Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,325,702 A→G 12.0% K152R (AAG→AGG)  mqo‑III → malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,325,7020AG12.0% 122.8 / 7.7 51K152R (AAG→AGG) mqo‑IIImalate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (24/20);  new base G (3/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

TTGAAATCAACGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTGGCCGTGACCTGGACGCCG  >  NC_002947/3325558‑3325844
                                                                                                                                                |                                                                                                                                              
ttGAAATCAACGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCTAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGaagaag                                                                                                                                            <  5:524/149‑1 (MQ=255)
   aaaTCAACGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGTAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAgcgc                                                                                                                                         >  2:365123/1‑149 (MQ=255)
     aTCAACGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAgcggat                                                                                                                                       <  5:67728/149‑4 (MQ=255)
     aTCAACGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTa                                                                                                                                       <  1:32055/149‑1 (MQ=255)
        aaCGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCTTCAGGAAgcggatatt                                                                                                                                    <  7:287426/149‑7 (MQ=37)
         aCGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAg                                                                                                                                   <  5:227782/149‑1 (MQ=255)
         aCGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAg                                                                                                                                   >  4:14538/1‑149 (MQ=255)
           gAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAACCCCGCATTCGTTCATCAACCCCACCCCGCACATGAGCTGTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGgc                                                                                                                                 >  4:330732/1‑149 (MQ=255)
             gTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGgcgc                                                                                                                               >  5:204147/1‑149 (MQ=255)
                        tAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGc                                                                                                                    >  1:183580/1‑149 (MQ=255)
                         aaCGCGACATTTTCTATCCTGGCAGGTCAAGCAGGGCGTTCTGAAAAACCAGCATTCGTTCATCAACACAACACCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCAGAAGAAGCGCTACGAGCCGCTGCAGGCCAGcc                                                                                                                   >  6:372539/1‑149 (MQ=255)
                          aCGCGCGAGTTTCTCGCGTGGGAGGTCTAGCAGGGCGTGCTGAAAAACCCGCTTTCGTTCATCAACACGACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCGGCTTCCTGTTGAATCGCTAGGAGGCGCTGGAGGCCAGcc                                                                                                                   <  8:72148/148‑1 (MQ=255)
                            gcgcCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCCCCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGccccc                                                                                                                >  7:311161/1‑149 (MQ=255)
                            gcgcCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGccccc                                                                                                                >  1:166076/1‑149 (MQ=255)
                            gcgcCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGccccc                                                                                                                <  8:69067/149‑1 (MQ=255)
                            gcgcCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGccccc                                                                                                                <  3:254258/149‑1 (MQ=255)
                                    ttCTCGCCTGGCAGCTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCAACGAGCCGCTGCAGGCCAGCCACCTCTTCaaa                                                                                                        >  6:166158/1‑149 (MQ=25)
                                     tctcGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAAc                                                                                                       >  3:244457/1‑149 (MQ=255)
                                        cGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCa                                                                                                    >  3:185932/1‑149 (MQ=255)
                                          ccTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATg                                                                                                  <  5:166158/149‑1 (MQ=255)
                                                aGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCACACCCATGCAGTa                                                                                             >  6:357073/1‑148 (MQ=255)
                                                     aaGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAg                                                                                               >  1:296851/1‑141 (MQ=255)
                                                     aaGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAg                                                                                               <  2:296851/141‑1 (MQ=255)
                                                        cAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGa                                                                                    <  3:14538/149‑1 (MQ=255)
                                                               tGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAg                                                                             <  2:166076/149‑1 (MQ=255)
                                                                 cTGAAAAACGCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTATGGTTCGATGACAATATCCGCTTCCTGAAGAAGCTCTACGAGGCGCTGCAGGTCAGCCCCCTGTTCAAACCAATGCAGTACTCCGAAGACCACTAGc                                                                            <  5:372539/148‑1 (MQ=21)
                                                                  tGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCa                                                                                                <  8:147886/127‑1 (MQ=255)
                                                                  tGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCa                                                                                                >  7:147886/1‑127 (MQ=255)
                                                                  tGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCAc                                                                                <  2:51703/143‑1 (MQ=255)
                                                                  tGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCAc                                                                                >  1:51703/1‑143 (MQ=255)
                                                                          ccGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCa                                                                   >  7:143794/1‑148 (MQ=255)
                                                                                    tcatcaACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGCCCCCGAGCAGATCCGCAAATGGGTCCCg                                                        >  8:173509/1‑149 (MQ=255)
                                                                                     catcaACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCg                                                        <  6:204147/148‑1 (MQ=255)
                                                                                       tcaACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCt                                                      >  8:20523/1‑148 (MQ=255)
                                                                                        caACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAAc                                                                                                       <  1:348063/98‑1 (MQ=255)
                                                                                        caACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAAc                                                                                                       >  2:348063/1‑98 (MQ=255)
                                                                                         aaCACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCtga                                                    <  2:108672/148‑1 (MQ=255)
                                                                                               accCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATgg                                              >  4:194051/1‑148 (MQ=255)
                                                                                                  ccGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAgg                                          <  5:357073/149‑1 (MQ=255)
                                                                                                       caTGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCcgcg                                     <  2:183580/149‑1 (MQ=255)
                                                                                                                tGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCaa                              <  8:143794/147‑1 (MQ=255)
                                                                                                                        gCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTg                    <  1:365123/149‑1 (MQ=255)
                                                                                                                               cAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTGGCCGTGa             >  7:185237/1‑149 (MQ=255)
                                                                                                                                aaTATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTGGCCGTGAc            >  1:52431/1‑149 (MQ=255)
                                                                                                                                   tagcGCTTCTTCAGGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTg                    <  1:37387/135‑1 (MQ=255)
                                                                                                                                   tagcGCTTCTTCAGGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTg                    >  2:37387/4‑138 (MQ=255)
                                                                                                                                    agcGCTTCTTCAGGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCACCCAGAAGCTGGCCGTGACCTg         >  2:243686/3‑148 (MQ=255)
                                                                                                                                     gcGCTTCTTCAGGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAAc                             >  1:246313/2‑127 (MQ=255)
                                                                                                                                     gcGCTTCTTCAGGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAAc                             <  2:246313/126‑1 (MQ=255)
                                                                                                                                     ccGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTGGCCGTGACCTGGa       >  5:89190/1‑149 (MQ=255)
                                                                                                                                        cTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTGGCCGTGACCTGGACGc    >  3:171756/1‑149 (MQ=255)
                                                                                                                                           ccTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTGGCCGTGACCTGGACGCCg  <  4:244457/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TTGAAATCAACGAGTCGTTCCAGGTAACGCGCCAGTTTCTCGCCTGGCAGGTCAAGCAGGGCGTGCTGAAAAACCCGCATTCGTTCATCAACACCACCCCGCACATGAGCTTTGTCTGGGGCGATGACAATATCCGCTTCCTGAAGAAGCGCTACGAGGCGCTGCAGGCCAGCCCCCTGTTCAAACCCATGCAGTACTCCGAAGACCACGAGCAGATCCGCAAATGGGTCCCGCTGATGATGGAAGGCCGCGATCCCAACCAGAAGCTGGCCGTGACCTGGACGCCG  >  NC_002947/3325558‑3325844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑