Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,704,989 G→C 7.7% A338G (GCC→GGC)  paaZ ← bifunctional oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,704,9890GC 7.7% 152.9 / 2.8 52A338G (GCC→GGC) paaZbifunctional oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (21/27);  new base C (2/2);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

AACAAGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGTCA  >  NC_002947/3704842‑3705136
                                                                                                                                                   |                                                                                                                                                   
aacaaGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGgc                                                                                                                                                    >  5:333736/1‑149 (MQ=255)
 acaaGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGgcg                                                                                                                                                   >  4:118758/1‑149 (MQ=255)
     ggTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCa                                                                                                                                               >  4:178603/1‑149 (MQ=255)
       ttGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATg                                                                                                                                             <  3:178603/149‑1 (MQ=255)
          gcgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATgcgc                                                                                                                                          <  6:333736/149‑1 (MQ=255)
          gcgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATgcgc                                                                                                                                          <  5:310004/149‑1 (MQ=255)
           cgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacca                                                                                                                                         <  1:157983/149‑5 (MQ=255)
            gcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacc                                                                                                                                          >  3:278897/1‑144 (MQ=255)
            gcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacc                                                                                                                                          <  4:278897/147‑4 (MQ=255)
                aGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCt                                                                                                                                    <  2:219948/149‑1 (MQ=255)
                  aaCGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCtt                                                                                                                                   >  3:151095/1‑148 (MQ=255)
                    cgcgCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCaa                                                                                                                                <  5:174782/149‑1 (MQ=255)
                         cTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGc                                                                                                                              >  8:91902/1‑146 (MQ=255)
                         cTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGc                                                                                                                              <  7:91902/146‑1 (MQ=255)
                                    gcTTTGCCCCGCGGGGCGAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAAc                                                                                                                >  3:351496/2‑149 (MQ=255)
                                    ccTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAAc                                                                                                                >  3:247622/1‑149 (MQ=255)
                                       tCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCAc                                                                                                             >  4:335510/1‑149 (MQ=255)
                                       tCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAAGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCAc                                                                                                             >  1:123623/1‑149 (MQ=255)
                                          ccccGCGGGGCAAAGCGATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGGGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAc                                                                                                          <  3:351975/149‑1 (MQ=255)
                                          ccccGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAc                                                                                                          <  8:247894/149‑1 (MQ=255)
                                           cccGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAcc                                                                                                         >  7:257422/1‑149 (MQ=255)
                                              gcgGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTg                                                                                                      >  1:183937/1‑149 (MQ=255)
                                                ggggCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCt                                                                                                    <  7:3755/149‑1 (MQ=255)
                                                   gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                                 >  1:110552/1‑149 (MQ=255)
                                                   gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                                 <  2:123623/149‑1 (MQ=255)
                                                   gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                                 <  2:66471/149‑1 (MQ=255)
                                                    cAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAgg                                                                                                <  5:27144/149‑1 (MQ=255)
                                                           aTCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgc                                                                                         <  6:43514/149‑1 (MQ=255)
                                                           aTCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgc                                                                                         <  7:305252/149‑1 (MQ=255)
                                                            tCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgcg                                                                                        <  8:338911/149‑1 (MQ=255)
                                                              gCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCgcgc                                                                                       <  8:257422/148‑1 (MQ=255)
                                                               cTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAg                                                                                     >  1:357004/1‑149 (MQ=255)
                                                                   cgcCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCgg                                                                                 >  5:138925/1‑149 (MQ=255)
                                                                   cgcCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCgg                                                                                 <  3:118758/149‑1 (MQ=255)
                                                                              tGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcg                                                                                          >  2:69045/1‑129 (MQ=255)
                                                                              tGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcg                                                                                          <  1:69045/129‑1 (MQ=255)
                                                                                gTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACCCCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCAt                                                                    >  8:317226/1‑149 (MQ=255)
                                                                                  cacaACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCa                                                                                                              >  2:366787/1‑105 (MQ=255)
                                                                                  cacaACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCa                                                                                                              <  1:366787/105‑1 (MQ=255)
                                                                                        tctGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                            >  4:46497/1‑149 (MQ=255)
                                                                                        tctGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                            >  8:1201/1‑149 (MQ=255)
                                                                                        tctGCAGCAGGCTGCGCACCCGCTCGCCGACATAGCGCTGCTGCTCAAGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTAGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                            >  8:348182/1‑149 (MQ=255)
                                                                                                  gCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGa                                                  <  2:110552/149‑1 (MQ=255)
                                                                                                          cccGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCa                                          <  4:247622/149‑1 (MQ=255)
                                                                                                             gCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACgg                                       <  2:357004/149‑1 (MQ=255)
                                                                                                                   cGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATg                                 >  4:14084/1‑149 (MQ=255)
                                                                                                                    gACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATgg                                <  3:335510/149‑1 (MQ=255)
                                                                                                                      cATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCg                              <  4:351496/149‑1 (MQ=255)
                                                                                                                               tgtgcgcATGGGCGCCCTGGCCTCCCATGCGCACACCTTCAAGCGACGGGTCGCCACCCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCT‑GCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCt                    >  6:103387/7‑149 (MQ=255)
                                                                                                                                ctgGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTc                     <  4:151095/148‑1 (MQ=255)
                                                                                                                                ctgATCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCt                    >  6:163713/1‑149 (MQ=255)
                                                                                                                                           aGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTa         <  3:46497/149‑1 (MQ=255)
                                                                                                                                             gCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGt       >  2:70569/1‑149 (MQ=255)
                                                                                                                                              ccAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTGAGGGGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGGCCGGCTTTAGTg      <  1:201076/149‑1 (MQ=255)
                                                                                                                                                 gggCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGt    >  1:106198/1‑148 (MQ=255)
                                                                                                                                                  ggCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGtgc  >  6:362561/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACAAGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGTCA  >  NC_002947/3704842‑3705136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑