Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,704,986 A→T 6.8% L339Q (CTG→CAG)  paaZ ← bifunctional oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,704,9860AT 6.8% 173.3 / 2.5 59L339Q (CTG→CAG) paaZbifunctional oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (26/29);  new base T (2/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

TGCAACAAGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTG  >  NC_002947/3704839‑3705132
                                                                                                                                                   |                                                                                                                                                  
tGCAACAAGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAg                                                                                                                                                   >  6:174782/1‑149 (MQ=255)
  caacaaGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAgg                                                                                                                                                  <  3:361598/148‑1 (MQ=255)
   aacaaGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAgg                                                                                                                                                  >  8:3755/1‑147 (MQ=255)
   aacaaGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGgc                                                                                                                                                >  5:333736/1‑149 (MQ=255)
    acaaGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGgcg                                                                                                                                               >  4:118758/1‑149 (MQ=255)
        ggTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCa                                                                                                                                           >  4:178603/1‑149 (MQ=255)
          ttGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATg                                                                                                                                         <  3:178603/149‑1 (MQ=255)
             gcgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATgcgc                                                                                                                                      <  6:333736/149‑1 (MQ=255)
             gcgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATgcgc                                                                                                                                      <  5:310004/149‑1 (MQ=255)
              cgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacca                                                                                                                                     <  1:157983/149‑5 (MQ=255)
               gcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacc                                                                                                                                      >  3:278897/1‑144 (MQ=255)
               gcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacc                                                                                                                                      <  4:278897/147‑4 (MQ=255)
                   aGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCt                                                                                                                                <  2:219948/149‑1 (MQ=255)
                     aaCGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCtt                                                                                                                               >  3:151095/1‑148 (MQ=255)
                       cgcgCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCaa                                                                                                                            <  5:174782/149‑1 (MQ=255)
                            cTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGc                                                                                                                          <  7:91902/146‑1 (MQ=255)
                            cTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGc                                                                                                                          >  8:91902/1‑146 (MQ=255)
                                       gcTTTGCCCCGCGGGGCGAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAAc                                                                                                            >  3:351496/2‑149 (MQ=255)
                                       ccTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAAc                                                                                                            >  3:247622/1‑149 (MQ=255)
                                          tCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCAc                                                                                                         >  4:335510/1‑149 (MQ=255)
                                          tCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAAGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCAc                                                                                                         >  1:123623/1‑149 (MQ=255)
                                             ccccGCGGGGCAAAGCGATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGGGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAc                                                                                                      <  3:351975/149‑1 (MQ=255)
                                             ccccGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAc                                                                                                      <  8:247894/149‑1 (MQ=255)
                                              cccGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAcc                                                                                                     >  7:257422/1‑149 (MQ=255)
                                                 gcgGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTg                                                                                                  >  1:183937/1‑149 (MQ=255)
                                                   ggggCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCt                                                                                                <  7:3755/149‑1 (MQ=255)
                                                      gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                             >  1:110552/1‑149 (MQ=255)
                                                      gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                             <  2:123623/149‑1 (MQ=255)
                                                      gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                             <  2:66471/149‑1 (MQ=255)
                                                       cAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAgg                                                                                                                                                  <  6:373698/95‑1 (MQ=255)
                                                       cAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAgg                                                                                                                                                  >  5:373698/1‑95 (MQ=255)
                                                       cAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAgg                                                                                            <  5:27144/149‑1 (MQ=255)
                                                              aTCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgc                                                                                     <  7:305252/149‑1 (MQ=255)
                                                              aTCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgc                                                                                     <  6:43514/149‑1 (MQ=255)
                                                               tCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgcg                                                                                    <  8:338911/149‑1 (MQ=255)
                                                                 gCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCgcgc                                                                                   <  8:257422/148‑1 (MQ=255)
                                                                  cTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAg                                                                                 >  1:357004/1‑149 (MQ=255)
                                                                      cgcCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCgg                                                                             >  5:138925/1‑149 (MQ=255)
                                                                      cgcCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCgg                                                                             <  3:118758/149‑1 (MQ=255)
                                                                                 tGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcg                                                                                      <  1:69045/129‑1 (MQ=255)
                                                                                 tGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcg                                                                                      >  2:69045/1‑129 (MQ=255)
                                                                                   gTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACCCCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCAt                                                                >  8:317226/1‑149 (MQ=255)
                                                                                     cacaACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCa                                                                                                          <  1:366787/105‑1 (MQ=255)
                                                                                     cacaACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCa                                                                                                          >  2:366787/1‑105 (MQ=255)
                                                                                           tctGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                        >  4:46497/1‑149 (MQ=255)
                                                                                           tctGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                        >  8:1201/1‑149 (MQ=255)
                                                                                           tctGCAGCAGGCTGCGCACCCGCTCGCCGACATAGCGCTGCTGCTCAAGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTAGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                        >  8:348182/1‑149 (MQ=255)
                                                                                                     gCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGa                                              <  2:110552/149‑1 (MQ=255)
                                                                                                             cccGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCa                                      <  4:247622/149‑1 (MQ=255)
                                                                                                                gCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACgg                                   <  2:357004/149‑1 (MQ=255)
                                                                                                                      cGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATg                             >  4:14084/1‑149 (MQ=255)
                                                                                                                       gACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATgg                            <  3:335510/149‑1 (MQ=255)
                                                                                                                         cATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCg                          <  4:351496/149‑1 (MQ=255)
                                                                                                                                  tgtgcgcATGGGCGCCCTGGCCTCCCATGCGCACACCTTCAAGCGACGGGTCGCCACCCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCT‑GCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCt                >  6:103387/7‑149 (MQ=255)
                                                                                                                                   ctgGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTc                 <  4:151095/148‑1 (MQ=255)
                                                                                                                                   ctgATCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCt                >  6:163713/1‑149 (MQ=255)
                                                                                                                                              aGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTa     <  3:46497/149‑1 (MQ=255)
                                                                                                                                                gCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGt   >  2:70569/1‑149 (MQ=255)
                                                                                                                                                 ccAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTGAGGGGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGGCCGGCTTTAGTg  <  1:201076/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCAACAAGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTG  >  NC_002947/3704839‑3705132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑