Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	333,184	A→G	7.3%	Y108C (TAC→TGC)	PP_5435 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	333,184	0	A	G	7.3%	194.0 / 4.9	68	Y108C (TAC→TGC)	PP_5435	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base G (3/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

CCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCT  >  NC_002947/333037‑333330                                                                                                                                                    |                                                                                                                                                    cccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                    >  3:226647/1‑149 (MQ=255) cccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                    >  5:189215/1‑149 (MQ=255)  ccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTCCGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGTCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACg                                                                                                                                                   <  5:124163/149‑1 (MQ=255)  ccACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                    >  2:256277/1‑148 (MQ=255)   cACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGa                                                                                                                                                  <  7:300656/149‑1 (MQ=255)      tCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑a                                                                                                                                               >  7:152712/1‑148 (MQ=255)      tCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑a                                                                                                                                               >  6:329347/1‑148 (MQ=255)        aaaaCTCAGTCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGGCTGGCGCAAAGCGCGACCGGGGACTCTCTACAGCAACGCCATGTTGTCCCACGCCGAGTACGCC‑AAAt                                                                                                                                            >  4:233072/1‑149 (MQ=255)        aaaaCTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAAt                                                                                                                                            >  8:284278/1‑149 (MQ=255)        aaaaCTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAAt                                                                                                                                            <  7:301676/149‑1 (MQ=255)            cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                            >  6:286292/1‑144 (MQ=255)            cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                            >  6:281200/1‑144 (MQ=255)            cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                            <  5:286292/145‑2 (MQ=255)            cTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGTACTCGGCGTGCGAC‑aaaa                                                                                                                                            <  5:281200/145‑2 (MQ=255)               aGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTg                                                                                                                                        >  6:309161/1‑146 (MQ=255)                gccgctccAGCCGCAAACGGCCGCTTGATATCTCCGTGTTCGATATCAAGAAAAACGTATGCCGCCTGATCGCGGGGCTCATTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGa                                                                                                                                    <  8:278632/143‑1 (MQ=255)                 gCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAg                                                                                                                                     >  1:347571/1‑147 (MQ=255)                   ccGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGc                                                                                                                                 >  1:150630/1‑149 (MQ=255)                   ccGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGc                                                                                                                                 >  1:3703/1‑149 (MQ=255)                      ccAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGa                                                                                                                               >  5:316189/1‑148 (MQ=255)                          ccGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCa                                                                                                                          <  8:168240/149‑1 (MQ=255)                            gCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                    >  2:326184/1‑122 (MQ=255)                            gCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTAc                                                                                                                                                    <  1:326184/122‑1 (MQ=255)                              aaaGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                                      <  8:164736/149‑1 (MQ=255)                                        ttGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTggg                                                                                                            >  7:136552/1‑149 (MQ=255)                                          gATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAAAGCGCGAACGGGTACTCTCTACATAAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTTCAGAAGCGATATCAGGTAGCAACCTTGGcc                                                                                                          >  1:104594/1‑149 (MQ=255)                                            tatCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCcgc                                                                                                        <  6:189215/149‑1 (MQ=255)                                                  cGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCTAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCcgcgc                                                                                                      <  7:284278/145‑1 (MQ=255)                                                  cGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGata                                                                                                  <  8:147701/149‑1 (MQ=255)                                                      ttCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                                      <  1:26654/125‑1 (MQ=255)                                                      ttCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTa                                                                                                                      >  2:26654/1‑125 (MQ=255)                                                         gATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCaa                                                                                            <  4:226647/148‑1 (MQ=255)                                                         gATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCaa                                                                                            <  1:256277/148‑1 (MQ=255)                                                               aaGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGTTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACa                                                                                     <  2:7317/149‑1 (MQ=255)                                                               aaGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACa                                                                                     <  2:3703/149‑1 (MQ=255)                                                                aGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAg                                                                                    <  5:309161/149‑1 (MQ=255)                                                                      aCGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGCGTACTCTCTACATCAAGGCCATTTTGTCGCACGCGGAGTACGAC‑AAATCGTGCAGAAGCGATATCATGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGac                                                                               >  1:155453/1‑148 (MQ=255)                                                                       cGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGacaa                                                                             <  8:136552/149‑1 (MQ=255)                                                                               gccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGc                                                                     <  3:128897/149‑1 (MQ=255)                                                                               gccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGc                                                                     <  4:369305/149‑1 (MQ=255)                                                                                ccTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCg                                                                    <  2:347571/149‑1 (MQ=255)                                                                                 cTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCgg                                                                   <  8:152712/149‑1 (MQ=255)                                                                                    aTCGCGTGGCTGAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCggg                                                                  <  3:350051/147‑1 (MQ=255)                                                                                      cgcgTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATa                                                               >  6:167832/1‑148 (MQ=255)                                                                                          tGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATc                                                          <  6:316189/149‑1 (MQ=255)                                                                                             cTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                       <  4:335619/149‑1 (MQ=255)                                                                                             cTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                       <  8:344236/149‑1 (MQ=255)                                                                                                aaTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAAt                                                                                           <  8:351784/110‑1 (MQ=255)                                                                                                aaTGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGc                                                     <  5:329347/148‑1 (MQ=255)                                                                                                        aaCGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                       <  8:239511/138‑1 (MQ=255)                                                                                                        aaCGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGaa                                                       >  7:239511/1‑138 (MQ=255)                                                                                                             gTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATg                                       >  2:80783/1‑149 (MQ=255)                                                                                                                ctctctACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCt                                    >  3:313143/1‑149 (MQ=255)                                                                                                                   tctACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCa                                 >  7:327407/1‑149 (MQ=255)                                                                                                                     tACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGat                                                                                                                              >  1:299040/1‑54 (MQ=255)                                                                                                                     tACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGat                                                                                                                              <  2:299040/54‑1 (MQ=255)                                                                                                                       cATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCAt                                <  4:313143/146‑1 (MQ=255)                                                                                                                          cAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                          >  5:371428/1‑149 (MQ=255)                                                                                                                               accatttGTCGTACTCGGCGTGCGACAAAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCAACGCTAATAtt                      >  2:15467/5‑149 (MQ=255)                                                                                                                                cATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATAttt                     >  4:175260/1‑148 (MQ=255)                                                                                                                                 aTTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                          >  6:345549/1‑142 (MQ=255)                                                                                                                                 aTTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTaa                          <  5:345549/142‑1 (MQ=255)                                                                                                                                         gCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCt           >  5:120510/1‑149 (MQ=255)                                                                                                                                             gCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGa                                              >  6:224101/1‑110 (MQ=255)                                                                                                                                             gCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGa                                              <  5:224101/110‑1 (MQ=255)                                                                                                                                             gCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGAt       >  8:83511/1‑149 (MQ=255)                                                                                                                                                 aGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGcc   <  1:80783/149‑1 (MQ=255)                                                                                                                                                  gTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCt  >  6:65458/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGAC‑AAATGGTGCAGAAGCGATATCAGGTAGCAACCTGGGCCGCGCGATATTTCAATTCGACAGGTGACAACATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCT  >  NC_002947/333037‑333330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑