Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,701,990	T→G	10.1%	Y140* (TAT→TAG)	PP_3267 →	Clp protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,701,990	0	T	G	10.1%	163.9 / 7.1	57	Y140* (TAT→TAG)	PP_3267	Clp protease
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base G (4/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGTC  >  NC_002947/3701848‑3702136                                                                                                                                                          |                                                                                                                                                    tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                                  >  1:352911/1‑146 (MQ=255) tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                                  >  3:147981/1‑146 (MQ=255)   ttCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCAGGATTACGACCTGCGCCTGTATgcg                                                                                                                                                  >  5:274815/1‑144 (MQ=255)       ttGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTg                                                                                                                                         >  8:179747/1‑149 (MQ=255)       ttGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTg                                                                                                                                         <  4:147981/149‑1 (MQ=255)         gCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTg                                                                                                                                         <  5:351229/147‑1 (MQ=255)            ggAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTAAGAGGGGGCCTGATAGAGAGCGGATGTCCGAATATGAGATGAGCCTGGATTACGACCTGCGCCTTTATgcg                                                                                                                                                  >  1:118810/1‑146 (MQ=255)                   gACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCAc                                                                                                                              >  6:87933/1‑148 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTgg                                                                                                                                        >  5:42530/1‑133 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTgg                                                                                                                                        <  6:42530/133‑1 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAg                                                                                                                         >  3:97146/1‑148 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGACGGAGCGCACGAAAgg                                                                                                                        >  7:98742/1‑149 (MQ=255)                           gCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGgcgc                                                                                                                     <  4:168574/149‑1 (MQ=255)                           gCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGgcgc                                                                                                                     <  2:352911/149‑1 (MQ=255)                                cAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGcc                                                                                                                 >  8:156204/1‑148 (MQ=255)                                 aGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGAGGAGCGCACGAAATGCGCGGCCGa                                                                                                               <  6:381504/149‑1 (MQ=255)                                  gTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCTTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAg                                                                                                              <  7:87254/149‑1 (MQ=255)                                   tttCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGaaa                                                                                                                          <  3:199897/136‑1 (MQ=255)                                   tttCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGaaa                                                                                                                          >  4:199897/1‑136 (MQ=255)                                        gCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑g                                                                                                       >  2:97785/1‑149 (MQ=255)                                                      cccccGTATCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCGCGATTACGACCTGCGCCTGTATGAGCAGATCGAGGCGTAGCCCACGAAAGGCGCGTCCGAGGGTTT‑GGATGTACCGCGTTa                                                                                         >  2:153368/3‑149 (MQ=255)                                                      cACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTa                                                                                         <  1:274579/149‑1 (MQ=255)                                                       aCCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTAt                                                                                        <  7:156204/149‑1 (MQ=255)                                                            tttCAATGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAAt                                                                                   <  4:97146/149‑1 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGcc                                                                               >  5:212386/1‑149 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGc                                                                                <  2:357803/148‑1 (MQ=255)                                                                          gCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGc                                                                                <  2:105115/138‑1 (MQ=255)                                                                          gCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGc                                                                                >  1:105115/1‑138 (MQ=255)                                                                            aTGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATa                                                                   >  5:266646/1‑149 (MQ=255)                                                                             tGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATAc                                                                  <  1:250076/149‑1 (MQ=255)                                                                                              aCC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                       >  4:104413/1‑149 (MQ=255)                                                                                                c‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                       >  7:262145/1‑147 (MQ=255)                                                                                                   aT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAg                                                    <  1:52810/149‑1 (MQ=255)                                                                                                          gcgGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGgcg                                                 <  5:87933/148‑1 (MQ=255)                                                                                                                   cGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                                       <  2:118810/149‑1 (MQ=255)                                                                                                                   cGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGACGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                                       <  8:98742/149‑1 (MQ=255)                                                                                                                   cGAGTATGCGATGAGCCTGGAGTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                                       <  2:123350/149‑1 (MQ=255)                                                                                                                    gAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                      <  7:179747/149‑1 (MQ=255)                                                                                                                      gTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGga                                    <  5:282641/149‑1 (MQ=255)                                                                                                                            gATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                       <  1:219405/124‑1 (MQ=255)                                                                                                                            gATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                       >  2:219405/1‑124 (MQ=255)                                                                                                                            gATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATc                               >  5:147860/1‑148 (MQ=255)                                                                                                                            gATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATc                               >  5:61096/1‑148 (MQ=255)                                                                                                                              tGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCAt                            <  1:97785/149‑1 (MQ=255)                                                                                                                                       aTTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   <  6:61096/149‑1 (MQ=255)                                                                                                                                         caCGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   >  8:106957/2‑147 (MQ=255)                                                                                                                                         caCGATCTGCGCATACAGGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAg                                                    <  6:201908/113‑1 (MQ=255)                                                                                                                                         caCGATCTGCGCATACAGGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAg                                                    >  5:201908/2‑114 (MQ=255)                                                                                                                                          aCGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   >  2:36704/1‑146 (MQ=255)                                                                                                                                           cGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   >  8:307491/1‑145 (MQ=255)                                                                                                                                           cGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGGTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   >  7:124442/1‑145 (MQ=255)                                                                                                                                            gcccggcgcCTGTAGGCGCAGATCCTGGCGGAGCGCACGAAAGGCGCGGCCGCGGGTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGAt              <  4:272539/144‑1 (MQ=255)                                                                                                                                              ccTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGc            <  7:296726/149‑1 (MQ=255)                                                                                                                                                     cTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATcgc     >  5:84420/1‑149 (MQ=255)                                                                                                                                                        tATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATcgcct   >  1:83092/1‑147 (MQ=255)                                                                                                                                                        tATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGCCGAGATGCCGATCTCGt   >  2:129679/1‑148 (MQ=255)                                                                                                                                                        tATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGTc  <  6:212386/149‑1 (MQ=255)                                                                                                                                                          |                                                                                                                                                    TCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGT‑‑‑‑‑‑GGACC‑‑AT‑‑‑TCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTT‑GGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGTC  >  NC_002947/3701848‑3702136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑