Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,462,958 A→G 6.0% F467S (TTC→TCC)  PP_3078 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,462,9580AG 6.0% 195.8 / 2.4 67F467S (TTC→TCC) PP_3078ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (34/29);  new base G (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

TCATGTCGTAGTCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACAC  >  NC_002947/3462812‑3463106
                                                                                                                                                    |                                                                                                                                                    
tCATGTCGTAGTCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAAc                                                                                                                                                    >  5:69111/1‑149 (MQ=255)
 cATGTCGTAGTCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCAGTGAGGAACGTGAACg                                                                                                                                                   >  5:112535/1‑149 (MQ=255)
  aTGTCGTAGTCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCCGCCTGGCCATTGAGGAACGTGAAc                                                                                                                                                    <  1:5926/148‑1 (MQ=255)
    gTCGTAGTCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATATGAAAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGc                                                                                                                                                  >  1:297303/1‑148 (MQ=255)
     tCGTAGTCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCTGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAg                                                                                                                                               <  3:225781/149‑1 (MQ=255)
           tCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGt                                                                                                                                         <  1:236470/149‑1 (MQ=255)
                cgcCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCt                                                                                                                                    >  7:47051/1‑149 (MQ=255)
                  ccATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTgg                                                                                                                                  <  7:340046/149‑1 (MQ=255)
                   cATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑ACCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGc                                                                                                                                 >  5:308669/1‑149 (MQ=255)
                            gTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCa                                                                                                                        <  1:256040/149‑1 (MQ=255)
                              ttaCGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAgg                                                                                                                      <  3:297937/147‑1 (MQ=255)
                                aCGTACTGGACCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCg                                                                                                                    >  1:137531/1‑149 (MQ=255)
                                   tACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTc                                                                                                                 <  8:233725/149‑1 (MQ=255)
                                        ggCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCtt                                                                                                            >  5:345910/1‑149 (MQ=255)
                                          ccGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCg                                                                                                          <  6:345910/149‑1 (MQ=255)
                                           cGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCgg                                                                                                         <  5:163153/149‑1 (MQ=255)
                                              gTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGt                                                                                                      <  2:137531/149‑1 (MQ=255)
                                                 gACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGcc                                                                                                   >  8:303934/1‑149 (MQ=255)
                                                 gACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGcc                                                                                                   >  6:160967/1‑149 (MQ=255)
                                                 gACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGc                                                                                                    >  4:104518/1‑148 (MQ=255)
                                                    gTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGTCGATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTTTTGACCAGGCGGTCGCCTTCGGGGTGCCAGc                                                                                                >  5:48759/1‑149 (MQ=255)
                                                         ggATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTAt                                                                                                                              <  4:289756/114‑1 (MQ=255)
                                                             at‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGtt                                                                                       >  1:92223/1‑149 (MQ=255)
                                                             at‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGtt                                                                                       >  5:249187/1‑149 (MQ=255)
                                                              t‑tCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTAGGGGTGCCAGCAGGCTTGTTc                                                                                      >  1:248983/1‑149 (MQ=255)
                                                                  aGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGCCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAg                                                                                   <  7:294795/149‑1 (MQ=255)
                                                                    ggTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAg                                                                                   >  1:87378/1‑147 (MQ=255)
                                                                        aCGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCCTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGCTGCCAGCCGGCTTGTTCGAGcaggg                                                                              >  2:292944/1‑148 (MQ=255)
                                                                         cGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGcagggcc                                                                            <  3:104518/149‑1 (MQ=255)
                                                                          gCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGcagggc                                                                             >  7:222895/1‑147 (MQ=255)
                                                                                   ggCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCt                                                                     <  6:327396/146‑1 (MQ=255)
                                                                                    gCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGCCCTGGCCCTGctc                                                                 <  2:297303/149‑3 (MQ=255)
                                                                                     ccAGGTTGCGCTTCCTCGGCAGCAGGAGGCGTTCAAGG‑CCCGGCTGGCCATTGAGGAACGTGAACGCCAGCGGCGGGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAAc                                                                <  7:345387/149‑1 (MQ=255)
                                                                                     ccAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAAc                                                                >  4:216536/1‑149 (MQ=255)
                                                                                     ccAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGC‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTAGTGACCAGGCGGGCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAAc                                                                >  7:198115/1‑149 (MQ=255)
                                                                                         gTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGcagg                                                                               <  4:13363/130‑1 (MQ=255)
                                                                                         gTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGcagg                                                                               >  3:13363/1‑130 (MQ=255)
                                                                                         gTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAActgct                                                            >  6:78386/1‑149 (MQ=255)
                                                                                               cTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGAc                                                      <  5:246413/149‑1 (MQ=255)
                                                                                                  ccACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACgg                                                    <  2:87378/148‑1 (MQ=255)
                                                                                                  ccACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGga                                                   >  3:321589/1‑149 (MQ=255)
                                                                                                  ccACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGga                                                   >  3:344367/1‑149 (MQ=255)
                                                                                                   cACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTg                                                                    <  5:1185/131‑1 (MQ=255)
                                                                                                   cACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTg                                                                    >  6:1185/1‑131 (MQ=255)
                                                                                                   cACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGga                                                   <  5:78386/148‑1 (MQ=255)
                                                                                                   cACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGga                                                   <  6:308669/148‑1 (MQ=255)
                                                                                                    aCGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGgat                                                  >  7:306480/1‑148 (MQ=255)
                                                                                                              cagGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGGACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGgat                                                  >  6:103378/1‑138 (MQ=255)
                                                                                                              cagGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGGACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGgat                                                  <  5:103378/138‑1 (MQ=255)
                                                                                                                      cAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGt                                <  6:69111/148‑1 (MQ=255)
                                                                                                                         gg‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGAcc                            >  6:99960/1‑149 (MQ=255)
                                                                                                                          g‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCg                           >  8:240645/1‑149 (MQ=255)
                                                                                                                             ccGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCggg                         >  2:139013/1‑149 (MQ=255)
                                                                                                                             ccGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCggg                         <  6:112535/149‑1 (MQ=255)
                                                                                                                                    gCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTg                   <  7:240645/148‑1 (MQ=255)
                                                                                                                                    gCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTg                   <  3:189797/148‑1 (MQ=255)
                                                                                                                                      cATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGaa                 >  5:162915/1‑148 (MQ=255)
                                                                                                                                        ttGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAacac              >  3:221974/1‑149 (MQ=255)
                                                                                                                                          gAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGAGACCGGGGCAGTGAACAcc             <  3:216536/148‑1 (MQ=255)
                                                                                                                                            ggAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCgtg          >  2:172895/1‑149 (MQ=255)
                                                                                                                                             gAACGTGGACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGa                                         <  6:236754/117‑1 (MQ=255)
                                                                                                                                             gAACGTGGACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGa                                         >  5:236754/1‑117 (MQ=255)
                                                                                                                                             gAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTTCCCGGGGCAGTGCACCCCgtgg         >  6:260986/1‑149 (MQ=255)
                                                                                                                                             gAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCgtgg         >  4:16889/1‑149 (MQ=255)
                                                                                                                                             gAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCgtgg         <  2:92223/149‑1 (MQ=255)
                                                                                                                                              aaCGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCgtggt        <  6:249187/149‑1 (MQ=255)
                                                                                                                                               aCGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCgtggt        >  3:337543/1‑148 (MQ=255)
                                                                                                                                                    aaCGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAacac  <  5:260986/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCATGTCGTAGTCGCGCGCCATCAACCGGTTGACGTACTGGGCCGAGTCGACGTTGCGGATAT‑TCAGGGTCACGCCGATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGG‑CCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACAC  >  NC_002947/3462812‑3463106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑