Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,344,839	T→C	10.4%	D97G (GAC→GGC)	PP_2941 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,344,839	0	T	C	10.4%	174.9 / 9.1	69	D97G (GAC→GGC)	PP_2941	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/32);  new base C (4/3);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CCATTCATACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGG  >  NC_002947/3344691‑3344985                                                                                                                                                     |                                                                                                                                                   ccATTCATACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGt                                                                                                                                                    >  8:71775/1‑149 (MQ=255)    ttCATACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTccg                                                                                                                                                 >  4:298442/1‑149 (MQ=255)            aGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCggg                                                                                                                                         >  3:97770/1‑149 (MQ=255)               cGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCt                                                                                                                                      >  5:76227/1‑149 (MQ=255)                gTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCTGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTg                                                                                                                                     <  4:293197/149‑1 (MQ=255)                   gATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                   <  4:229862/148‑3 (MQ=255)                       ggCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCAt                                                                                                                               <  3:298442/148‑1 (MQ=255)                           ttATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCAcc                                                                                                                          <  3:146541/149‑1 (MQ=255)                                    ggTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGACCGCGGGCCTGCTTCATCCACCCGCAAGAGt                                                                                                                 >  5:205173/1‑149 (MQ=255)                                     gTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTg                                                                                                                <  2:113510/149‑1 (MQ=255)                                         ggCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCAc                                                                                                            >  1:164817/1‑149 (MQ=255)                                         ggCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCAc                                                                                                            <  3:148118/149‑1 (MQ=255)                                         ggCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAAAGCGGTCGCCAGCCCAATCGCACCAGGTCCGCCCGCGGGCCTGCTTCATCCCCCCGCAAGAGTGCCAc                                                                                                            >  3:267339/1‑149 (MQ=255)                                          gCCAGATCGCCCGGAAGAAGGGGCCGTGTTCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                   >  6:270170/1‑123 (MQ=255)                                          gCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                   <  5:270170/125‑3 (MQ=255)                                                tCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCTCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTg                                                                                                     <  2:177413/149‑1 (MQ=255)                                                tCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTg                                                                                                     <  6:113792/149‑1 (MQ=255)                                                   cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCt                                                                                                                                   >  7:116124/1‑116 (MQ=255)                                                   cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCt                                                                                                                                   <  8:116124/116‑1 (MQ=255)                                                   cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTc                                                                                                  <  6:172463/149‑1 (MQ=255)                                                          gaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                           >  7:250559/1‑149 (MQ=255)                                                          gaagGGGCCGGGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                           <  3:253576/149‑1 (MQ=255)                                                              gggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGACCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGtt                                                                                       <  6:205173/149‑1 (MQ=255)                                                              gggCAGTGGGCCACCTTTTCGCCCCTGGCGTTGAGCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGGCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGtt                                                                                       <  3:291672/149‑1 (MQ=255)                                                                        ccACCTTTTCGACCCTGGCGTTGATCGGCGCGGCTCGGTCGGTCGCCCACTGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCGTGCTTCATCCACCCGCAAGAGTTCCACCGGCGTGTTCGGCGCGACGTTGTACCg                                                                                 <  7:180314/145‑1 (MQ=255)                                                                        ccACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGcc                                                                              >  1:49004/1‑148 (MQ=255)                                                                        ccACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCAAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGAGGGTGTACCg                                                                                 >  8:180314/1‑145 (MQ=255)                                                                        ccAACTTTTCGACACTGGCGTTGATCGGCGCGGCACTGTCGGGCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTTTACCGGcc                                                                              <  6:375214/148‑1 (MQ=255)                                                                         cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCa                                                                                                                      >  6:223177/1‑107 (MQ=255)                                                                         cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCa                                                                                                                      <  5:223177/107‑1 (MQ=255)                                                                          aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCg                                                                                 >  4:237029/1‑143 (MQ=255)                                                                          aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCg                                                                                 <  3:237029/143‑1 (MQ=255)                                                                          aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                           >  5:113040/1‑149 (MQ=255)                                                                          aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                           >  8:67129/1‑149 (MQ=255)                                                                          aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                           >  2:99585/1‑149 (MQ=255)                                                                          aCCTTTTCGACCCTGGCGTTGATCGGCGCGCCACGGTCGGTCGCCCAATGCGCTCGCCAGCCCCAACTCACCAGGACCGCCCGCGGGCCTGCTTCATCCACCCGCAAGACTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                           >  7:222/1‑149 (MQ=255)                                                                             ttttCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACAAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                        >  7:222336/1‑149 (MQ=255)                                                                               ttCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                        <  7:103953/147‑1 (MQ=255)                                                                                   aCCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTc                                                                  <  2:204951/149‑1 (MQ=255)                                                                                          cGTTGAGCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACg                                                           <  8:58418/149‑1 (MQ=255)                                                                                              gATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttgt                                                       >  5:185108/1‑149 (MQ=255)                                                                                              gATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttg                                                        <  5:373270/148‑1 (MQ=255)                                                                                                tCGGGGCGGCTCGGTCGGTCGCCGAATGCTGTCGCGAGCCCCATCTCTCCTGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCTAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttgtt                                                      <  8:222/148‑1 (MQ=255)                                                                                                    cgcgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGcc                                                 <  8:250559/149‑1 (MQ=255)                                                                                                    cgcgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                  >  3:263241/1‑148 (MQ=255)                                                                                                     gcgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCa                                                <  1:165634/149‑1 (MQ=255)                                                                                                      cgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAg                                               <  6:76227/149‑1 (MQ=255)                                                                                                      cgGCACGGTCGGTCGCCCAAGGCGGTCGCCAGCCCCAGCTCACCAGGGCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAg                                               <  6:221532/149‑1 (MQ=255)                                                                                                        gCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGc                                             >  4:215026/1‑149 (MQ=255)                                                                                                         cACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGcc                                            <  6:185108/149‑1 (MQ=255)                                                                                                              tcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTgg                                       <  2:164817/149‑1 (MQ=255)                                                                                                                 gtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAg                                    >  1:226137/1‑149 (MQ=255)                                                                                                                   cgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGc                                  >  2:356898/1‑149 (MQ=255)                                                                                                                      ccAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCt                                 >  3:314361/1‑147 (MQ=255)                                                                                                                        aaTGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAgg                             <  3:215026/149‑1 (MQ=255)                                                                                                                             ggTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                        <  7:67129/149‑1 (MQ=255)                                                                                                                               tCGCCAGCCCCATCTCACCAGGCCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGa                      >  2:113770/1‑149 (MQ=255)                                                                                                                                   cAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACaaa                  <  2:226137/149‑1 (MQ=255)                                                                                                                                      ccccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                        >  6:316536/1‑140 (MQ=255)                                                                                                                                      ccccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                        <  5:316536/140‑1 (MQ=255)                                                                                                                                        ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt             <  7:71775/149‑1 (MQ=255)                                                                                                                                        ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt             <  1:356898/149‑1 (MQ=255)                                                                                                                                           atgaagcAGGCCCGCGGGCGGACCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTa          >  7:303751/7‑149 (MQ=255)                                                                                                                                             tcACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATg        >  5:88644/1‑149 (MQ=255)                                                                                                                                               aCCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGcc      >  1:274498/1‑149 (MQ=255)                                                                                                                                               aCCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGATGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGc       >  8:326256/1‑148 (MQ=255)                                                                                                                                                gcAGGCCCGCGGGCGGACCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCt     >  1:179432/2‑149 (MQ=255)                                                                                                                                                 cacgTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGATGTTCTGCCAGGCCTCTGGAAGGCTCAGGGTTTCGGCAAAGTCGTGTATGCCTc    >  8:259286/4‑149 (MQ=255)                                                                                                                                                 cAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTc    >  5:357368/1‑149 (MQ=255)                                                                                                                                                   ggTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCATGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCgg  <  1:350957/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCATTCATACCAGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGG  >  NC_002947/3344691‑3344985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑