Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,750,735	G→T	7.5%	H89N (CAT→AAT)	PP_3315 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,750,735	0	G	T	7.5%	196.6 / 4.2	62	H89N (CAT→AAT)	PP_3315	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (23/34);  new base T (3/2);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GCGCCAGCGAGCGCCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATAACCACCG  >  NC_002947/3750590‑3750881                                                                                                                                                   |                                                                                                                                                   gcgcCAGCGAGCGCCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGTATGCtt                                                                                                                                                 <  2:67081/149‑1 (MQ=255) gcgcCAGCGAGCGCCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGcgt                                                                                                                                                 >  3:262177/1‑147 (MQ=255) gcgcCAGCGAGCGCCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTAAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCtt                                                                                                                                                 <  1:231118/149‑1 (MQ=255)     cAGCGAGCGCCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAAt                                                                                                                                             >  5:208080/1‑149 (MQ=255)      agcgagcgCCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAAtt                                                                                                                                            <  4:283492/149‑1 (MQ=255)           gcgcCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATTCAAGCATTCCATc                                                                                                                                       <  2:215751/149‑1 (MQ=255)           gcgcCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATc                                                                                                                                       <  3:94175/149‑1 (MQ=255)            cgccgcGACTTTGGCCGGTCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGCAAAGACAACGCGCACGATGGAATGCTTGAATTCCATc                                                                                                                                       <  1:252596/149‑1 (MQ=255)            cgccgcGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATcg                                                                                                                                      <  6:208080/149‑1 (MQ=255)             gccgcGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATcgc                                                                                                                                     <  2:352055/149‑1 (MQ=255)                 cgACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATc                                                                                                                                 <  4:81173/149‑1 (MQ=255)                     tttGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATcag                                                                                                                               >  2:58903/1‑147 (MQ=255)                          ccGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTc                                                                                                                        <  5:47283/149‑1 (MQ=255)                          ccGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTc                                                                                                                        <  5:91387/149‑1 (MQ=255)                               cTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGAc                                                                                                                   <  8:210304/149‑1 (MQ=255)                               cTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGAc                                                                                                                   <  8:204753/149‑1 (MQ=255)                                  aCATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTcc                                                                                                                                          >  6:126593/1‑123 (MQ=255)                                    aTCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATAc                                                                                                              <  7:291015/149‑1 (MQ=255)                                    aTCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATAc                                                                                                              >  2:275198/1‑149 (MQ=255)                                    aTCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATAc                                                                                                              >  6:372520/1‑149 (MQ=255)                                      cGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATAcc                                                                                                             >  8:85717/1‑148 (MQ=255)                                               ggATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTc                                                                                                   <  7:85717/149‑1 (MQ=255)                                               ggATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTc                                                                                                   <  1:4777/149‑1 (MQ=255)                                                      gATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTgg                                                                                                >  3:71463/1‑145 (MQ=255)                                                            gcgcGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATc                                                                                          <  8:93351/145‑1 (MQ=255)                                                            gcgcGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCg                                                                                        <  6:312185/147‑1 (MQ=255)                                                                    gcgcGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGc                                                                              <  5:372520/149‑1 (MQ=255)                                                                      gcgATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCCTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCg                                                                             >  6:123085/1‑148 (MQ=255)                                                                         aTCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGCTGGACCACCGGAtc                                                                         <  5:63514/149‑2 (MQ=255)                                                                            aaCCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGaa                                                                      >  3:315008/1‑149 (MQ=255)                                                                            aaCCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGaa                                                                      <  2:81208/149‑1 (MQ=255)                                                                            aaCCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGCTGGACCACCGGAtcttc                                                                      <  1:58903/149‑5 (MQ=255)                                                                              cccGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATc                                                                                                                                       <  4:99268/82‑1 (MQ=255)                                                                              cccGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATc                                                                                                                                       >  3:99268/1‑82 (MQ=255)                                                                              cccGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAAc                                                                     >  3:103403/1‑148 (MQ=255)                                                                              cccGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACg                                                                    >  8:84586/1‑149 (MQ=255)                                                                              cccGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGCTGGACCACCGGAtcttcca                                                                    <  6:110462/149‑7 (MQ=255)                                                                                cGAGCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCa                                                                  <  4:44132/149‑1 (MQ=255)                                                                                    cAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAAc                                                                     <  5:160264/142‑1 (MQ=255)                                                                                    cAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAAc                                                                     >  6:160264/1‑142 (MQ=255)                                                                                                      cTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGa                                            >  8:338478/1‑149 (MQ=255)                                                                                                      cTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGa                                            >  7:191298/1‑149 (MQ=255)                                                                                                                ctgcCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGAt                                  <  7:84586/149‑1 (MQ=255)                                                                                                                   ccATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAg                               <  5:380843/149‑1 (MQ=255)                                                                                                                   ccATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAg                               <  1:11460/149‑1 (MQ=255)                                                                                                                   ccATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAg                               >  2:11460/1‑149 (MQ=255)                                                                                                                   ccATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGAAGGCTGAAGACTATCTGGATCAg                               >  6:380843/1‑149 (MQ=255)                                                                                                                    cATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGc                              <  1:275198/149‑1 (MQ=255)                                                                                                                        gcgc‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGt                           <  2:77495/148‑1 (MQ=255)                                                                                                                         cgc‑AAGAGAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATAGCGAAGACATTCTGGAAGATGCGGTGGTCCAGCGGATTGATCGCATCGATGATCAGCTGGCTGAAGACTATCTGGATCAGCTGTAc                         <  5:123085/149‑1 (MQ=255)                                                                                                                                  aaCGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGaca                 >  5:32044/1‑149 (MQ=255)                                                                                                                                  aaCGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGaca                 >  1:303398/1‑149 (MQ=255)                                                                                                                                    cgcgCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAgg                                                   >  5:77151/1‑113 (MQ=255)                                                                                                                                    cgcgCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAgg                                                   <  6:77151/113‑1 (MQ=255)                                                                                                                                        cACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGa           <  4:71463/149‑1 (MQ=255)                                                                                                                                          cGATGGAATTCAAGCATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGAt          >  1:60897/1‑148 (MQ=255)                                                                                                                                          cGATGGAATTCAAGCATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGAt          >  1:150491/1‑148 (MQ=255)                                                                                                                                          cGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGAt          >  2:310310/1‑148 (MQ=255)                                                                                                                                           gATGGAATTCAAGCATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATaa        >  6:109899/1‑149 (MQ=255)                                                                                                                                                aaTTCTTGAAATCCATCGCGATCAGCAGCGTCATGACCAAACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATAaccacc   <  6:32044/149‑1 (MQ=255)                                                                                                                                                aaTGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATAaccacc   <  2:303398/149‑1 (MQ=255)                                                                                                                                                 aTGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATAACCACcg  >  3:119515/1‑149 (MQ=255)                                                                                                                                                   gCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATAac       <  1:37935/142‑1 (MQ=255)                                                                                                                                                   gCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATAac       >  2:37935/1‑142 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCGCCAGCGAGCGCCGCGACTTTGGCCGGGCTGACATCGGGATCAAGGATCACGATCTTGCGCGACAGCGCGATCAACCCGATCAAGAGGACCGTCTTGACCTGAATGATGCTGCCATGGCGC‑AAGACAACGCGCACGATGGAATGCTTGAATTCCATCGCGATCAGCAGCGTCATGACCATACCGAACACATTCTGGAAGATCCGGTGGTCCAGCGGATTGAACGCATCGATGATCAGCAGGCTGAAGACTATCTGGATCAGCTGTACCAGCGACACCACGATAACCACCG  >  NC_002947/3750590‑3750881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑