Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,312,690 A→C 7.8% intergenic (+31/‑131) PP_2912 → / → hemB hypothetical protein/5‑aminolevulinate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,312,6900AC 7.8% 121.8 / 4.7 52intergenic (+31/‑131)PP_2912/hemBhypothetical protein/5‑aminolevulinate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (25/23);  new base C (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01

GATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTC  >  NC_002947/3312545‑3312835
                                                                                                                                                 |                                                                                                                                                 
gATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTAccc                                                                                                                                                <  2:356705/149‑1 (MQ=255)
     gTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGaa                                                                                                                                           <  4:267551/149‑1 (MQ=255)
        agaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGTAACCCCGCGAagag                                                                                                                                        <  5:233407/149‑1 (MQ=255)
        agaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAaga                                                                                                                                         <  6:163480/148‑1 (MQ=255)
         gaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAgg                                                                                                                                       >  6:355230/1‑149 (MQ=255)
              aCGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTAGCGGGGTTACCCGCGCAGAGGCCacc                                                                                                                                  >  6:201349/1‑147 (MQ=255)
               cgccgcCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGAACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAaca                                                                                                                                 <  3:235926/149‑1 (MQ=255)
                     ccGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGc                                                                                                                            >  7:64394/1‑148 (MQ=255)
                           ggACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAgg                                                                                                                             <  4:316213/141‑1 (MQ=255)
                           ggACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAgg                                                                                                                             >  3:316213/1‑141 (MQ=255)
                            gacgacGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACg                                                                                                                    <  8:64394/149‑1 (MQ=255)
                                  gCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACCGGCAAACCACGGCCGtt                                                                                                              >  6:148840/1‑149 (MQ=255)
                                  gCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGtt                                                                                                              <  4:121068/149‑1 (MQ=255)
                                      gtcgCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCCAACCACGGCCGTTGCCa                                                                                                          >  1:161343/1‑149 (MQ=255)
                                        cgCCGTGCAGCAGGCGCCGTGGCCTCGCTTGGGCCGGCCAAGGCTGTGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCTATGAGGCCAACACAGGCAAACCACGGCCGTTGCCAtt                                                                                                        <  2:161343/149‑1 (MQ=255)
                                         gCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCAtt                                                                                                        >  4:136754/1‑148 (MQ=255)
                                                       gccgaggccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAACCCACGGCCGTTGCCATTGACGCAAGACACAtt                                                                                         >  8:270816/1‑149 (MQ=255)
                                                                gagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCagt                                                                                >  4:147429/1‑147 (MQ=255)
                                                                       ggccggccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCtt                                                                         >  5:77641/1‑149 (MQ=255)
                                                                        gccggccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCttt                                                                        >  3:270830/1‑149 (MQ=255)
                                                                             ccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGTAACCCCGCGAag                                                                                                                                          <  3:45352/78‑1 (MQ=38)
                                                                             ccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGTAACCCCGCGAag                                                                                                                                          >  4:45352/1‑78 (MQ=38)
                                                                              cAAGGCAGTGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCGGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTg                                                                  <  3:12333/149‑1 (MQ=255)
                                                                              cAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTg                                                                  <  2:78911/149‑1 (MQ=255)
                                                                              cAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTAGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTg                                                                  >  1:6206/1‑149 (MQ=255)
                                                                                 ggCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGgcg                                                               >  5:19634/1‑149 (MQ=255)
                                                                                    agagGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGgcgcgc                                                            <  7:103871/149‑1 (MQ=255)
                                                                                         aggTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAg                                                       <  4:270830/149‑1 (MQ=255)
                                                                                           gTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGc                                                     <  5:201349/149‑1 (MQ=255)
                                                                                                gccgccGTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCTGGCTTACCTGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATAGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGTCGCGCTACAGGCATAAg                                                <  8:158891/149‑1 (MQ=255)
                                                                                                gccgccCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCAt                                                                                 <  5:3281/116‑1 (MQ=255)
                                                                                                gccgccCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCAt                                                                                 >  6:3281/1‑116 (MQ=255)
                                                                                                              agtagtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACAATGTCGGCATTTAGCAATTTAACTGGAGCGCGACACGCATTATGTATACGCAGtta                                   >  8:355502/1‑147 (MQ=255)
                                                                                                                tagtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCaaa                                >  1:252542/1‑149 (MQ=255)
                                                                                                                tagtTTATTCCTGTACCGCCTCTTCGCGGGGGTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACGGGCGCGCGACAGGCTTAAGGTTTACGCCGTTCaaa                                >  5:179911/1‑149 (MQ=255)
                                                                                                                 agtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCaaa                                <  3:136754/148‑1 (MQ=255)
                                                                                                                  gtTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAAtt                              >  5:111477/1‑149 (MQ=255)
                                                                                                                       ttCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTg                         <  7:355502/149‑1 (MQ=255)
                                                                                                                        tCCTGTACCGCCAATTAGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTTACGCAAGACACATTCTAGTCATTTAACCTTTTAACTGGCGCGCCACAGGCATAAGGTATACGCCCTTCAAAATACCTg                         >  8:1861/1‑148 (MQ=255)
                                                                                                                          cTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTAGTCATTTAGCCTTTTAACTGGCGCGCGACAGGCATAAGGTATAAGCCGTTCAAATTACCTGGAg                      >  6:287627/1‑149 (MQ=255)
                                                                                                                              aCCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGt                  >  2:212165/1‑149 (MQ=255)
                                                                                                                               ccGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGt                  <  7:1861/148‑1 (MQ=255)
                                                                                                                                 gCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTc                             >  5:338978/1‑135 (MQ=255)
                                                                                                                                 gCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTc                             <  6:338978/135‑1 (MQ=255)
                                                                                                                                  ccTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATg              >  4:247327/1‑149 (MQ=255)
                                                                                                                                   ctctTCGCGGGTAACCCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCCCTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGt             >  3:224567/1‑149 (MQ=255)
                                                                                                                                    tctTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTc            >  4:3375/1‑149 (MQ=255)
                                                                                                                                      ttCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTcc           >  1:182753/1‑148 (MQ=255)
                                                                                                                                        cgcgGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTTCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTcc           <  6:111477/146‑1 (MQ=255)
                                                                                                                                        cgcgGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTccaa         <  2:41317/148‑1 (MQ=255)
                                                                                                                                              gTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTc  <  6:77641/149‑1 (MQ=255)
                                                                                                                                              gTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTc  <  3:3375/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAATTC  >  NC_002947/3312545‑3312835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑