Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,639,337 T→C 7.2% V117A (GTG→GCG)  PP_3206 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,639,3370TC 7.2% 195.6 / 4.2 69V117A (GTG→GCG) PP_3206hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/28);  new base C (2/3);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

GCTGACGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGAGGTTGAC  >  NC_002947/3639189‑3639485
                                                                                                                                                    |                                                                                                                                                     
gCTGACGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGgt                                                                                                                                                       <  8:36898/149‑1 (MQ=255)
gCTGACGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGgt                                                                                                                                                       <  3:44999/149‑1 (MQ=255)
 cTGACGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGgtg                                                                                                                                                      >  2:273360/1‑149 (MQ=255)
    aCGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGgtgt                                                                                                                                                     <  1:376509/147‑1 (MQ=255)
    aCGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTa                                                                                                                                                    <  3:350479/148‑1 (MQ=255)
    aCGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTAc                                                                                                                                                   <  3:369897/149‑1 (MQ=255)
        tgtCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGc                                                                                                                                                 >  8:186092/1‑147 (MQ=255)
        tgtCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGc                                                                                                                                                 >  6:145367/1‑147 (MQ=255)
        tgtCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGCGTACACca                                                                                                                                               <  8:117316/149‑1 (MQ=255)
            gATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCcag‑cag                                                                                                                                          >  7:278999/1‑149 (MQ=255)
                 ggTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTcg                                                                                                                                     >  6:203650/1‑149 (MQ=255)
                  gTCTTCCATCTCGTCAGTGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTcg                                                                                                                                     <  5:97923/148‑1 (MQ=255)
                  gTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTcgc                                                                                                                                    >  8:332549/1‑149 (MQ=255)
                       ccATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTAACCAGGTAAACCTGCAAGCAAGCCTGGAGATGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCCgtg                                                                                                                                >  5:219965/1‑148 (MQ=255)
                         aTCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTAc                                                                                                                                                   <  4:317752/128‑1 (MQ=255)
                          tctcGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTAcg                                                                                                                            >  5:182186/1‑149 (MQ=255)
                           ctcGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTAcgg                                                                                                                           >  3:165818/1‑149 (MQ=255)
                            tcGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTAcggc                                                                                                                          >  8:322206/1‑149 (MQ=255)
                              gTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCGTCTGTCCACAGTTGCTGGTGTACGCCAG‑CAGCGTCGCTGTGTAcggcgg                                                                                                                        <  8:354872/149‑1 (MQ=255)
                              gTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAAGCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTAcggagg                                                                                                                        >  8:127009/1‑149 (MQ=255)
                               tCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTGACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACggcggc                                                                                                                       <  7:75088/149‑1 (MQ=255)
                                 aGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCaa                                                                                                                     <  8:208829/149‑1 (MQ=255)
                                    gTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGtt                                                                                                                  <  5:145367/149‑1 (MQ=255)
                                       ccTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGcc                                                                                                               >  5:36734/1‑149 (MQ=255)
                                       ccTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGc                                                                                                                <  1:158150/148‑1 (MQ=255)
                                        cTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCg                                                                                                              >  8:16672/1‑149 (MQ=255)
                                         tGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGa                                                                                                             >  1:74090/1‑149 (MQ=255)
                                          ggcggcGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGAc                                                                                                            >  5:111163/1‑149 (MQ=255)
                                              gcgcTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTTGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACgcgc                                                                                                        <  2:74090/149‑1 (MQ=255)
                                                 cTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATg                                                                                                     <  3:94013/149‑1 (MQ=255)
                                                  tGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGTTGTACGGCGGCAAGTTGCCGACGCGCATgg                                                                                                    >  3:302147/1‑149 (MQ=255)
                                                    ccGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATgg                                                                                                    <  4:165818/147‑1 (MQ=255)
                                                        gaCGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAg                                                                                              >  2:132460/1‑149 (MQ=255)
                                                          cggattACGAACGGGGGTTCCATGTAAACCTGGAAGGAATGCTGGATCTGCTCGTACAACTTCGTAATCCCCTCTTTCCACCGGTGGTGGTGTTAGCCAGTCAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAg                                                                                              <  1:273360/143‑1 (MQ=255)
                                                           gCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGgc                                                                                            >  6:321835/1‑148 (MQ=255)
                                                                  gAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCcagccagc                                                                                    >  8:109686/1‑149 (MQ=255)
                                                                    aCGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGt                                                                                  >  2:69936/1‑149 (MQ=255)
                                                                                 gTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTc                                                                     <  7:186092/149‑1 (MQ=255)
                                                                                 gTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTc                                                                     <  7:332549/149‑1 (MQ=255)
                                                                                    aaCCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTa                                                                  >  1:2688/1‑149 (MQ=255)
                                                                                     aCCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTAt                                                                 >  4:111734/1‑149 (MQ=255)
                                                                                      ccTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATg                                                                >  4:320117/1‑149 (MQ=255)
                                                                                       cTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATgc                                                               >  8:112932/1‑149 (MQ=255)
                                                                                             gcaagcCGTGAGCTGCTCGACCAACTGCGTAATCCCCTCTGGCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGca                                                         <  6:125299/149‑1 (MQ=255)
                                                                                                 gcCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACaaa                                                     >  8:153145/1‑149 (MQ=255)
                                                                                                  ccTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAAc                                                    <  2:2688/149‑1 (MQ=255)
                                                                                                  ccTGGAGCTGCTCGACCAACTGCGTAATCACCTCTGTCCACCGGTGCTGGTCTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAACTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAAc                                                    >  5:62666/1‑149 (MQ=255)
                                                                                                      gAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCAt                                                <  4:333539/149‑1 (MQ=255)
                                                                                                       aGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCAtg                                               <  2:250333/149‑1 (MQ=255)
                                                                                                            ctCGACCAACTGCTTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGTCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTGCTATGCGGCGCACAAACGCAttgtg                                            <  6:219965/147‑1 (MQ=255)
                                                                                                            ctCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGga                                          <  8:91695/149‑1 (MQ=255)
                                                                                                                   aaCTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATcgcg                                   >  4:39822/1‑149 (MQ=255)
                                                                                                                      tGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTg                                >  4:256949/1‑149 (MQ=255)
                                                                                                                         gTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAg                             <  7:16672/149‑1 (MQ=255)
                                                                                                                           aaTCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAgg                            <  6:36734/148‑1 (MQ=255)
                                                                                                                               cccTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCtt                       >  8:234705/1‑149 (MQ=255)
                                                                                                                                ccTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTg                      >  1:244730/1‑149 (MQ=255)
                                                                                                                                 ctctGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACACACGCATGGTGGAGATCGCGTTGCAGGATCTTGc                     >  2:238318/1‑149 (MQ=255)
                                                                                                                                 ctctGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGc                     >  1:160852/1‑149 (MQ=255)
                                                                                                                                    tGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCTTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCccg                  >  1:13686/1‑149 (MQ=255)
                                                                                                                                        cACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCccgccg               <  6:111163/148‑1 (MQ=255)
                                                                                                                                        cACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGt              >  2:30421/1‑149 (MQ=255)
                                                                                                                                         gacgctGCTGGCGTACACCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATc                         >  2:349822/6‑137 (MQ=255)
                                                                                                                                         gacgctGCTGGCGTACACCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATc                         <  1:349822/132‑1 (MQ=255)
                                                                                                                                          ccGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTgg            <  3:39822/149‑1 (MQ=255)
                                                                                                                                            gctGCTGGCGTACACCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTg             <  3:22716/144‑1 (MQ=255)
                                                                                                                                            gctGCTGGCGTACACCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTg             >  4:22716/3‑146 (MQ=255)
                                                                                                                                             gTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGa         <  7:127009/149‑1 (MQ=255)
                                                                                                                                              tGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCAtgg                                              <  8:227861/111‑1 (MQ=255)
                                                                                                                                              tGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCAtgg                                              >  7:227861/1‑111 (MQ=255)
                                                                                                                                              tGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGAg        >  4:72335/1‑149 (MQ=255)
                                                                                                                                               gCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGAgg       <  7:153145/149‑1 (MQ=255)
                                                                                                                                                 tGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGAGGtt     >  6:123943/1‑149 (MQ=255)
                                                                                                                                                    tgtACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGAGGTTGAc  <  7:109686/149‑1 (MQ=255)
                                                                                                                                                    tgtACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGAGGTTGAc  <  5:203650/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTGACGGTGTCGATGTGGTCTTCCATCTCGTCAGCGTGCCTGGCGGCGCTGCCGAGACGCAGTACGAACGGGGTTACCAGGTAAACCTGCAAGCAAGCCTGGAGCTGCTCGACCAACTGCGTAATCCCCTCTGTCCACCGGTGCTGGTGTACGCCAG‑CAGCGTCGCGGTGTACGGCGGCAAGTTGCCGACGCGCATGGACGAAGGCCAGCCAGCGTCGCCGCAGCTGTCCTATGCGGCGCACAAACGCATGGTGGAGATCGCGTTGCAGGATCTTGCCCGCCGTGGCGAGGTTGAC  >  NC_002947/3639189‑3639485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑