Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,636,403	A→T	8.4%	I111F (ATC→TTC)	PP_3203 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,636,403	0	A	T	8.4%	154.2 / 2.0	55	I111F (ATC→TTC)	PP_3203	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base T (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTG  >  NC_002947/3636256‑3636547                                                                                                                                                    |                                                                                                                                                   gCCTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCAt                                                                                                                                                   >  2:80872/1‑149 (MQ=255)   cTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTTGGGCGGCGAGCACTGCTGCTGGCTAGCATTTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTTGCTTGACTCGCTGGTGCTGATTTTCGCGAGGCGCGCCTGGATCg                                                                                                                                                 <  8:14989/149‑1 (MQ=255)          gcgcCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGTGCCGGCTTAGGccaagc                                                                                                                                          >  5:226360/1‑144 (MQ=255)          gcgcCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTgg                                                                                                                                           <  6:237568/148‑1 (MQ=255)          gcgcCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGc                                                                                                                                          >  1:97412/1‑149 (MQ=255)              cgcTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCGGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCGGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGaa                                                                                                                                      <  1:61032/149‑1 (MQ=255)                cTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGc                                                                                                                                    >  8:307601/1‑149 (MQ=255)                  ggCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCg                                                                                                                                  <  7:336752/149‑1 (MQ=255)                  ggCCGGGGGGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTGGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCg                                                                                                                                  <  7:342068/149‑1 (MQ=255)                   gCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCg                                                                                                                                  <  1:357483/148‑1 (MQ=255)                   gCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGcc                                                                                                                                   <  1:331696/147‑1 (MQ=255)                   gCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGcc                                                                                                                                   <  1:145575/147‑1 (MQ=255)                    ccGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCgg                                                                                                                                 >  4:145785/1‑148 (MQ=255)                            ttGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGAc                                                                                                                        <  4:96778/149‑1 (MQ=255)                                 aGACCGAGTGGGGCGGCGAGCACTGCTGATGGCGAGCATGTTGCTCTACAGCCTGTGTGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGtt                                                                                                                   >  5:380251/1‑149 (MQ=255)                                  gACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTg                                                                                                                  >  6:270995/1‑149 (MQ=255)                                   aCCGGGTTGGGCGGCGAGCACTTCTGGTGGCGAGCATGTTGCTCTAGATCCTTTGAGGTTTGTTGCCGTTGTTGCTGGAGTCGCGGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCTTGGGCGAAGCCGGCATCATGACCTGTTGc                                                                                                                 <  8:208693/149‑1 (MQ=255)                                    ccGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGca                                                                                                                <  6:343788/149‑1 (MQ=255)                                           gggCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGc                                                                                                          <  7:307601/148‑1 (MQ=255)                                              cggcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGAt                                                                                                      <  6:166606/149‑1 (MQ=255)                                                gcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATgg                                                                                                    >  4:182334/1‑149 (MQ=255)                                                  gAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGt                                                                                                  <  6:265110/149‑1 (MQ=255)                                                     cACTGCTGCTGGCGTGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGGTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGTAGCCGGCTTCATGACCTGTTGCACCACGCTGATGGGTGa                                                                                                <  5:297461/148‑1 (MQ=255)                                                       ctgctgctgGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGacca                                                                                             >  4:342443/1‑147 (MQ=255)                                                          ctgctgGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGactac                                                                                            <  8:136634/147‑1 (MQ=255)                                                          ctgctgGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACtt                                                                                          <  5:146494/149‑1 (MQ=255)                                                              tgGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGAc                                                                                      >  8:81616/1‑149 (MQ=255)                                                                 cGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGc                                                                                   <  1:80872/149‑1 (MQ=255)                                                                     cATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAgc                                                                               >  4:74240/1‑149 (MQ=255)                                                                        gTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑cc                                                                           >  1:70066/1‑149 (MQ=255)                                                                           gCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCgg                                                                         <  6:256091/148‑1 (MQ=255)                                                                            ctctACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCCGCATAGGCTTGGCCGAAGCCGGGATCATGGGCGGGTGGCCCCCGCGGATGGGGGGCTACTTCGACGGGCCGCG‑CCgggg                                                                       >  7:233845/1‑148 (MQ=255)                                                                             tctACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCgg                                                                                                                                 >  4:2279/1‑91 (MQ=255)                                                                             tctACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCgg                                                                                                                                 <  3:2279/91‑1 (MQ=255)                                                                                    ccTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGgttgt                                                               >  3:200084/1‑149 (MQ=255)                                                                                        tgCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTc                                                             <  3:145785/147‑1 (MQ=255)                                                                                          cGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑tt                                                        <  3:74240/149‑1 (MQ=255)                                                                                           ggTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑ttt                                                       >  8:329009/1‑149 (MQ=255)                                                                                                   gCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGAtg                                                <  2:97412/148‑1 (MQ=255)                                                                                                    ccGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGAtgg                                               <  7:281077/148‑1 (MQ=255)                                                                                                     cGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGAtggtg                                             <  4:200084/149‑1 (MQ=255)                                                                                                         gtgGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCa                                         >  3:305281/1‑149 (MQ=255)                                                                                                           ggCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCAcc                                       >  3:50846/1‑149 (MQ=255)                                                                                                                     gCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGGCTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGAtggtg                                             <  6:106200/133‑1 (MQ=255)                                                                                                                     gCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGGCTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGAtggtg                                             >  5:106200/1‑133 (MQ=255)                                                                                                                      cTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTggcggc                            >  3:141899/1‑149 (MQ=255)                                                                                                                         gTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGGTGTTCGC‑TTTGGAGATGGTGGTCACCTCAGTggcggcggc                         >  8:344583/1‑149 (MQ=255)                                                                                                                            cTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgtt                      <  5:219888/149‑1 (MQ=255)                                                                                                                             tGATTGTCGCCAGCCGCGCCGGCAAAGGGGTGGGCGGACCCGGCCTCAGGGACAGTTGGACCACGCGGTGTGGGGGCGAATTCGACGGCCCGCGCCCGGGCG‑GGGTGTTCGCGTTTGCGGTTGG‑GGTCACCACATAtggggcggggggt                     >  2:190712/1‑144 (MQ=255)                                                                                                                                   tCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAt                <  5:270995/148‑1 (MQ=255)                                                                                                                                    cGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAtgg              >  2:114770/1‑149 (MQ=255)                                                                                                                                     gCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAtgg              <  3:182334/148‑1 (MQ=255)                                                                                                                                            gcgcCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAtgggcgtgggc      >  4:176537/1‑149 (MQ=255)                                                                                                                                               ccGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGt   <  7:81616/149‑1 (MQ=255)                                                                                                                                                cGGCTTCGGCCAAGCCGATGCCGGCGTCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTg  >  7:279157/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCCTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCG‑CCGGGCGCGGTTGTTCGC‑TTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTG  >  NC_002947/3636256‑3636547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑