Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,566,189 G→A 8.4% T59I (ACC→ATC)  PP_3148 ← glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,566,1890GA 8.4% 200.3 / 6.2 71T59I (ACC→ATC) PP_3148glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (36/29);  new base A (2/4);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.72e-01

TCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCTTTC  >  NC_002947/3566045‑3566329
                                                                                                                                                |                                                                                                                                            
tCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGa                                                                                                                                           <  2:52818/148‑1 (MQ=255)
tCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGAc                                                                                                                                          >  6:119968/1‑149 (MQ=255)
  gAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAca                                                                                                                                         <  1:150499/148‑2 (MQ=255)
      gtgtCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatctat                                                                                                                                    <  1:176481/149‑7 (MQ=255)
       tgtCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgc                                                                                                                                   <  5:152925/149‑1 (MQ=255)
         tCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgca                                                                                                                                  <  5:98406/148‑1 (MQ=255)
            tGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatct                                                                                                                                      <  2:54/141‑5 (MQ=255)
            tGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcatct                                                                                                                                      >  1:54/1‑137 (MQ=255)
                tCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACg                                                                                                                          >  2:261282/1‑149 (MQ=255)
                  aCGATCTCTGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGTGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCt                                                                                                                        <  6:170570/149‑1 (MQ=255)
                    gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACt                                                                                                                                         <  1:169701/130‑1 (MQ=255)
                    gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACt                                                                                                                                         >  2:169701/1‑130 (MQ=255)
                    gATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCtt                                                                                                                       >  3:31477/1‑148 (MQ=255)
                      tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTTGGATCGGGGCGGAAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    >  8:189949/1‑149 (MQ=255)
                      tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    >  5:149465/1‑149 (MQ=255)
                      tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    <  4:282143/149‑1 (MQ=255)
                      tCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    <  5:202765/149‑1 (MQ=255)
                       cgcgGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCt                                                                                                                    <  8:232711/148‑1 (MQ=255)
                        gcgGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                                  <  7:144272/149‑1 (MQ=255)
                                cTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGCCTGTCTGCAGCAGCACGCTTTCTGGCAGACGCa                                                                                                          >  6:316745/1‑149 (MQ=255)
                                         ccACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGc                                                                                                  >  3:20368/1‑148 (MQ=255)
                                         ccACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCt                                                                                                 >  5:129870/1‑149 (MQ=255)
                                          cACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                                  >  3:232549/1‑131 (MQ=255)
                                          cACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTgg                                                                                                                  <  4:232549/131‑1 (MQ=255)
                                           aCGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCa                                                                                               >  1:156851/1‑149 (MQ=255)
                                             gggATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATg                                                                                             <  1:195095/149‑1 (MQ=255)
                                                atttGGTAAACGGCGGTGGTATAGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTTTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggag                                                                                          <  7:290257/146‑1 (MQ=255)
                                                aTCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggag                                                                                          >  1:19209/1‑149 (MQ=255)
                                                   tGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggagg                                                                                         <  7:329942/147‑1 (MQ=255)
                                                   tGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATggagg                                                                                         >  8:329942/1‑147 (MQ=255)
                                                        aaCGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcat                                                                                                                                        <  3:220033/95‑3 (MQ=37)
                                                        aaCGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCACCGGCGACTACGTCGACGATGAcat                                                                                                                                        >  4:220033/1‑93 (MQ=37)
                                                             cggTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTgcag                                                                                                                                 <  2:75491/97‑1 (MQ=255)
                                                                tGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGgc                                                                          >  5:50109/1‑149 (MQ=255)
                                                                    aTCGGGGCGGCAGACCATGGCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCaa                                                                      <  4:31477/149‑1 (MQ=255)
                                                                     tCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTg                                                                                   >  6:321742/1‑135 (MQ=255)
                                                                     tCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTg                                                                                   <  5:321742/135‑1 (MQ=255)
                                                                     tCGGGGCGGCAGACCATCTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGAAGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGAAGCATGCCTCGCTCATGGAGGAACTTGGAGGTAGGCGCaa                                                                      >  2:15058/1‑148 (MQ=255)
                                                                            ggcAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCcg                                                              <  2:19209/149‑1 (MQ=255)
                                                                             gcAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCcgc                                                             >  1:151058/1‑149 (MQ=255)
                                                                                   cATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGa                                                       >  4:220374/1‑149 (MQ=255)
                                                                                     tgtcgatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATg                                                     >  6:274225/1‑149 (MQ=255)
                                                                                        cgctgtcgGCGGCGTCGAGCAGGTGGTAATAGATGTCATCGTCGACGTAGTCGCCGGGGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCg                                                  <  8:338277/146‑1 (MQ=255)
                                                                                         gatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCgg                                                 <  2:156851/149‑1 (MQ=255)
                                                                                         gatgtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCgg                                                 >  3:18299/1‑149 (MQ=255)
                                                                                            gtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCgg                                                           >  7:300962/1‑136 (MQ=255)
                                                                                            gtcgGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCgg                                                           <  8:300962/136‑1 (MQ=255)
                                                                                               ggcggcGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGt                                           <  7:340770/149‑1 (MQ=255)
                                                                                                   gcgTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGACGATGCCGGTCAAGTCGCt                                       <  7:189949/149‑1 (MQ=255)
                                                                                                     gTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGa                                     >  3:248931/1‑149 (MQ=255)
                                                                                                      tCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGAt                                    <  4:20368/149‑1 (MQ=255)
                                                                                                           cAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACg                                >  1:106509/1‑148 (MQ=255)
                                                                                                              gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                            >  7:287267/1‑149 (MQ=255)
                                                                                                              gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                            >  8:11443/1‑149 (MQ=255)
                                                                                                              gTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTAATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCAtt                            >  3:66104/1‑149 (MQ=255)
                                                                                                               ttGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTc                           <  7:11443/149‑1 (MQ=255)
                                                                                                               ttGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTc                           >  4:307458/1‑149 (MQ=255)
                                                                                                                  tAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGAc                        >  2:272952/1‑149 (MQ=255)
                                                                                                                      aGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg                    >  7:66181/1‑149 (MQ=255)
                                                                                                                       gATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg                    <  7:339851/148‑1 (MQ=255)
                                                                                                                       gATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCg                    <  5:274225/148‑1 (MQ=255)
                                                                                                                        aTGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGt                  >  3:284482/1‑149 (MQ=255)
                                                                                                                             aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATcc             >  6:266615/1‑149 (MQ=255)
                                                                                                                             aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATcc             >  6:116754/1‑149 (MQ=255)
                                                                                                                             aTCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGAAGAAGCATGACTCGCTCATTGAGGAACTTGTCGGTAGGAGCAATCTTGACGCAGGCGATGCCGGTCAAGTCGCTGATCCGGCAGTCGACTTCGGTGATcc             >  4:286619/1‑149 (MQ=255)
                                                                                                                                gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt          <  8:66181/149‑1 (MQ=255)
                                                                                                                                gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt          >  8:253374/1‑149 (MQ=255)
                                                                                                                                gtcgACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGgt          <  5:116754/149‑1 (MQ=255)
                                                                                                                                    aCGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTc      >  4:13358/1‑149 (MQ=255)
                                                                                                                                      gTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCtt    >  4:44569/1‑149 (MQ=255)
                                                                                                                                         gTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCTTTc  <  7:253374/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
TCGAAGGTGTCGTGGATCACGATCGCGGTTGGCTCGATCGCCCACGGGATCTGGTAAACGGCGGTGGGATCGGGGCGGCAGACCATGTCGATGTCGGCGGCGTCGAGCAGGTTGTAATAGATGTCATCGTCGACGTAGTCGCCGGTGACTGTCTGCAGCAGCACGCTTTCTGGCAGACGCATGCCTCGCTCATGGAGGAACTTGGCGGTAGGCGCAATCTTGCCGCGGGCGATGCCGGTCAAGTCGCTGATCACGCATTCGACTTCGGTGATCCGGTGTTCTTTC  >  NC_002947/3566045‑3566329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑