Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,647,235 T→G 7.7% intergenic (‑172/‑482) PP_3213 ← / → PP_3214 ABC transporter substrate‑binding protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,647,2350TG 7.7% 148.9 / 4.0 52intergenic (‑172/‑482)PP_3213/PP_3214ABC transporter substrate‑binding protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/29);  new base G (2/2);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

ACCGTGCGCCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGCGACCCGCACCCGAGGG  >  NC_002947/3647091‑3647379
                                                                                                                                                |                                                                                                                                                
accGTGCGCCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGATTGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATTGCCTCGTTCAAACATCCGCTCGGa                                                                                                                                              <  6:340023/149‑1 (MQ=255)
accGTGCGCCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGa                                                                                                                                              <  7:261190/149‑1 (MQ=255)
   gTGCGGCGGATAGCGAGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGATGAGAATTGCGTCGCGATGCAAATGGGCGTGAACTGGAAAATGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGt                                                                                                                                           <  5:365975/149‑1 (MQ=255)
   gTGCGCCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGt                                                                                                                                           <  4:248542/149‑1 (MQ=255)
     gctccGGATAGCTCGACAGCGAAGGAGCCGGGCGGAGACCACGTGGGAAGAGAATGGCGTCGCGAAGCAAACGGGCGTGAACTGGAACCTGACCTTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTAc                                                                                                                                         <  6:261614/146‑1 (MQ=255)
      cgcCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACg                                                                                                                                        >  5:202324/1‑149 (MQ=255)
      cgcCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGAGCGGATGttt                                                                                                                                         <  7:268898/148‑3 (MQ=255)
       gcCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGc                                                                                                                                       <  3:268479/149‑1 (MQ=255)
         cGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGccc                                                                                                                                     <  3:149109/149‑1 (MQ=255)
                cgcgACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGc                                                                                                                                       <  8:278942/140‑1 (MQ=255)
                cgcgACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGc                                                                                                                                       >  7:278942/1‑140 (MQ=255)
                        cGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATAGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAagag                                                                                                                      >  6:238309/1‑149 (MQ=255)
                         gAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGAGCGGATGtttgaa                                                                                                                                      <  6:27325/132‑6 (MQ=255)
                         gAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGAGCGGATGtttgaa                                                                                                                                      >  5:27325/1‑127 (MQ=255)
                                 cGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGccc                                                                                                              >  6:121130/1‑148 (MQ=255)
                                 cGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGccc                                                                                                              >  3:326521/1‑148 (MQ=255)
                                             aCGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGTAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCcatgccatgc                                                                                                    >  1:266415/1‑146 (MQ=255)
                                             aCGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCcatgccatgc                                                                                                    <  2:266415/146‑1 (MQ=255)
                                                  ggAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACt                                                                                            >  4:128368/1‑149 (MQ=255)
                                                    aaGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGa                                                                                          >  2:6603/1‑149 (MQ=255)
                                                     agagAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGAc                                                                                         <  3:128368/149‑1 (MQ=255)
                                                         aaTGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCGCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCt                                                                                     <  1:42281/149‑1 (MQ=255)
                                                          aTGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCtt                                                                                    <  5:383128/149‑1 (MQ=255)
                                                             gCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCGCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTa                                                                                 <  4:161841/149‑1 (MQ=255)
                                                             gCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCt                                                                                  <  7:347138/148‑1 (MQ=255)
                                                                  gcgAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGc                                                                            <  5:121130/149‑1 (MQ=255)
                                                                    gAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCt                                                                           >  6:285449/1‑148 (MQ=255)
                                                                     aaGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCTTAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGa                                                                         <  1:309139/149‑1 (MQ=255)
                                                                     aaGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGa                                                                         >  7:37948/1‑149 (MQ=255)
                                                                         aaaaGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATAc                                                                      >  4:351524/1‑148 (MQ=255)
                                                                           aaGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATAc                                                                      >  1:15112/1‑146 (MQ=255)
                                                                             gggCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACaagaa                                                                 <  5:238309/149‑1 (MQ=255)
                                                                                    aaCTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGaa                                                          <  2:15112/149‑1 (MQ=255)
                                                                                     aCTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGaa                                                          <  3:205828/148‑1 (MQ=255)
                                                                                     aCTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAg                                                         <  7:208554/149‑1 (MQ=255)
                                                                                       tGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCa                                                       >  1:351950/1‑149 (MQ=255)
                                                                                         gAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCAt                                                      <  1:164528/148‑1 (MQ=255)
                                                                                         gAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCAt                                                      <  7:295721/148‑1 (MQ=255)
                                                                                         gAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCAt                                                      <  4:326521/148‑1 (MQ=255)
                                                                                         gAAACTGACATTGCGCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATg                                                     <  4:201671/149‑1 (MQ=255)
                                                                                                     gCTCTGTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATg                                          >  7:340404/1‑148 (MQ=255)
                                                                                                         tctTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGAATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGcc                                     >  6:30465/1‑149 (MQ=255)
                                                                                                             gCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCt                                  >  5:181852/1‑148 (MQ=255)
                                                                                                                        ccATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGgtg                      >  1:25330/1‑149 (MQ=255)
                                                                                                                          aTAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGgtg                      >  4:306097/1‑147 (MQ=255)
                                                                                                                             gCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGc                  <  1:168559/148‑1 (MQ=255)
                                                                                                                               cTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGCGAc               <  5:285449/149‑1 (MQ=255)
                                                                                                                                  gTTCAAACATCCGCTCGGATGTACTCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGCGAcc              <  5:299453/147‑1 (MQ=255)
                                                                                                                                    gcgtaCATCCGAGCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGCGACCCGCa          >  7:46065/5‑149 (MQ=255)
                                                                                                                                      aaaCATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCAGGCACCGGTAATACACCATCAAAAAACAAGCAAAAACCATGCAAATTTCATGAAGCCGCTGAGTTTGCGTTTTAGCGACCGCCAcc        >  6:210993/1‑149 (MQ=255)
                                                                                                                                             cGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGCGACCCGCACCCGAggg  <  2:25330/148‑1 (MQ=255)
                                                                                                                                             cGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGCGACCCGCACCCGAggg  <  1:6603/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACCGTGCGCCGGATAGCGCGACAGCGAAGGAGCCGGGCGGAGAGAACGTGGGAAGAGAATGGCGTCGCGAAGCAAAAGGGCGTGAACTGGAAACTGACATTGCTCTCTTGCCCGGGGCTGCCATAGCCTCGTTCAAACATCCGCTCGGATGTACGCCCCGAAGGACGCAAGAGCAATGCCCCATGCCATGCCTGGCACTGACCGCTTCTACTCGCTGATACAAGAAGCCAGAAGCATGCCAATTTCATGAAGCCGCTGACTTTGCGGTGTAGCGACCCGCACCCGAGGG  >  NC_002947/3647091‑3647379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑