Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,566,186 C→A 8.3% G60V (GGC→GTC)  PP_3148 ← glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,566,1860CA 8.3% 212.9 / 9.2 72G60V (GGC→GTC) PP_3148glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (37/29);  new base A (2/4);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01

GCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCTTTC  >  NC_002947/3566040‑3566329
                                                                                                                                                  |                                                                                                                                               
gcttgTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGc                                                                                                                                                 <  8:53618/147‑1 (MQ=255)
gcttgTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCg                                                                                                                                               >  6:202765/1‑149 (MQ=255)
     tCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGa                                                                                                                                           <  2:52818/148‑1 (MQ=255)
     tCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGAc                                                                                                                                          >  6:119968/1‑149 (MQ=255)
       gAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAca                                                                                                                                         <  1:150499/148‑2 (MQ=255)
           gtgtCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatctat                                                                                                                                    <  1:176481/149‑7 (MQ=255)
            tgtCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgc                                                                                                                                   <  5:152925/149‑1 (MQ=255)
              tCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgca                                                                                                                                  <  5:98406/148‑1 (MQ=255)
                 tGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatct                                                                                                                                      >  1:54/1‑137 (MQ=255)
                 tGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatct                                                                                                                                      <  2:54/141‑5 (MQ=255)
                     tCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACg                                                                                                                          >  2:261282/1‑149 (MQ=255)
                       aCGATCTCTGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGTGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCt                                                                                                                        <  6:170570/149‑1 (MQ=255)
                         gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACt                                                                                                                                         >  2:169701/1‑130 (MQ=255)
                         gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACt                                                                                                                                         <  1:169701/130‑1 (MQ=255)
                         gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCtt                                                                                                                       >  3:31477/1‑148 (MQ=255)
                           tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTTGGATCGGGGCGGAAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    >  8:189949/1‑149 (MQ=255)
                           tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    >  5:149465/1‑149 (MQ=255)
                           tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    <  5:202765/149‑1 (MQ=255)
                           tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    <  4:282143/149‑1 (MQ=255)
                            cgcgGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    <  8:232711/148‑1 (MQ=255)
                             gcgGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                                  <  7:144272/149‑1 (MQ=255)
                                     cTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGCCTGTCTGCAGCAGCACGCTTTCTGGCAGACGCa                                                                                                          >  6:316745/1‑149 (MQ=255)
                                              ccACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGc                                                                                                  >  3:20368/1‑148 (MQ=255)
                                              ccACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCt                                                                                                 >  5:129870/1‑149 (MQ=255)
                                               cACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                                  >  3:232549/1‑131 (MQ=255)
                                               cACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                                  <  4:232549/131‑1 (MQ=255)
                                                aCGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCa                                                                                               >  1:156851/1‑149 (MQ=255)
                                                  gggATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATg                                                                                             <  1:195095/149‑1 (MQ=255)
                                                     atttGGTAAACGGCGGTGGTATAGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTTTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggag                                                                                          <  7:290257/146‑1 (MQ=255)
                                                     aTCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggag                                                                                          >  1:19209/1‑149 (MQ=255)
                                                        tGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggagg                                                                                         >  8:329942/1‑147 (MQ=255)
                                                        tGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggagg                                                                                         <  7:329942/147‑1 (MQ=255)
                                                             aaCGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcat                                                                                                                                        <  3:220033/95‑3 (MQ=37)
                                                             aaCGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcat                                                                                                                                        >  4:220033/1‑93 (MQ=37)
                                                                  cggTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgcag                                                                                                                                 <  2:75491/97‑1 (MQ=255)
                                                                     tGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGgc                                                                          >  5:50109/1‑149 (MQ=255)
                                                                         aTCGGGGCGGCAGACCATGGCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCaa                                                                      <  4:31477/149‑1 (MQ=255)
                                                                          tCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTg                                                                                   <  5:321742/135‑1 (MQ=255)
                                                                          tCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTg                                                                                   >  6:321742/1‑135 (MQ=255)
                                                                          tCGGGGCGGCAGACCATCTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGAAGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGAAGCATGCCTCGCTCATGGAGGAACTTGGAGGTAGGCGCaa                                                                      >  2:15058/1‑148 (MQ=255)
                                                                                 ggcAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCcg                                                              <  2:19209/149‑1 (MQ=255)
                                                                                  gcAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCcgc                                                             >  1:151058/1‑149 (MQ=255)
                                                                                        cATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGa                                                       >  4:220374/1‑149 (MQ=255)
                                                                                          tgtcgatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATg                                                     >  6:274225/1‑149 (MQ=255)
                                                                                             cgctgtcgGCGGCGTCGAGCAGGTGGTAATAGATGTCATCGTCGACGTAGTCGCCGGGGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCg                                                  <  8:338277/146‑1 (MQ=255)
                                                                                              gatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCgg                                                 <  2:156851/149‑1 (MQ=255)
                                                                                              gatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCgg                                                 >  3:18299/1‑149 (MQ=255)
                                                                                                 gtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCgg                                                           <  8:300962/136‑1 (MQ=255)
                                                                                                 gtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCgg                                                           >  7:300962/1‑136 (MQ=255)
                                                                                                    ggcggcGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGt                                           <  7:340770/149‑1 (MQ=255)
                                                                                                        gcgTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGACGATGCCGGTCAAGTCGCt                                       <  7:189949/149‑1 (MQ=255)
                                                                                                          gTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGa                                     >  3:248931/1‑149 (MQ=255)
                                                                                                           tCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGAt                                    <  4:20368/149‑1 (MQ=255)
                                                                                                                cAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACg                                >  1:106509/1‑148 (MQ=255)
                                                                                                                   gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                            >  7:287267/1‑149 (MQ=255)
                                                                                                                   gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                            >  8:11443/1‑149 (MQ=255)
                                                                                                                   gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTAATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                            >  3:66104/1‑149 (MQ=255)
                                                                                                                    ttGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTc                           <  7:11443/149‑1 (MQ=255)
                                                                                                                    ttGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTc                           >  4:307458/1‑149 (MQ=255)
                                                                                                                       tAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGAc                        >  2:272952/1‑149 (MQ=255)
                                                                                                                           aGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg                    >  7:66181/1‑149 (MQ=255)
                                                                                                                            gATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg                    <  5:274225/148‑1 (MQ=255)
                                                                                                                            gATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg                    <  7:339851/148‑1 (MQ=255)
                                                                                                                             aTGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGt                  >  3:284482/1‑149 (MQ=255)
                                                                                                                                  aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATcc             >  6:116754/1‑149 (MQ=255)
                                                                                                                                  aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATcc             >  6:266615/1‑149 (MQ=255)
                                                                                                                                  aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGAAGAAGCATGACTCGCTCATTGAGGAACTTGTCGGTAGGAGCAATCTTGACGCAGGCGATGCCGGTCAAGTCGCTGATCCGGCAGTCGACTTCGGTGATcc             >  4:286619/1‑149 (MQ=255)
                                                                                                                                     gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt          <  8:66181/149‑1 (MQ=255)
                                                                                                                                     gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt          >  8:253374/1‑149 (MQ=255)
                                                                                                                                     gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt          <  5:116754/149‑1 (MQ=255)
                                                                                                                                         aCGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTc      >  4:13358/1‑149 (MQ=255)
                                                                                                                                           gTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCtt    >  4:44569/1‑149 (MQ=255)
                                                                                                                                              gTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCTTTc  <  7:253374/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCTTGTCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCTTTC  >  NC_002947/3566040‑3566329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑