Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,453,480 A→G 6.2% intergenic (‑34/+79) PP_3069 ← / ← ppiC‑I outer membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,453,4800AG 6.2% 235.5 / 4.3 80intergenic (‑34/+79)PP_3069/ppiC‑Iouter membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base A (33/42);  new base G (3/2);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

CCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGT  >  NC_002947/3453334‑3453619
                                                                                                                                                  |                                                                                                                                           
cccGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTGGTGcgca                                                                                                                                           <  6:166781/149‑1 (MQ=255)
 ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTGGTGCGCAg                                                                                                                                          <  3:247103/149‑1 (MQ=255)
  cgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCTGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAACATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAgg                                                                                                                                         <  1:258847/149‑1 (MQ=255)
  cggcccgtgTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAgg                                                                                                                                         <  8:140585/149‑1 (MQ=255)
   gggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAggg                                                                                                                                        >  5:311945/1‑149 (MQ=255)
        gggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTAcc                                                                                                                                   >  1:13001/1‑149 (MQ=255)
        gggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTAcc                                                                                                                                   <  4:326876/149‑1 (MQ=255)
         ggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCg                                                                                                                                  <  2:307498/149‑1 (MQ=255)
         ggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCg                                                                                                                                  <  7:15249/149‑1 (MQ=255)
             agaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCCGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGt                                                                                                                              >  7:323739/1‑149 (MQ=255)
              gaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGtt                                                                                                                             <  8:268361/149‑1 (MQ=255)
                aGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTg                                                                                                                            <  8:311546/148‑1 (MQ=255)
                aGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGt                                                                                                                           <  1:274937/149‑1 (MQ=255)
                  gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCCCGCGTGGTGCACAGGGTTACCGGGGTTGtat                                                                                                                         >  2:189883/1‑149 (MQ=255)
                  gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAg                                                                                                                                          <  1:60476/132‑1 (MQ=255)
                  gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAg                                                                                                                                          >  2:60476/1‑132 (MQ=255)
                  gTACAAGCCGCGGGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGtat                                                                                                                         <  7:189479/149‑1 (MQ=255)
                   tACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGtata                                                                                                                        <  7:262982/149‑1 (MQ=255)
                      aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATagag                                                                                                                     <  5:219013/149‑1 (MQ=255)
                      aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATagag                                                                                                                     >  5:10901/1‑149 (MQ=255)
                      aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATagag                                                                                                                     <  2:250902/149‑1 (MQ=255)
                       aGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATagag                                                                                                                     >  4:185021/1‑148 (MQ=255)
                       aGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATagag                                                                                                                     >  7:157129/1‑148 (MQ=255)
                        gCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAgagga                                                                                                                   <  2:334908/149‑1 (MQ=255)
                        gCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAgagga                                                                                                                   <  2:329452/149‑1 (MQ=255)
                          cgcgTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCTGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGt                                                                                                                 <  1:305298/149‑1 (MQ=255)
                              tgtgCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGt                                                                                                             <  6:235816/149‑1 (MQ=255)
                                tgCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGcgca                                                                                                                                           >  4:179864/1‑117 (MQ=255)
                                tgCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGcgca                                                                                                                                           <  3:179864/117‑1 (MQ=255)
                                   aGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGa                                                                                                        <  6:311945/149‑1 (MQ=255)
                                       ggCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACcac                                                                                                    <  4:226682/149‑1 (MQ=255)
                                         cGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGTGGAATGCGTCACGACCACaa                                                                                                  <  2:13001/149‑1 (MQ=255)
                                            ggCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTc                                                                                                            <  5:286552/136‑1 (MQ=255)
                                            ggCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTc                                                                                                            >  6:286552/1‑136 (MQ=255)
                                                   cccAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGGGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCaaaaa                                                                                        <  1:189883/149‑1 (MQ=255)
                                                    ccAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACGGGGGGTGTATAGAGGAGTGCGTCACGAGCACAACTGGcgacact                                                                                       >  6:205739/1‑143 (MQ=255)
                                                    ccAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAAt                                                                                       >  2:42335/1‑149 (MQ=255)
                                                                gCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCa                                                                            >  3:188662/1‑148 (MQ=255)
                                                                gCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCa                                                                            >  3:364582/1‑148 (MQ=255)
                                                                  gAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGc                                                                                                               <  8:165725/111‑1 (MQ=255)
                                                                  gAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGc                                                                                                               >  7:165725/1‑111 (MQ=255)
                                                                   aaCCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAgg                                                                        >  2:78357/1‑149 (MQ=255)
                                                                      ccAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAgg                                                                        >  3:307112/1‑146 (MQ=255)
                                                                      ccAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAgg                                                                        <  4:307112/146‑1 (MQ=255)
                                                                           gTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAgcgc                                                                >  5:228899/1‑149 (MQ=255)
                                                                            tCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAgagga                                                                                                                   >  6:176300/1‑97 (MQ=255)
                                                                            tCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAgagga                                                                                                                   <  5:176300/97‑1 (MQ=255)
                                                                              aGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGt                                                              <  6:228899/148‑1 (MQ=255)
                                                                              aGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACAGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGt                                                              <  8:157129/148‑1 (MQ=255)
                                                                                  cccgatctAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTa                                                            <  3:43204/139‑1 (MQ=255)
                                                                                    aGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTcg                                                        <  8:323739/148‑1 (MQ=255)
                                                                                      gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGtt                                                             >  7:345651/1‑141 (MQ=255)
                                                                                      gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGtt                                                             <  8:345651/141‑1 (MQ=255)
                                                                                        gTAGACGCATCAGGAAAACCTCCAATCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTGAGTCGCgg                                                     >  4:60216/1‑147 (MQ=255)
                                                                                           gACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTa                                                            >  4:43204/1‑137 (MQ=255)
                                                                                            actcATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAAc                                               <  3:204054/146‑1 (MQ=255)
                                                                                                aTCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGttt                                           <  1:244793/149‑1 (MQ=255)
                                                                                                   aGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCACGGTAGAACGTTTGCa                                        >  4:366035/1‑149 (MQ=255)
                                                                                                    ggAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCAc                                       >  5:185152/1‑149 (MQ=255)
                                                                                                     gAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCAc                                       >  5:263749/1‑148 (MQ=255)
                                                                                                      aaaaCCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCa                                     <  1:180968/149‑1 (MQ=255)
                                                                                                       aaaCCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAg                                    <  6:10901/149‑1 (MQ=255)
                                                                                                        aaCCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAgg                                   <  6:185152/149‑1 (MQ=255)
                                                                                                           cTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtgg                                >  1:245838/1‑149 (MQ=255)
                                                                                                             ccATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGGGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTa                              <  7:196968/149‑1 (MQ=255)
                                                                                                               aTTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtgg                                >  1:281873/1‑145 (MQ=255)
                                                                                                               aTTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtgg                                <  2:281873/145‑1 (MQ=255)
                                                                                                                ttCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGTCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACAAGGTGGTAGcg                           >  8:125617/1‑148 (MQ=255)
                                                                                                                     taGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCATAAATGTCAGGTGGCAAAGGGCGAGCGCGGTAGTCGCGGTAGAACGTGTGCACCAGGTGGTAGcgtcatt                      >  4:24856/2‑143 (MQ=255)
                                                                                                                     cAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAAtt                      >  8:323370/1‑149 (MQ=255)
                                                                                                                           aaCCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGctct                <  1:42335/149‑1 (MQ=255)
                                                                                                                             ccTGTGCACCACGCGTGGTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCtt               >  2:26257/1‑148 (MQ=255)
                                                                                                                             ccTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCtt               >  5:23956/1‑148 (MQ=255)
                                                                                                                              cTGTGCACCACGCGTGGTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGa             >  5:347188/1‑149 (MQ=255)
                                                                                                                               tgtgcACCACGCGTGGTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGttt                                           <  4:25934/115‑1 (MQ=255)
                                                                                                                               tgtgcACCACGCGTGGTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGAt            >  4:50254/4‑149 (MQ=255)
                                                                                                                               tGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGctc                 <  3:215375/144‑1 (MQ=255)
                                                                                                                               tGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGctc                 >  4:215375/1‑144 (MQ=255)
                                                                                                                                     ccacGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTCGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGccc      <  1:78357/149‑1 (MQ=255)
                                                                                                                                      cacGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCg     >  3:350402/1‑149 (MQ=255)
                                                                                                                                       acGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCgt    <  6:263749/149‑1 (MQ=255)
                                                                                                                                        cgcgTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCgtg   >  4:109865/1‑149 (MQ=255)
                                                                                                                                         gcgTGGTGCAAAGGGTTACCGTGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGTTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCgtgt  <  3:79756/149‑1 (MQ=255)
                                                                                                                                                  acaGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAg                                                                   <  2:42847/75‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
CCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGT  >  NC_002947/3453334‑3453619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑