAcagcag                                                                                                                                                  <  4:332343/107‑1 (MQ=255)
                                        tCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGttt                                                                                                        >  7:59481/1‑149 (MQ=255)
                                              tgttgtTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCctc                                                                                                   >  4:302127/1‑148 (MQ=255)
                                                   ttCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACg                                                                                                                          <  4:82787/120‑1 (MQ=255)
                                                     cAAGTTCATCATAAAGCCACCATTACCCATTCATATGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAACAGCATAGCAACAGGGGCTTTCTAACTCTGCCTTGTGGACAGTTTCCCTCTCCaagga                                                                                           >  4:281132/1‑149 (MQ=255)
                                                       aGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCacgga                                                                                           <  2:104584/147‑1 (MQ=255)
                                                       aGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCacgga                                                                                           >  1:104584/1‑147 (MQ=255)
                                                        gTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCacggacgg                                                                                        >  6:237234/1‑149 (MQ=255)
                                                         ttCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCTCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGc                                                                                       <  2:224849/149‑1 (MQ=255)
                                                         ttCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGc                                                                                       <  4:157953/149‑1 (MQ=255)
                                                            atcatAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCt                                                                                     <  1:70417/148‑1 (MQ=255)
                                                             tcaaacaGCCCCCAATACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGc                                                                                   <  3:266751/143‑1 (MQ=255)
                                                              catAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCg                                                                                  >  5:65754/1‑149 (MQ=255)
                                                               atAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGt                                                                                 <  1:244650/149‑1 (MQ=255)
                                                                tAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCgcg                                                                                >  5:207310/1‑147 (MQ=255)
                                                                  aaGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTa                                                                               <  3:309892/148‑1 (MQ=255)
                                                                       cccATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAACAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCgcgc                                                                         >  5:250132/1‑149 (MQ=255)
                                                                        ccATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTg                                                                                    >  2:37637/1‑137 (MQ=255)
                                                                        ccATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTg                                                                                    <  1:37637/137‑1 (MQ=255)
                                                                          aTTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAgg                                                                      <  5:237234/149‑1 (MQ=255)
                                                                                 aTTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCCCGCAGGACGTCTg                                                               >  4:190592/1‑149 (MQ=255)
                                                                                     aTAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGaa                                                            <  1:178013/148‑1 (MQ=255)
                                                                                                    aGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCt                                            >  3:335767/1‑149 (MQ=255)
                                                                                                    aGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCt                                            >  4:85320/1‑149 (MQ=255)
                                                                                                      ttGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGTGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTcc                                          >  2:112992/1‑149 (MQ=255)
                                                                                                      ttGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTcc                                          <  3:302127/149‑1 (MQ=255)
                                                                                                               tCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCTCGATCggg                                  <  5:2410/148‑1 (MQ=255)
                                                                                                               tCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCggg                                  <  5:173123/148‑1 (MQ=255)
                                                                                                               tCACGGTGTAGCGCACTACCAAGGCTGCTACAACAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCggg                                  <  6:250132/148‑1 (MQ=255)
                                                                                                                    gtgtAGCGCACTACCAAGGCTGCTACAACAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACg                            <  3:281132/149‑1 (MQ=255)
                                                                                                                              cTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTcatcat                  <  8:59481/149‑1 (MQ=255)
                                                                                                                              cTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTcatcat                  <  4:335767/149‑1 (MQ=255)
                                                                                                                               tACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACAccc                                                       >  2:164159/1‑111 (MQ=255)
                                                                                                                               tACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACAccc                                                       <  1:164159/111‑1 (MQ=255)
                                                                                                                               tACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTCATCATg                 >  5:36006/1‑149 (MQ=255)
                                                                                                                                 ccAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCgg                                   >  7:324291/1‑129 (MQ=255)
                                                                                                                                 ccAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCgg                                   <  8:324291/129‑1 (MQ=255)
                                                                                                                                  cAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTAACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCAGTCTGGAACACCCTTGCCACCGCTACCGCTCGTGGAGACGGCTTCATCATGcg               >  4:233353/1‑147 (MQ=255)
                                                                                                                                   aaGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTCATCATGccc              >  6:220484/1‑148 (MQ=255)
                                                                                                                                    aGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGc                                             >  4:29662/1‑116 (MQ=255)
                                                                                                                                    aGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGc                                             <  3:29662/116‑1 (MQ=255)
                                                                                                                                      gctgctACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTCATCATGCCCAAgc          >  8:253893/1‑149 (MQ=255)
                                                                                                                                              agcagcATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTCATCATGCCCAAGCGCGATGaa  >  4:115666/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TCCTTGCGCACGGCGAACAGCTGGGCCAATTGGTCGATAGTCGGATTGTTGTTCAAGTTCATCATAAAGCCCCCATTACCCATTCATAGGTGATTTTCACAGTTGGCGCTATCACGGTGTAGCGCACTACCAAGGCTGCTACAGCAGCATCGCAACAGGGGCTTTCTCACGCTGCCTTTTGGACAGTTTCCCTCTCCACGGACGGCCTGCGTTACCGCGCAGGCCGTCTGGAACACCCTTGCCACCGCTCCCGATCGGGGAGACGGCTTCATCATGCCCAAGCGCGATGAA  >  NC_002947/3276613‑3276903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑