Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,633,791	C→G	12.5%	R63R (CGC→CGG)	PP_3202 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,633,791	0	C	G	12.5%	188.9 / 19.2	72	R63R (CGC→CGG)	PP_3202	transporter
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base G (3/6);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-02

GCGCGTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCGGTAT  >  NC_002947/3633649‑3633939                                                                                                                                               |                                                                                                                                                     gcgcGTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGatcat                                                                                                                                                <  1:12885/149‑1 (MQ=255)   gcgTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATcc                                                                                                                                              <  6:164270/149‑1 (MQ=255)    cgTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCAAGAAGATGATCGCGCGGGatcatctt                                                                                                                                             <  2:16826/149‑3 (MQ=255)     gTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatcttc                                                                                                                                            <  7:221142/149‑4 (MQ=255)      tCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatcttct                                                                                                                                           <  6:142652/149‑5 (MQ=255)      tCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatcttct                                                                                                                                           <  8:197467/149‑5 (MQ=255)        cGGCTCGCTGCTGGAACTTGCGCTCTTCAACGACCGTGTGCTGGTGTTTCTGCTGGGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGGCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatcttctc                                                                                                                                          <  7:203264/148‑6 (MQ=255)        cGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatcttctc                                                                                                                                          <  7:250765/148‑6 (MQ=255)         cgCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGCGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCAGCGCGATCATCCGTTCAt                                                                                                                                        >  7:269778/2‑149 (MQ=255)          gCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATc                                                                                                                                       <  2:208534/149‑1 (MQ=255)          gCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATc                                                                                                                                       >  2:490/1‑149 (MQ=255)              gctgctGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCcaca                                                                                                                                   <  1:371753/149‑1 (MQ=255)                   tgGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTAc                                                                                                                              >  3:198409/1‑149 (MQ=255)                       aCGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTg                                                                                                                           >  4:355642/1‑148 (MQ=255)                             gcTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCAcc                                                                                                                    >  7:335220/1‑149 (MQ=255)                             gcTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCAcc                                                                                                                    >  3:182838/1‑149 (MQ=255)                                 ttCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGc                                                                                                                  >  6:19648/1‑147 (MQ=255)                                 ttCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGc                                                                                                                  >  6:319505/1‑147 (MQ=255)                                   cAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGcc                                                                                                                 <  4:308011/146‑1 (MQ=255)                                   cAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGAACCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAg                                                                                                               >  5:193743/1‑148 (MQ=255)                                         ccGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCg                                                                                                        >  8:325724/1‑149 (MQ=255)                                         ccGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCg                                                                                                        >  2:344747/1‑149 (MQ=255)                                            tgtgCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCg                                                                                                     <  6:1630/149‑1 (MQ=255)                                             gtgCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCgg                                                                                                    >  3:141182/1‑149 (MQ=255)                                                   gtgtTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTg                                                                                                <  3:297899/147‑1 (MQ=255)                                                    tgtTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGc                                                                                             <  1:490/149‑1 (MQ=255)                                                      ttGCTGCTGTGCCGGGCGGCGTCCTTGCTGCTCGGCTGGGAGGCCTCGCGCCTGAACCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCaa                                                                                           <  4:44078/149‑1 (MQ=255)                                                        gctgctGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAAcg                                                                                         >  3:43856/1‑149 (MQ=255)                                                            ctgTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGt                                                                                     <  5:151094/149‑1 (MQ=255)                                                                  cTGGCGGCGACCTTGCTGCTCGGCGGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCAt                                                                               <  5:276642/149‑1 (MQ=255)                                                                    ggcggcGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAg                                                                                                                        <  1:143484/106‑1 (MQ=255)                                                                    ggcggcGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCg                                                                             >  6:369667/1‑149 (MQ=255)                                                                    ggcggcGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCg                                                                             <  5:357530/149‑1 (MQ=255)                                                                    ggcggcGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCAAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTAATCCACAACTACCTGGAg                                                                                                                        >  2:143484/1‑106 (MQ=255)                                                                         cgACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTg                                                                        <  6:24485/149‑1 (MQ=255)                                                                          gACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTgg                                                                       <  6:348690/149‑1 (MQ=255)                                                                          gACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTgg                                                                       >  5:348690/1‑149 (MQ=255)                                                                             cTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGcc                                                                     >  5:333042/1‑148 (MQ=255)                                                                              ttGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCaa                                                                   >  8:301208/1‑149 (MQ=255)                                                                              ttGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCaa                                                                   >  7:153548/1‑149 (MQ=255)                                                                                gctgctCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAAt                                                                  <  5:109934/148‑1 (MQ=255)                                                                                     tCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCcgcgc                                                            >  3:33453/1‑149 (MQ=255)                                                                                     tCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCcgcgc                                                            >  4:153735/1‑149 (MQ=255)                                                                                       ggCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTggg                                                                                              <  3:285200/113‑1 (MQ=255)                                                                                       ggCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTggg                                                                                              >  4:285200/1‑113 (MQ=255)                                                                                              aGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATc                                                   <  5:34494/149‑1 (MQ=255)                                                                                                  cTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTa                                                 <  3:298928/147‑1 (MQ=255)                                                                                                  cTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTa                                                 >  4:298928/1‑147 (MQ=255)                                                                                                      cgcCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACaa                                           <  3:55293/149‑1 (MQ=255)                                                                                                      cgcCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACaa                                           <  4:198409/149‑1 (MQ=255)                                                                                                       gcCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAg                                          <  3:355642/149‑1 (MQ=255)                                                                                                         cTGACCCTCAATTCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGa                                        <  2:278333/149‑1 (MQ=255)                                                                                                             cccTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTAc                                    <  5:319505/149‑1 (MQ=255)                                                                                                               cTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCt                                  >  6:151311/1‑149 (MQ=255)                                                                                                               cTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGAACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCt                                  >  7:241945/1‑149 (MQ=255)                                                                                                                tCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAAc                                                                                                                                 >  1:180314/1‑53 (MQ=255)                                                                                                                tCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAAc                                                                                                                                 <  2:180314/53‑1 (MQ=255)                                                                                                                          cTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTgc                       <  6:333042/149‑1 (MQ=255)                                                                                                                             cGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTgcag                     <  3:153735/148‑1 (MQ=255)                                                                                                                             cGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTgca                      <  5:369667/147‑1 (MQ=255)                                                                                                                              gagaAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCTCCAGGAACTCGCCGGCCTGGGCAACTCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACATGCACAGCCTgcagca                   >  7:225033/1‑149 (MQ=255)                                                                                                                               agaagaTGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGCTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAACGACTACCTGCACAGCCTGcagcag                  >  5:116292/1‑149 (MQ=255)                                                                                                                                gaagaTGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCagcagc                 <  7:325724/149‑1 (MQ=255)                                                                                                                                acggatgatCGCGCGGGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCagcagc                 >  4:109750/4‑149 (MQ=255)                                                                                                                                 aagaTGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTgcag                     <  6:264999/144‑1 (MQ=255)                                                                                                                                 aagaTGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGcagcagat                >  5:264999/1‑147 (MQ=255)                                                                                                                                   gatgatCGCGCGGGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTc               >  4:279693/1‑148 (MQ=255)                                                                                                                                   gatgatCGCGCGGGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTc               >  6:117651/1‑148 (MQ=255)                                                                                                                                     tgatCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAac            <  1:344747/149‑1 (MQ=255)                                                                                                                                       atCCCGCGCGATCATCCGTTCATCCACAACTACCTGGATCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTgcgc                                                                                 <  2:337649/78‑1 (MQ=255)                                                                                                                                        tCCCGCGCGATCATCCGTTCATACACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGTACTACCTGCACAGCCTGCAGCAGCTCAacgac         <  8:269778/149‑1 (MQ=255)                                                                                                                                         cccGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAacgacg        >  6:102926/1‑149 (MQ=255)                                                                                                                                           cgcgcgATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGAcgcg      >  3:314305/1‑149 (MQ=255)                                                                                                                                            gcgcgATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCgg     >  5:14747/1‑149 (MQ=255)                                                                                                                                             cgcgATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCGGt    <  4:314305/149‑1 (MQ=255)                                                                                                                                               cgATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCGGtat  <  4:141182/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     GCGCGTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCGGTAT  >  NC_002947/3633649‑3633939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑