Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	333,174	C→T	7.4%	H105Y (CAC→TAC)	PP_5435 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	333,174	0	C	T	7.4%	193.1 / 4.4	67	H105Y (CAC→TAC)	PP_5435	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/32);  new base T (3/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

GATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCT  >  NC_002947/333027‑333321                                                                                                                                                    |                                                                                                                                                     gATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCa                                                                                                                                                     >  3:335619/1‑149 (MQ=255) gATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCa                                                                                                                                                     >  4:350051/1‑149 (MQ=255)        tCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAg                                                                                                                                              >  8:300656/1‑149 (MQ=255)          ccccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGt                                                                                                                                             <  1:43111/148‑1 (MQ=255)           cccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                           >  3:226647/1‑149 (MQ=255)           cccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                           >  5:189215/1‑149 (MQ=255)            ccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTCCGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGTCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACg                                                                                                                                          <  5:124163/149‑1 (MQ=255)            ccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                           >  2:256277/1‑148 (MQ=255)             cACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGa                                                                                                                                         <  7:300656/149‑1 (MQ=255)                tCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑a                                                                                                                                      >  7:152712/1‑148 (MQ=255)                tCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑a                                                                                                                                      >  6:329347/1‑148 (MQ=255)                  aaaaCTCAGTCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGGCTGGCGCAAAGCGCGACCGGGGACTCTCTACAGCAACGCCATGTTGTCCCACGCCGAGTACGCC‑AAAt                                                                                                                                   >  4:233072/1‑149 (MQ=255)                  aaaaCTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAAt                                                                                                                                   <  7:301676/149‑1 (MQ=255)                  aaaaCTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAAt                                                                                                                                   >  8:284278/1‑149 (MQ=255)                      cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                   >  6:286292/1‑144 (MQ=255)                      cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                   <  5:281200/145‑2 (MQ=255)                      cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                   <  5:286292/145‑2 (MQ=255)                      cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                   >  6:281200/1‑144 (MQ=255)                         aGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTg                                                                                                                               >  6:309161/1‑146 (MQ=255)                          gccgctccAGCCGCAAACGGCCGCTTGATATCTCCGTGTTCGATATCAAGAAAAACGTATGCCGCCTGATCGCGGGGCTCATTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGa                                                                                                                           <  8:278632/143‑1 (MQ=255)                           gCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAg                                                                                                                            >  1:347571/1‑147 (MQ=255)                             ccGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGc                                                                                                                        >  1:150630/1‑149 (MQ=255)                             ccGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGc                                                                                                                        >  1:3703/1‑149 (MQ=255)                                ccAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGa                                                                                                                      >  5:316189/1‑148 (MQ=255)                                    ccGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCa                                                                                                                 <  8:168240/149‑1 (MQ=255)                                      gCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                           <  1:326184/122‑1 (MQ=255)                                      gCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                           >  2:326184/1‑122 (MQ=255)                                        aaaGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                             <  8:164736/149‑1 (MQ=255)                                                  ttGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTggg                                                                                                   >  7:136552/1‑149 (MQ=255)                                                    gATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAAAGCGCGAACGGGTACTCTCTACATAAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTTCAGAAGCGATATCAGGTAGCAACCTTGGcc                                                                                                 >  1:104594/1‑149 (MQ=255)                                                      tatCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCcgc                                                                                               <  6:189215/149‑1 (MQ=255)                                                            cGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCTAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCcgcgc                                                                                             <  7:284278/145‑1 (MQ=255)                                                            cGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGata                                                                                         <  8:147701/149‑1 (MQ=255)                                                                ttCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                             <  1:26654/125‑1 (MQ=255)                                                                ttCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                             >  2:26654/1‑125 (MQ=255)                                                                   gATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCaa                                                                                   <  1:256277/148‑1 (MQ=255)                                                                   gATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCaa                                                                                   <  4:226647/148‑1 (MQ=255)                                                                         aaGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGTTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACa                                                                            <  2:7317/149‑1 (MQ=255)                                                                         aaGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACa                                                                            <  2:3703/149‑1 (MQ=255)                                                                          aGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAg                                                                           <  5:309161/149‑1 (MQ=255)                                                                                aCGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGCGTACTCTCTACATCAAGGCCATTTTGTCGCACGCGGAGTACGAC‑AAATCGTGCAGAAGCGATATCATGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGac                                                                      >  1:155453/1‑148 (MQ=255)                                                                                 cGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGacaa                                                                    <  8:136552/149‑1 (MQ=255)                                                                                         gccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGc                                                            <  4:369305/149‑1 (MQ=255)                                                                                         gccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGc                                                            <  3:128897/149‑1 (MQ=255)                                                                                          ccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCg                                                           <  2:347571/149‑1 (MQ=255)                                                                                           cTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCgg                                                          <  8:152712/149‑1 (MQ=255)                                                                                              aTCGCGTGGCTGAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCggg                                                         <  3:350051/147‑1 (MQ=255)                                                                                                cgcgTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATa                                                      >  6:167832/1‑148 (MQ=255)                                                                                                    tGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATc                                                 <  6:316189/149‑1 (MQ=255)                                                                                                       cTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                              <  8:344236/149‑1 (MQ=255)                                                                                                       cTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                              <  4:335619/149‑1 (MQ=255)                                                                                                          aaTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAAt                                                                                  <  8:351784/110‑1 (MQ=255)                                                                                                          aaTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGc                                            <  5:329347/148‑1 (MQ=255)                                                                                                                  aaCGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                              >  7:239511/1‑138 (MQ=255)                                                                                                                  aaCGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                              <  8:239511/138‑1 (MQ=255)                                                                                                                       gTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATg                              >  2:80783/1‑149 (MQ=255)                                                                                                                          ctctctACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCt                           >  3:313143/1‑149 (MQ=255)                                                                                                                             tctACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCa                        >  7:327407/1‑149 (MQ=255)                                                                                                                               tACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGat                                                                                                                     >  1:299040/1‑54 (MQ=255)                                                                                                                               tACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGat                                                                                                                     <  2:299040/54‑1 (MQ=255)                                                                                                                                 cATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCAt                       <  4:313143/146‑1 (MQ=255)                                                                                                                                    cAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                 >  5:371428/1‑149 (MQ=255)                                                                                                                                         accatttGTCGTACTCGGCGTGCGACAAAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCAACGCTAATAtt             >  2:15467/5‑149 (MQ=255)                                                                                                                                          cATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATAttt            >  4:175260/1‑148 (MQ=255)                                                                                                                                           aTTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                 <  5:345549/142‑1 (MQ=255)                                                                                                                                           aTTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                 >  6:345549/1‑142 (MQ=255)                                                                                                                                                   gCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCt  >  5:120510/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCT  >  NC_002947/333027‑333321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑