Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,566,174 T→G 8.8% D64A (GAC→GCC)  PP_3148 ← glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,566,1740TG 8.8% 202.0 / 10.6 68D64A (GAC→GCC) PP_3148glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (35/27);  new base G (2/4);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

GATAGGGTTGCCTTGCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGT  >  NC_002947/3566026‑3566321
                                                                                                                                                    |                                                                                                                                                   
gATAGGGTTGCCTAGCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGCTCGCCCACGGGATCTGGTAAACGGCGCTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGACCAGGTTGTAATAGATGTCAtcgt                                                                                                                                                     >  2:195095/1‑149 (MQ=255)
  tAGGGTTGCCTTGCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTTGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCAtcgtcg                                                                                                                                                   <  3:162807/149‑1 (MQ=255)
   aGGGTTGCCTTGCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCAtcgtcg                                                                                                                                                   <  8:122028/148‑1 (MQ=255)
        tGCCTTGCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAg                                                                                                                                             >  3:282143/1‑149 (MQ=255)
              gcttgTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGc                                                                                                                                         <  8:53618/147‑1 (MQ=255)
              gcttgTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCg                                                                                                                                       >  6:202765/1‑149 (MQ=255)
                   tCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGa                                                                                                                                   <  2:52818/148‑1 (MQ=255)
                   tCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGAc                                                                                                                                  >  6:119968/1‑149 (MQ=255)
                     gAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAca                                                                                                                                 <  1:150499/148‑2 (MQ=255)
                         gtgtCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatctat                                                                                                                            <  1:176481/149‑7 (MQ=255)
                          tgtCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgc                                                                                                                           <  5:152925/149‑1 (MQ=255)
                            tCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgca                                                                                                                          <  5:98406/148‑1 (MQ=255)
                               tGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatct                                                                                                                              >  1:54/1‑137 (MQ=255)
                               tGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatct                                                                                                                              <  2:54/141‑5 (MQ=255)
                                   tCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACg                                                                                                                  >  2:261282/1‑149 (MQ=255)
                                     aCGATCTCTGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGTGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCt                                                                                                                <  6:170570/149‑1 (MQ=255)
                                       gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACt                                                                                                                                 >  2:169701/1‑130 (MQ=255)
                                       gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACt                                                                                                                                 <  1:169701/130‑1 (MQ=255)
                                       gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCtt                                                                                                               >  3:31477/1‑148 (MQ=255)
                                         tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTTGGATCGGGGCGGAAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                            >  8:189949/1‑149 (MQ=255)
                                         tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                            >  5:149465/1‑149 (MQ=255)
                                         tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                            <  5:202765/149‑1 (MQ=255)
                                         tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                            <  4:282143/149‑1 (MQ=255)
                                          cgcgGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                            <  8:232711/148‑1 (MQ=255)
                                           gcgGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                          <  7:144272/149‑1 (MQ=255)
                                                   cTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGCCTGTCTGCAGCAGCACGCTTTCTGGCAGACGCa                                                                                                  >  6:316745/1‑149 (MQ=255)
                                                            ccACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGc                                                                                          >  3:20368/1‑148 (MQ=255)
                                                            ccACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCt                                                                                         >  5:129870/1‑149 (MQ=255)
                                                             cACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                          >  3:232549/1‑131 (MQ=255)
                                                             cACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                          <  4:232549/131‑1 (MQ=255)
                                                              aCGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCa                                                                                       >  1:156851/1‑149 (MQ=255)
                                                                gggATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATg                                                                                     <  1:195095/149‑1 (MQ=255)
                                                                   atttGGTAAACGGCGGTGGTATAGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTTTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggag                                                                                  <  7:290257/146‑1 (MQ=255)
                                                                   aTCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggag                                                                                  >  1:19209/1‑149 (MQ=255)
                                                                      tGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggagg                                                                                 <  7:329942/147‑1 (MQ=255)
                                                                      tGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggagg                                                                                 >  8:329942/1‑147 (MQ=255)
                                                                           aaCGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcat                                                                                                                                <  3:220033/95‑3 (MQ=37)
                                                                           aaCGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcat                                                                                                                                >  4:220033/1‑93 (MQ=37)
                                                                                cggTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgcag                                                                                                                         <  2:75491/97‑1 (MQ=255)
                                                                                   tGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGgc                                                                  >  5:50109/1‑149 (MQ=255)
                                                                                       aTCGGGGCGGCAGACCATGGCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCaa                                                              <  4:31477/149‑1 (MQ=255)
                                                                                        tCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTg                                                                           <  5:321742/135‑1 (MQ=255)
                                                                                        tCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTg                                                                           >  6:321742/1‑135 (MQ=255)
                                                                                        tCGGGGCGGCAGACCATCTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGAAGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGAAGCATGCCTCGCTCATGGAGGAACTTGGAGGTAGGCGCaa                                                              >  2:15058/1‑148 (MQ=255)
                                                                                               ggcAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCcg                                                      <  2:19209/149‑1 (MQ=255)
                                                                                                gcAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCcgc                                                     >  1:151058/1‑149 (MQ=255)
                                                                                                      cATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGa                                               >  4:220374/1‑149 (MQ=255)
                                                                                                        tgtcgatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATg                                             >  6:274225/1‑149 (MQ=255)
                                                                                                           cgctgtcgGCGGCGTCGAGCAGGTGGTAATAGATGTCATCGTCGACGTAGTCGCCGGGGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCg                                          <  8:338277/146‑1 (MQ=255)
                                                                                                            gatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCgg                                         <  2:156851/149‑1 (MQ=255)
                                                                                                            gatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCgg                                         >  3:18299/1‑149 (MQ=255)
                                                                                                               gtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCgg                                                   <  8:300962/136‑1 (MQ=255)
                                                                                                               gtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCgg                                                   >  7:300962/1‑136 (MQ=255)
                                                                                                                  ggcggcGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGt                                   <  7:340770/149‑1 (MQ=255)
                                                                                                                      gcgTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGACGATGCCGGTCAAGTCGCt                               <  7:189949/149‑1 (MQ=255)
                                                                                                                        gTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGa                             >  3:248931/1‑149 (MQ=255)
                                                                                                                         tCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGAt                            <  4:20368/149‑1 (MQ=255)
                                                                                                                              cAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACg                        >  1:106509/1‑148 (MQ=255)
                                                                                                                                 gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                    >  7:287267/1‑149 (MQ=255)
                                                                                                                                 gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                    >  8:11443/1‑149 (MQ=255)
                                                                                                                                 gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTAATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                    >  3:66104/1‑149 (MQ=255)
                                                                                                                                  ttGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTc                   <  7:11443/149‑1 (MQ=255)
                                                                                                                                  ttGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTc                   >  4:307458/1‑149 (MQ=255)
                                                                                                                                     tAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGAc                >  2:272952/1‑149 (MQ=255)
                                                                                                                                         aGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg            >  7:66181/1‑149 (MQ=255)
                                                                                                                                          gATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg            <  7:339851/148‑1 (MQ=255)
                                                                                                                                          gATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg            <  5:274225/148‑1 (MQ=255)
                                                                                                                                           aTGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGt          >  3:284482/1‑149 (MQ=255)
                                                                                                                                                aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATcc     >  6:266615/1‑149 (MQ=255)
                                                                                                                                                aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATcc     >  6:116754/1‑149 (MQ=255)
                                                                                                                                                aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGAAGAAGCATGACTCGCTCATTGAGGAACTTGTCGGTAGGAGCAATCTTGACGCAGGCGATGCCGGTCAAGTCGCTGATCCGGCAGTCGACTTCGGTGATcc     >  4:286619/1‑149 (MQ=255)
                                                                                                                                                   gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt  <  8:66181/149‑1 (MQ=255)
                                                                                                                                                   gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt  >  8:253374/1‑149 (MQ=255)
                                                                                                                                                   gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt  <  5:116754/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATAGGGTTGCCTTGCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGT  >  NC_002947/3566026‑3566321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑