Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,704,991 G→T 7.4% G337G (GGC→GGA)  paaZ ← bifunctional oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,704,9910GT 7.4% 165.6 / 3.6 54G337G (GGC→GGA) paaZbifunctional oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base T (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

ACAAGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGTCA  >  NC_002947/3704843‑3705136
                                                                                                                                                    |                                                                                                                                                 
acaaGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGgcg                                                                                                                                                   >  4:118758/1‑149 (MQ=255)
    ggTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCa                                                                                                                                               >  4:178603/1‑149 (MQ=255)
      ttGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATg                                                                                                                                             <  3:178603/149‑1 (MQ=255)
         gcgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATgcgc                                                                                                                                          <  6:333736/149‑1 (MQ=255)
         gcgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATgcgc                                                                                                                                          <  5:310004/149‑1 (MQ=255)
          cgcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacca                                                                                                                                         <  1:157983/149‑5 (MQ=255)
           gcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacc                                                                                                                                          >  3:278897/1‑144 (MQ=255)
           gcAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGCGCCCTGGCCTCCCATgacc                                                                                                                                          <  4:278897/147‑4 (MQ=255)
               aGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCt                                                                                                                                    <  2:219948/149‑1 (MQ=255)
                 aaCGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCtt                                                                                                                                   >  3:151095/1‑148 (MQ=255)
                   cgcgCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCaa                                                                                                                                <  5:174782/149‑1 (MQ=255)
                        cTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGc                                                                                                                              >  8:91902/1‑146 (MQ=255)
                        cTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGc                                                                                                                              <  7:91902/146‑1 (MQ=255)
                                   gcTTTGCCCCGCGGGGCGAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAAc                                                                                                                >  3:351496/2‑149 (MQ=255)
                                   ccTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAAc                                                                                                                >  3:247622/1‑149 (MQ=255)
                                      tCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCAc                                                                                                             >  4:335510/1‑149 (MQ=255)
                                      tCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAAGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCAc                                                                                                             >  1:123623/1‑149 (MQ=255)
                                         ccccGCGGGGCAAAGCGATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGGGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAc                                                                                                          <  3:351975/149‑1 (MQ=255)
                                         ccccGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAc                                                                                                          <  8:247894/149‑1 (MQ=255)
                                          cccGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGAcc                                                                                                         >  7:257422/1‑149 (MQ=255)
                                             gcgGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTg                                                                                                      >  1:183937/1‑149 (MQ=255)
                                               ggggCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCt                                                                                                    <  7:3755/149‑1 (MQ=255)
                                                  gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                                 >  1:110552/1‑149 (MQ=255)
                                                  gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                                 <  2:123623/149‑1 (MQ=255)
                                                  gCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAg                                                                                                 <  2:66471/149‑1 (MQ=255)
                                                   cAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAgg                                                                                                <  5:27144/149‑1 (MQ=255)
                                                          aTCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgc                                                                                         <  7:305252/149‑1 (MQ=255)
                                                          aTCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgc                                                                                         <  6:43514/149‑1 (MQ=255)
                                                           tCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcgcg                                                                                        <  8:338911/149‑1 (MQ=255)
                                                             gCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCgcgc                                                                                       <  8:257422/148‑1 (MQ=255)
                                                              cTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAg                                                                                     >  1:357004/1‑149 (MQ=255)
                                                                  cgcCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCgg                                                                                 >  5:138925/1‑149 (MQ=255)
                                                                  cgcCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCgg                                                                                 <  3:118758/149‑1 (MQ=255)
                                                                             tGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcg                                                                                          >  2:69045/1‑129 (MQ=255)
                                                                             tGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTcg                                                                                          <  1:69045/129‑1 (MQ=255)
                                                                               gTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACCCCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCAt                                                                    >  8:317226/1‑149 (MQ=255)
                                                                                 cacaACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCa                                                                                                              >  2:366787/1‑105 (MQ=255)
                                                                                 cacaACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCa                                                                                                              <  1:366787/105‑1 (MQ=255)
                                                                                       tctGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                            >  4:46497/1‑149 (MQ=255)
                                                                                       tctGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                            >  8:1201/1‑149 (MQ=255)
                                                                                       tctGCAGCAGGCTGCGCACCCGCTCGCCGACATAGCGCTGCTGCTCAAGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTAGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGc                                                            >  8:348182/1‑149 (MQ=255)
                                                                                                 gCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGa                                                  <  2:110552/149‑1 (MQ=255)
                                                                                                         cccGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCa                                          <  4:247622/149‑1 (MQ=255)
                                                                                                            gCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACgg                                       <  2:357004/149‑1 (MQ=255)
                                                                                                                  cGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATg                                 >  4:14084/1‑149 (MQ=255)
                                                                                                                   gACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATgg                                <  3:335510/149‑1 (MQ=255)
                                                                                                                     cATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCg                              <  4:351496/149‑1 (MQ=255)
                                                                                                                              tgtgcgcATGGGCGCCCTGGCCTCCCATGCGCACACCTTCAAGCGACGGGTCGCCACCCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCT‑GCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCt                    >  6:103387/7‑149 (MQ=255)
                                                                                                                               ctgGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTc                     <  4:151095/148‑1 (MQ=255)
                                                                                                                               ctgATCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCt                    >  6:163713/1‑149 (MQ=255)
                                                                                                                                          aGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTa         <  3:46497/149‑1 (MQ=255)
                                                                                                                                            gCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGt       >  2:70569/1‑149 (MQ=255)
                                                                                                                                             ccAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTGAGGGGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGGCCGGCTTTAGTg      <  1:201076/149‑1 (MQ=255)
                                                                                                                                                gggCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGt    >  1:106198/1‑148 (MQ=255)
                                                                                                                                                 ggCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGtgc  >  6:362561/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACAAGGTTGGCGCAAAGAACGCGCCTTCAGCCACGCCTTCGCCCCGCGGGGCAAAGCCATCGCTGGCGCCGAACAGCTGGTCACAACTCTGCAGCAGGCTGCGCACCCGCTCGCCGACATCGCGCTGCTGGTCATGGGAGGCCAGGGCGCCCATGCGCACACCTTCAAGCGACGGGTCGCCAACCACGACCTTGCTCAGGCGCTCGCGCAGGCGGGTGGCAACGGCATCCAGGTGCCTGGCCGGGACGATCGCACGGCGTATGGCGGTGCACTTCTGTCCGGCTTTAGTGGTCA  >  NC_002947/3704843‑3705136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑