Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,312,687 G→T 8.2% intergenic (+28/‑134) PP_2912 → / → hemB hypothetical protein/5‑aminolevulinate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,312,6870GT 8.2% 147.1 / 2.0 50intergenic (+28/‑134)PP_2912/hemBhypothetical protein/5‑aminolevulinate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (25/20);  new base T (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CGGGGTGATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTC  >  NC_002947/3312539‑3312835
                                                                                                                                                    |                                                                                                                                                    
cGGGGTGATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCgggg                                                                                                                                                      <  5:60708/149‑1 (MQ=255)
      gATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTAccc                                                                                                                                                <  2:356705/149‑1 (MQ=255)
           gTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGaa                                                                                                                                           <  4:267551/149‑1 (MQ=255)
              agaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGTAACCCCGCGAagag                                                                                                                                        <  5:233407/149‑1 (MQ=255)
              agaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAaga                                                                                                                                         <  6:163480/148‑1 (MQ=255)
               gaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAgg                                                                                                                                       >  6:355230/1‑149 (MQ=255)
                    aCGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTAGCGGGGTTACCCGCGCAGAGGCCacc                                                                                                                                  >  6:201349/1‑147 (MQ=255)
                     cgccgcCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGAACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAaca                                                                                                                                 <  3:235926/149‑1 (MQ=255)
                           ccGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGc                                                                                                                            >  7:64394/1‑148 (MQ=255)
                                 ggACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAgg                                                                                                                             <  4:316213/141‑1 (MQ=255)
                                 ggACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAgg                                                                                                                             >  3:316213/1‑141 (MQ=255)
                                  gacgacGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACg                                                                                                                    <  8:64394/149‑1 (MQ=255)
                                        gCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACCGGCAAACCACGGCCGtt                                                                                                              >  6:148840/1‑149 (MQ=255)
                                        gCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGtt                                                                                                              <  4:121068/149‑1 (MQ=255)
                                            gtcgCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCCAACCACGGCCGTTGCCa                                                                                                          >  1:161343/1‑149 (MQ=255)
                                              cgCCGTGCAGCAGGCGCCGTGGCCTCGCTTGGGCCGGCCAAGGCTGTGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCTATGAGGCCAACACAGGCAAACCACGGCCGTTGCCAtt                                                                                                        <  2:161343/149‑1 (MQ=255)
                                               gCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCAtt                                                                                                        >  4:136754/1‑148 (MQ=255)
                                                             gccgaggccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAACCCACGGCCGTTGCCATTGACGCAAGACACAtt                                                                                         >  8:270816/1‑149 (MQ=255)
                                                                      gagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCagt                                                                                >  4:147429/1‑147 (MQ=255)
                                                                             ggccggccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCtt                                                                         >  5:77641/1‑149 (MQ=255)
                                                                              gccggccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCttt                                                                        >  3:270830/1‑149 (MQ=255)
                                                                                   ccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGTAACCCCGCGAag                                                                                                                                          >  4:45352/1‑78 (MQ=38)
                                                                                   ccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGTAACCCCGCGAag                                                                                                                                          <  3:45352/78‑1 (MQ=38)
                                                                                    cAAGGCAGTGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCGGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTg                                                                  <  3:12333/149‑1 (MQ=255)
                                                                                    cAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTg                                                                  <  2:78911/149‑1 (MQ=255)
                                                                                    cAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTAGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTg                                                                  >  1:6206/1‑149 (MQ=255)
                                                                                       ggCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGgcg                                                               >  5:19634/1‑149 (MQ=255)
                                                                                          agagGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGgcgcgc                                                            <  7:103871/149‑1 (MQ=255)
                                                                                               aggTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAg                                                       <  4:270830/149‑1 (MQ=255)
                                                                                                 gTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGc                                                     <  5:201349/149‑1 (MQ=255)
                                                                                                      gccgccGTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCTGGCTTACCTGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATAGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGTCGCGCTACAGGCATAAg                                                <  8:158891/149‑1 (MQ=255)
                                                                                                      gccgccCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCAt                                                                                 <  5:3281/116‑1 (MQ=255)
                                                                                                      gccgccCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCAt                                                                                 >  6:3281/1‑116 (MQ=255)
                                                                                                                    agtagtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACAATGTCGGCATTTAGCAATTTAACTGGAGCGCGACACGCATTATGTATACGCAGtta                                   >  8:355502/1‑147 (MQ=255)
                                                                                                                      tagtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCaaa                                >  1:252542/1‑149 (MQ=255)
                                                                                                                      tagtTTATTCCTGTACCGCCTCTTCGCGGGGGTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACGGGCGCGCGACAGGCTTAAGGTTTACGCCGTTCaaa                                >  5:179911/1‑149 (MQ=255)
                                                                                                                       agtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCaaa                                <  3:136754/148‑1 (MQ=255)
                                                                                                                        gtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAAtt                              >  5:111477/1‑149 (MQ=255)
                                                                                                                             ttCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTg                         <  7:355502/149‑1 (MQ=255)
                                                                                                                              tCCTGTACCGCCAATTAGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTTACGCAAGACACATTCTAGTCATTTAACCTTTTAACTGGCGCGCCACAGGCATAAGGTATACGCCCTTCAAAATACCTg                         >  8:1861/1‑148 (MQ=255)
                                                                                                                                cTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTAGTCATTTAGCCTTTTAACTGGCGCGCGACAGGCATAAGGTATAAGCCGTTCAAATTACCTGGAg                      >  6:287627/1‑149 (MQ=255)
                                                                                                                                    aCCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGt                  >  2:212165/1‑149 (MQ=255)
                                                                                                                                     ccGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGt                  <  7:1861/148‑1 (MQ=255)
                                                                                                                                       gCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTc                             >  5:338978/1‑135 (MQ=255)
                                                                                                                                       gCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTc                             <  6:338978/135‑1 (MQ=255)
                                                                                                                                        ccTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATg              >  4:247327/1‑149 (MQ=255)
                                                                                                                                         ctctTCGCGGGTAACCCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCCCTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGt             >  3:224567/1‑149 (MQ=255)
                                                                                                                                          tctTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTc            >  4:3375/1‑149 (MQ=255)
                                                                                                                                            ttCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTcc           >  1:182753/1‑148 (MQ=255)
                                                                                                                                              cgcgGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTTCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTcc           <  6:111477/146‑1 (MQ=255)
                                                                                                                                              cgcgGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTccaa         <  2:41317/148‑1 (MQ=255)
                                                                                                                                                    gTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTc  <  6:77641/149‑1 (MQ=255)
                                                                                                                                                    gTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTc  <  3:3375/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGGGTGATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTC  >  NC_002947/3312539‑3312835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑