Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,344,844 C→G 8.2% R95R (CGG→CGC) ‡ PP_2941 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,344,8440CG 8.2% 203.5 / 4.4 68R95R (CGG→CGC) ‡PP_2941hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/33);  new base G (3/3);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

AGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTACTG  >  NC_002947/3344702‑3344990
                                                                                                                                              |                                                                                                                                                  
aGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCggg                                                                                                                                              >  3:97770/1‑149 (MQ=255)
   cGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCt                                                                                                                                           >  5:76227/1‑149 (MQ=255)
    gTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCTGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTg                                                                                                                                          <  4:293197/149‑1 (MQ=255)
       gATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                        <  4:229862/148‑3 (MQ=255)
           ggCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCAt                                                                                                                                    <  3:298442/148‑1 (MQ=255)
               ttATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCAcc                                                                                                                               <  3:146541/149‑1 (MQ=255)
                        ggTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGACCGCGGGCCTGCTTCATCCACCCGCAAGAGt                                                                                                                      >  5:205173/1‑149 (MQ=255)
                         gTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTg                                                                                                                     <  2:113510/149‑1 (MQ=255)
                             ggCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCAc                                                                                                                 >  1:164817/1‑149 (MQ=255)
                             ggCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCAc                                                                                                                 <  3:148118/149‑1 (MQ=255)
                             ggCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAAAGCGGTCGCCAGCCCAATCGCACCAGGTCCGCCCGCGGGCCTGCTTCATCCCCCCGCAAGAGTGCCAc                                                                                                                 >  3:267339/1‑149 (MQ=255)
                              gCCAGATCGCCCGGAAGAAGGGGCCGTGTTCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                        >  6:270170/1‑123 (MQ=255)
                              gCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGCCCGCGGGCGGACCTggt                                                                                                                                        <  5:270170/125‑3 (MQ=255)
                                    tCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCTCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTg                                                                                                          <  2:177413/149‑1 (MQ=255)
                                    tCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTg                                                                                                          <  6:113792/149‑1 (MQ=255)
                                       cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCt                                                                                                                                        <  8:116124/116‑1 (MQ=255)
                                       cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCt                                                                                                                                        >  7:116124/1‑116 (MQ=255)
                                       cccGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTc                                                                                                       <  6:172463/149‑1 (MQ=255)
                                              gaagGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                                >  7:250559/1‑149 (MQ=255)
                                              gaagGGGCCGGGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGa                                                                                                <  3:253576/149‑1 (MQ=255)
                                                  gggCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGACCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGtt                                                                                            <  6:205173/149‑1 (MQ=255)
                                                  gggCAGTGGGCCACCTTTTCGCCCCTGGCGTTGAGCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGGCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGtt                                                                                            <  3:291672/149‑1 (MQ=255)
                                                            ccACCTTTTCGACCCTGGCGTTGATCGGCGCGGCTCGGTCGGTCGCCCACTGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCGTGCTTCATCCACCCGCAAGAGTTCCACCGGCGTGTTCGGCGCGACGTTGTACCg                                                                                      <  7:180314/145‑1 (MQ=255)
                                                            ccACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGcc                                                                                   >  1:49004/1‑148 (MQ=255)
                                                            ccACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCAAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGAGGGTGTACCg                                                                                      >  8:180314/1‑145 (MQ=255)
                                                            ccAACTTTTCGACACTGGCGTTGATCGGCGCGGCACTGTCGGGCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTTTACCGGcc                                                                                   <  6:375214/148‑1 (MQ=255)
                                                             cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCa                                                                                                                           <  5:223177/107‑1 (MQ=255)
                                                             cACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCa                                                                                                                           >  6:223177/1‑107 (MQ=255)
                                                              aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCg                                                                                      <  3:237029/143‑1 (MQ=255)
                                                              aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCg                                                                                      >  4:237029/1‑143 (MQ=255)
                                                              aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                                >  5:113040/1‑149 (MQ=255)
                                                              aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                                >  8:67129/1‑149 (MQ=255)
                                                              aCCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                                >  2:99585/1‑149 (MQ=255)
                                                              aCCTTTTCGACCCTGGCGTTGATCGGCGCGCCACGGTCGGTCGCCCAATGCGCTCGCCAGCCCCAACTCACCAGGACCGCCCGCGGGCCTGCTTCATCCACCCGCAAGACTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAg                                                                                >  7:222/1‑149 (MQ=255)
                                                                 ttttCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACAAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                             >  7:222336/1‑149 (MQ=255)
                                                                   ttCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTgg                                                                             <  7:103953/147‑1 (MQ=255)
                                                                       aCCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTc                                                                       <  2:204951/149‑1 (MQ=255)
                                                                              cGTTGAGCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACg                                                                <  8:58418/149‑1 (MQ=255)
                                                                                  gATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttgt                                                            >  5:185108/1‑149 (MQ=255)
                                                                                  gATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttg                                                             <  5:373270/148‑1 (MQ=255)
                                                                                    tCGGGGCGGCTCGGTCGGTCGCCGAATGCTGTCGCGAGCCCCATCTCTCCTGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCTAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACgttgtt                                                           <  8:222/148‑1 (MQ=255)
                                                                                        cgcgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGcc                                                      <  8:250559/149‑1 (MQ=255)
                                                                                        cgcgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGc                                                       >  3:263241/1‑148 (MQ=255)
                                                                                         gcgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCa                                                     <  1:165634/149‑1 (MQ=255)
                                                                                          cgGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAg                                                    <  6:76227/149‑1 (MQ=255)
                                                                                          cgGCACGGTCGGTCGCCCAAGGCGGTCGCCAGCCCCAGCTCACCAGGGCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAg                                                    <  6:221532/149‑1 (MQ=255)
                                                                                            gCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGc                                                  >  4:215026/1‑149 (MQ=255)
                                                                                             cACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGcc                                                 <  6:185108/149‑1 (MQ=255)
                                                                                                  tcggtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTgg                                            <  2:164817/149‑1 (MQ=255)
                                                                                                     gtcgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAg                                         >  1:226137/1‑149 (MQ=255)
                                                                                                       cgCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGc                                       >  2:356898/1‑149 (MQ=255)
                                                                                                          ccAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCt                                      >  3:314361/1‑147 (MQ=255)
                                                                                                            aaTGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAgg                                  <  3:215026/149‑1 (MQ=255)
                                                                                                                 ggTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                             <  7:67129/149‑1 (MQ=255)
                                                                                                                   tCGCCAGCCCCATCTCACCAGGCCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGa                           >  2:113770/1‑149 (MQ=255)
                                                                                                                       cAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACaaa                       <  2:226137/149‑1 (MQ=255)
                                                                                                                          ccccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                             <  5:316536/140‑1 (MQ=255)
                                                                                                                          ccccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTc                             >  6:316536/1‑140 (MQ=255)
                                                                                                                            ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt                  <  7:71775/149‑1 (MQ=255)
                                                                                                                            ccATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCgt                  <  1:356898/149‑1 (MQ=255)
                                                                                                                               atgaagcAGGCCCGCGGGCGGACCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTa               >  7:303751/7‑149 (MQ=255)
                                                                                                                                 tcACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATg             >  5:88644/1‑149 (MQ=255)
                                                                                                                                   aCCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGcc           >  1:274498/1‑149 (MQ=255)
                                                                                                                                   aCCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGATGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGc            >  8:326256/1‑148 (MQ=255)
                                                                                                                                    gcAGGCCCGCGGGCGGACCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCt          >  1:179432/2‑149 (MQ=255)
                                                                                                                                     cacgTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGATGTTCTGCCAGGCCTCTGGAAGGCTCAGGGTTTCGGCAAAGTCGTGTATGCCTc         >  8:259286/4‑149 (MQ=255)
                                                                                                                                     cAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTc         >  5:357368/1‑149 (MQ=255)
                                                                                                                                       ggTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCATGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCgg       <  1:350957/149‑1 (MQ=255)
                                                                                                                                            gcccgcGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTCCTg  >  5:268253/1‑149 (MQ=255)
                                                                                                                                            gcccgcGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTACTg  >  3:104817/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
AGCCGTCGATCGGCGTTATGCCTCGGTGCGGCCAGATCGCCCGGAAGAAGGGGCCGTGTGCCACCTTTTCGACCCTGGCGTTGATCGGCGCGGCACGGTCGGTCGCCCAATGCGGTCGCCAGCCCCATCTCACCAGGTCCGCCCGCGGGCCTGCTTCATCCACCCGCAAGAGTGCCACCGGCGTGGTCGGCGCGACGTTGTACCGGCCAAGTGGCTGGTCGCCGACGTTGTTCTGCCAGGCCTCTGGCAGGCTCAGGGTTTCGACAAAGTCGTGTATGCCTCGGTACTG  >  NC_002947/3344702‑3344990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑