Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,109 T→A 6.5% K162* (AAG→TAG) ‡ paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,1090TA 6.5% 185.9 / 2.3 61K162* (AAG→TAG) ‡paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base T (31/26);  new base A (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

TCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCAC  >  NC_002947/3717962‑3718255
                                                                                                                                                   |                                                                                                                                                  
tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTg                                                                                                                                                   <  4:358999/149‑1 (MQ=255)
       gTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCGTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCggg                                                                                                                                            <  1:356621/149‑1 (MQ=255)
              ggctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGcc                                                                                                                                     <  3:351309/149‑1 (MQ=255)
                        cgAGGTGGCGGGCGTGGGTGAGGGCTTCATGACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCGTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAgcgc                                                                                                                           <  2:127347/149‑1 (MQ=255)
                            gTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGgca                                                                                                                       <  8:319667/149‑1 (MQ=255)
                             tGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGgcag                                                                                                                      >  8:169328/1‑149 (MQ=255)
                               gcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGgcagca                                                                                                                    <  5:123869/149‑1 (MQ=255)
                                       gggTGAGGGCTTCATCACGCAGCGCTGCAGCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTa                                                                                                            <  7:282344/149‑1 (MQ=255)
                                               gCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgccg                                                                                                      >  7:209288/1‑147 (MQ=255)
                                               gCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgccg                                                                                                      >  8:207679/1‑147 (MQ=255)
                                                           aGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAg                                                                                        >  5:279036/1‑149 (MQ=255)
                                                                      atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                                              >  4:112198/1‑148 (MQ=255)
                                                                      atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                                              <  3:81852/148‑1 (MQ=255)
                                                                         caccacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGgcag                                                                                                                      >  4:176518/1‑105 (MQ=255)
                                                                         caccacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGgcag                                                                                                                      <  3:176518/105‑1 (MQ=255)
                                                                          accacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAg                                                                           >  6:44683/1‑147 (MQ=255)
                                                                            cacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAgg                                                                       >  7:266639/1‑149 (MQ=255)
                                                                               gcgGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCt                                                                    >  6:358932/1‑149 (MQ=255)
                                                                                 ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                                  <  8:130160/149‑1 (MQ=255)
                                                                                  gTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGa                                                                 <  5:358932/149‑1 (MQ=255)
                                                                                      aTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGa                                                              >  4:331306/1‑148 (MQ=255)
                                                                                         aGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGCTCTTGCAGAAGGCCTGGATGAAACt                                                          >  7:302290/1‑149 (MQ=255)
                                                                                           cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCTGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                                  <  3:325177/139‑1 (MQ=255)
                                                                                           cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                                  >  4:325177/1‑139 (MQ=255)
                                                                                             cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAACCTGGcc                                                      >  8:315597/1‑149 (MQ=255)
                                                                                              ccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCg                                                     >  3:108028/1‑149 (MQ=255)
                                                                                                aTTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAg                                                   <  3:112198/149‑1 (MQ=255)
                                                                                                    cTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                                               >  2:264746/1‑149 (MQ=255)
                                                                                                    cTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                                               >  1:49493/1‑149 (MQ=255)
                                                                                                    cTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgg                                                <  7:207679/148‑1 (MQ=255)
                                                                                                     tCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCgggcgggc                                              >  8:131257/1‑149 (MQ=255)
                                                                                                       ggCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggcg                                             <  6:279036/148‑1 (MQ=255)
                                                                                                        gCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                                           <  8:285373/149‑1 (MQ=255)
                                                                                                             ttCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGCCCGCTCGGCCAgtt                                      <  2:103250/149‑3 (MQ=255)
                                                                                                               cGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCAc                                     >  8:356584/1‑148 (MQ=255)
                                                                                                               cGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCAc                                     <  1:264746/148‑1 (MQ=255)
                                                                                                               cGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGACAGCAc                                     <  7:169328/148‑1 (MQ=255)
                                                                                                                   gcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGt                                >  8:319385/1‑149 (MQ=255)
                                                                                                                     cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAg                                  <  6:265225/145‑1 (MQ=255)
                                                                                                                     cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAg                                  >  5:265225/1‑145 (MQ=255)
                                                                                                                     cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                              >  7:204802/1‑149 (MQ=255)
                                                                                                                     cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                              >  7:232724/1‑149 (MQ=255)
                                                                                                                       aGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCa                            <  8:209288/149‑1 (MQ=255)
                                                                                                                         gcgcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAgg                          >  6:38594/1‑149 (MQ=255)
                                                                                                                             tCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGcc                        >  6:214727/1‑147 (MQ=255)
                                                                                                                               gCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAgg                    <  7:185981/149‑1 (MQ=255)
                                                                                                                               gCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAgg                    <  5:38594/149‑1 (MQ=255)
                                                                                                                                 tcggCATGGCCCGGGCCAAGGCGCTGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCaa                      <  5:211937/142‑1 (MQ=255)
                                                                                                                                 tcggCATGGCCCGGGCCAAGGCGCTGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCaa                      >  8:351535/4‑145 (MQ=255)
                                                                                                                                 tcggCATGGCCCGGGCCAAGGCGCTGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCaa                      <  7:351535/142‑1 (MQ=255)
                                                                                                                                 tcggCATGGCCCGGGCCAAGGCGCTGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCaa                      >  6:211937/4‑145 (MQ=255)
                                                                                                                                 ccAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg                     >  8:214430/1‑146 (MQ=255)
                                                                                                                                 ccAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg                     <  7:214430/146‑1 (MQ=255)
                                                                                                                                           cAGCGCCTTGGCCCGGGCCATGCCGCCCAAGCGCGGCAGCCGCCAAGTACCGCCGGCGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGGCAAGGGAATGTTggcac        >  3:120837/1‑149 (MQ=255)
                                                                                                                                           cAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTgg           <  4:148672/146‑1 (MQ=255)
                                                                                                                                           cAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTgg           >  3:148672/1‑146 (MQ=255)
                                                                                                                                            aGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggcacc       >  4:311486/1‑149 (MQ=255)
                                                                                                                                              cgcCTTGGCCAGGGCCATGCCGACCAAGCGCTGCAGCAGCGAAGGACCGCCGGCGTCCGGCACCAGGCCGATGGTGCCGAAGGGCTGGATGAAACTGGCCGCGCGGGCGGCCGGCCCAGGGTCGCAGGCAAGGGGAATGTTgtcgccgg     >  8:211242/1‑149 (MQ=255)
                                                                                                                                                 cTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGc                                                                      >  4:256132/1‑81 (MQ=255)
                                                                                                                                                 cTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGc                                                                      <  3:256132/81‑1 (MQ=255)
                                                                                                                                                 cTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCGGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGACAAGGGAATGTTggcaccggcac  >  3:257595/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCAC  >  NC_002947/3717962‑3718255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑