Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	333,181	A→C	7.4%	E107A (GAG→GCG)	PP_5435 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	333,181	0	A	C	7.4%	205.2 / 6.6	68	E107A (GAG→GCG)	PP_5435	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base C (3/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCC  >  NC_002947/333034‑333329                                                                                                                                                    |                                                                                                                                                      tCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAg                                                                                                                                                      >  8:300656/1‑149 (MQ=255)   ccccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGt                                                                                                                                                     <  1:43111/148‑1 (MQ=255)    cccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                   >  3:226647/1‑149 (MQ=255)    cccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                   >  5:189215/1‑149 (MQ=255)     ccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTCCGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGTCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACg                                                                                                                                                  <  5:124163/149‑1 (MQ=255)     ccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                   >  2:256277/1‑148 (MQ=255)      cACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGa                                                                                                                                                 <  7:300656/149‑1 (MQ=255)         tCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑a                                                                                                                                              >  7:152712/1‑148 (MQ=255)         tCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑a                                                                                                                                              >  6:329347/1‑148 (MQ=255)           aaaaCTCAGTCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGGCTGGCGCAAAGCGCGACCGGGGACTCTCTACAGCAACGCCATGTTGTCCCACGCCGAGTACGCC‑AAAt                                                                                                                                           >  4:233072/1‑149 (MQ=255)           aaaaCTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAAt                                                                                                                                           <  7:301676/149‑1 (MQ=255)           aaaaCTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAAt                                                                                                                                           >  8:284278/1‑149 (MQ=255)               cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                           >  6:286292/1‑144 (MQ=255)               cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                           >  6:281200/1‑144 (MQ=255)               cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                           <  5:286292/145‑2 (MQ=255)               cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                           <  5:281200/145‑2 (MQ=255)                  aGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTg                                                                                                                                       >  6:309161/1‑146 (MQ=255)                   gccgctccAGCCGCAAACGGCCGCTTGATATCTCCGTGTTCGATATCAAGAAAAACGTATGCCGCCTGATCGCGGGGCTCATTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGa                                                                                                                                   <  8:278632/143‑1 (MQ=255)                    gCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAg                                                                                                                                    >  1:347571/1‑147 (MQ=255)                      ccGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGc                                                                                                                                >  1:150630/1‑149 (MQ=255)                      ccGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGc                                                                                                                                >  1:3703/1‑149 (MQ=255)                         ccAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGa                                                                                                                              >  5:316189/1‑148 (MQ=255)                             ccGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCa                                                                                                                         <  8:168240/149‑1 (MQ=255)                               gCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                   <  1:326184/122‑1 (MQ=255)                               gCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                   >  2:326184/1‑122 (MQ=255)                                 aaaGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                                     <  8:164736/149‑1 (MQ=255)                                           ttGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTggg                                                                                                           >  7:136552/1‑149 (MQ=255)                                             gATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAAAGCGCGAACGGGTACTCTCTACATAAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTTCAGAAGCGATATCAGGTAGCAACCTTGGcc                                                                                                         >  1:104594/1‑149 (MQ=255)                                               tatCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCcgc                                                                                                       <  6:189215/149‑1 (MQ=255)                                                     cGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCTAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCcgcgc                                                                                                     <  7:284278/145‑1 (MQ=255)                                                     cGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGata                                                                                                 <  8:147701/149‑1 (MQ=255)                                                         ttCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                                     >  2:26654/1‑125 (MQ=255)                                                         ttCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                                     <  1:26654/125‑1 (MQ=255)                                                            gATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCaa                                                                                           <  1:256277/148‑1 (MQ=255)                                                            gATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCaa                                                                                           <  4:226647/148‑1 (MQ=255)                                                                  aaGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGTTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACa                                                                                    <  2:7317/149‑1 (MQ=255)                                                                  aaGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACa                                                                                    <  2:3703/149‑1 (MQ=255)                                                                   aGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAg                                                                                   <  5:309161/149‑1 (MQ=255)                                                                         aCGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGCGTACTCTCTACATCAAGGCCATTTTGTCGCACGCGGAGTACGAC‑AAATCGTGCAGAAGCGATATCATGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGac                                                                              >  1:155453/1‑148 (MQ=255)                                                                          cGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGacaa                                                                            <  8:136552/149‑1 (MQ=255)                                                                                  gccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGc                                                                    <  3:128897/149‑1 (MQ=255)                                                                                  gccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGc                                                                    <  4:369305/149‑1 (MQ=255)                                                                                   ccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCg                                                                   <  2:347571/149‑1 (MQ=255)                                                                                    cTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCgg                                                                  <  8:152712/149‑1 (MQ=255)                                                                                       aTCGCGTGGCTGAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCggg                                                                 <  3:350051/147‑1 (MQ=255)                                                                                         cgcgTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATa                                                              >  6:167832/1‑148 (MQ=255)                                                                                             tGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATc                                                         <  6:316189/149‑1 (MQ=255)                                                                                                cTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                      <  8:344236/149‑1 (MQ=255)                                                                                                cTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                      <  4:335619/149‑1 (MQ=255)                                                                                                   aaTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAAt                                                                                          <  8:351784/110‑1 (MQ=255)                                                                                                   aaTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGc                                                    <  5:329347/148‑1 (MQ=255)                                                                                                           aaCGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                      <  8:239511/138‑1 (MQ=255)                                                                                                           aaCGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                      >  7:239511/1‑138 (MQ=255)                                                                                                                gTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATg                                      >  2:80783/1‑149 (MQ=255)                                                                                                                   ctctctACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCt                                   >  3:313143/1‑149 (MQ=255)                                                                                                                      tctACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCa                                >  7:327407/1‑149 (MQ=255)                                                                                                                        tACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGat                                                                                                                             >  1:299040/1‑54 (MQ=255)                                                                                                                        tACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGat                                                                                                                             <  2:299040/54‑1 (MQ=255)                                                                                                                          cATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCAt                               <  4:313143/146‑1 (MQ=255)                                                                                                                             cAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                         >  5:371428/1‑149 (MQ=255)                                                                                                                                  accatttGTCGTACTCGGCGTGCGACAAAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCAACGCTAATAtt                     >  2:15467/5‑149 (MQ=255)                                                                                                                                   cATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATAttt                    >  4:175260/1‑148 (MQ=255)                                                                                                                                    aTTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                         >  6:345549/1‑142 (MQ=255)                                                                                                                                    aTTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                         <  5:345549/142‑1 (MQ=255)                                                                                                                                            gCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCt          >  5:120510/1‑149 (MQ=255)                                                                                                                                                gCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGa                                             >  6:224101/1‑110 (MQ=255)                                                                                                                                                gCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGa                                             <  5:224101/110‑1 (MQ=255)                                                                                                                                                gCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGAt      >  8:83511/1‑149 (MQ=255)                                                                                                                                                    aGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGcc  <  1:80783/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCC  >  NC_002947/333034‑333329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑