Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	334,194	C→T	5.4%	N15N (AAC→AAT)	PP_0275 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	334,194	0	C	T	5.4%	222.2 / 2.4	74	N15N (AAC→AAT)	PP_0275	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base T (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

TAGATTCGCTCCTCTTGGCATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTGAAGCAAGAACTGA  >  NC_002947/334048‑334342                                                                                                                                                   |                                                                                                                                                     tAGATTCGCTCCTCTTGGCATCCATCCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACCAATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGACGTTGACCGAGTCGGTACGGGCGGGTGCGATTGAGCTAATCaacaacct                                                                                                                                                    >  8:191939/1‑147 (MQ=255)  aGATTCGCTCCTCTTGGCATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGc                                                                                                                                                   >  2:286051/1‑149 (MQ=255)   gATTCGCTCCTCTTGGCATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCa                                                                                                                                                  >  2:144264/1‑149 (MQ=255)    aTTCGCTCCTCTTGGCATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAg                                                                                                                                                 >  2:251841/1‑149 (MQ=255)      tCGCTCCTCTTGGCATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAACTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGcc                                                                                                                                               >  5:83245/1‑149 (MQ=255)         ctcctcTTGGCATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCGGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGAGCAAGTCGTTGACCGTGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTg                                                                                                                                            <  8:29582/149‑1 (MQ=255)                   cATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGTTTACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATg                                                                                                                                  >  1:268361/1‑149 (MQ=255)                    aTCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCTTACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGc                                                                                                                                 >  1:285138/1‑149 (MQ=255)                       cATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCgt                                                                                                                               <  8:349723/148‑1 (MQ=255)                        aTGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCgtgt                                                                                                                             <  3:27733/149‑1 (MQ=255)                                cccAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGc                                                                                                                      >  6:287679/1‑148 (MQ=255)                                 ccAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGAt                                                                                                                                   <  5:45281/134‑1 (MQ=255)                                 ccAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGAt                                                                                                                                   >  6:45281/1‑134 (MQ=255)                                   aaTACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCaaa                                                                                                                  <  5:287679/149‑1 (MQ=255)                                       cTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTg                                                                                                              <  7:138739/149‑1 (MQ=255)                                        tGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAAc                                                                                                                                                      <  5:110543/108‑1 (MQ=255)                                        tGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAAc                                                                                                                                                      >  6:110543/1‑108 (MQ=255)                                        tGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTg                                                                                                              >  1:29243/1‑148 (MQ=255)                                          cATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCTTTTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGc                                                                                                             <  1:298667/147‑1 (MQ=255)                                             ctcGCCGTGGCTGCGACGCATACAGTATTGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGTCGGGTGCGATTGAGATAATCATCAACGGACGCCCTGGATTCCGATGCGTGTATGTGGCCAAATTTGAATACa                                                                                                        >  4:40044/1‑149 (MQ=255)                                               cGCCGAGGCTCCGACGCAAACAGTATGGCCAGCACCCAGAGCGCCCGATCAAGTCGGGGACCGAGTCGGTCGGGCCGGCTGCGATTGAGCTCATCATCAACGGCAGCCCTGCGTTCCGATGCGTGTATGTGGCCAAATTTGCAGACATg                                                                                                      <  6:376959/149‑1 (MQ=255)                                                gCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATg                                                                                                      <  4:335371/148‑1 (MQ=255)                                                gCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCCGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGAATGAGCTCATAATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATgg                                                                                                     >  5:364303/1‑149 (MQ=255)                                                       cTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTcgtcgt                                                                                              <  1:251841/149‑1 (MQ=255)                                                        ttcGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATGAAGTCGTTGACCGAGTGGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCgtg                                                                                             <  7:191939/147‑1 (MQ=255)                                                        tGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCgtg                                                                                             >  5:361122/1‑149 (MQ=255)                                                          cGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTAGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGtt                                                                                           <  3:206448/149‑1 (MQ=255)                                                          cGACGCATACAGGATGGCCAGGCCCCAGCGCGGCCGAGCAAGGCGGTGACCGCGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCACCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCgtggt                                                                                           <  6:83245/149‑3 (MQ=255)                                                            aCGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTg                                                                                          >  3:235224/1‑148 (MQ=255)                                                               cATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCAtt                                                                                      <  1:170392/149‑1 (MQ=255)                                                                aTACAGTATTGCCAGGACCCAGAGCGCCCGATCAAGTAGTTGACCGAGTCGGACGGGGCGGGAGCGATTGAGCTCATCATCAATGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATCGTCGTCGTGTTGCAttc                                                                                     >  6:102522/1‑149 (MQ=255)                                                                aTACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACAGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCAttc                                                                                     >  1:74798/1‑149 (MQ=255)                                                                   cAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCAttcgt                                                                                   <  7:288350/148‑1 (MQ=255)                                                                    aGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGTTCGTCGTGTTGCAttcgttc                                                                                 >  7:122185/1‑149 (MQ=255)                                                                     gTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGc                                                                                         >  3:92268/1‑140 (MQ=255)                                                                     ctagggCCAGGACCCAGAGCGGCCGATCAAGGCGTTGACCGAGGCGGTCGGGGCGGGTTCGATTGAGCTCATCATCAACGCCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGc                                                                                         <  4:92268/136‑1 (MQ=255)                                                                      tATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATtcgttcgt                                                                               >  3:341778/1‑149 (MQ=255)                                                                       attgacAGGACCCAGAGAGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATtcgttcgt                                                                               <  8:122185/143‑1 (MQ=255)                                                                         ggCCAGGAACCAGAGAGGCCTATCAAGTCGTAGACCGAGTCGGTCGTGGCGGGTGCGATTGAGCTCATCATCAACGGCAGACCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGa                                                                             <  3:40044/148‑1 (MQ=255)                                                                          gCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGaa                                                                            >  5:204401/1‑148 (MQ=255)                                                                           ccAGGACCCAGAGCGGCCGTTCTAGTCGTTGACCGAGTCGGTCGGGGGGGGTGCGATTGAGCTGATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGaaaa                                                                          <  6:364303/149‑1 (MQ=255)                                                                            cAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAAc                                                                         >  2:37721/1‑149 (MQ=255)                                                                            cAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAAc                                                                         <  1:286051/149‑1 (MQ=255)                                                                            cAGGAACCAGAGCGGTGGTTCATGTCGTTGACCGTGTTGGTGGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCGCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTTAAAAc                                                                         <  4:335583/149‑1 (MQ=255)                                                                              ggACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCAAAATTTGCAGACATCTTCGTCGTGTTGCATTCGTTCGTGAAAACGa                                                                       <  6:106256/149‑1 (MQ=255)                                                                                  ccAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGc                                                                                                                                 >  8:271056/1‑87 (MQ=255)                                                                                  ccAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGc                                                                                                                                 <  7:271056/87‑1 (MQ=255)                                                                                  ccAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCaa                                                                   >  8:149595/1‑149 (MQ=255)                                                                                            cGATCATTTCGTTGACCGAGTGGGTCGGGGCGGGTGCGTTTGAGCTCTTCATCAATGGCAGGCGTGCATTCCGATGGGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGGATTCGTTCGTGAAAACGACCAACAGGTCCgta                                                         <  5:102522/149‑3 (MQ=255)                                                                                            cGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGaa                                                         <  6:204401/149‑1 (MQ=255)                                                                                             gATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAATGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAAc                                                        >  6:145388/1‑149 (MQ=255)                                                                                             gATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCAttcgttc                                                                                 <  5:226487/124‑1 (MQ=255)                                                                                             gATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCAttcgttc                                                                                 >  6:226487/1‑124 (MQ=255)                                                                                             gATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAAc                                                        >  3:45449/1‑149 (MQ=255)                                                                                             gATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAAc                                                        <  6:361122/149‑1 (MQ=255)                                                                                             gATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAAc                                                        >  2:92092/1‑149 (MQ=255)                                                                                               tCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACgg                                                      <  1:144264/149‑1 (MQ=255)                                                                                                cAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTgg                                                                                                                       <  8:30879/83‑1 (MQ=255)                                                                                                   gTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCAcg                                                  <  4:235224/149‑1 (MQ=255)                                                                                                      cccgACCGACTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGa                                               >  4:97089/4‑149 (MQ=255)                                                                                                           ccGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGgtc                                                                                                   <  8:877/92‑1 (MQ=255)                                                                                                           ccGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGgtc                                                                                                   >  7:877/1‑92 (MQ=255)                                                                                                               gtcggtcgGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCg                                      >  8:108281/1‑149 (MQ=255)                                                                                                                   gtcggGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCg                                   >  7:245986/1‑148 (MQ=255)                                                                                                                   gtaggggCGGGTGCGATAGAGCTCATCATAAACGGCAGCCCTGCATTACGATGCGTGTATTTGGCCAAATTTGCAGAAATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGACCGCGATGCAGGTCGACg                                   >  2:4279/4‑148 (MQ=255)                                                                                                                      ggggCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACa                               <  4:341778/149‑1 (MQ=255)                                                                                                                        ggCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGc                             <  2:268361/149‑1 (MQ=255)                                                                                                                              tGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGaa                                                         <  1:153314/115‑1 (MQ=255)                                                                                                                              tGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGaa                                                         >  2:153314/1‑115 (MQ=255)                                                                                                                                 gATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCAc                                                   <  7:158759/118‑1 (MQ=255)                                                                                                                                 gATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACACGTCCGAACGGCAc                                                   >  8:158759/1‑118 (MQ=255)                                                                                                                                      aGCTCATCATCAATGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGtt                <  5:145388/148‑1 (MQ=255)                                                                                                                                        cTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTg               >  4:48676/1‑147 (MQ=255)                                                                                                                                           atcatcaACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTGAAGc           >  4:178492/1‑148 (MQ=255)                                                                                                                                            tcatcaACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTGAAGCaa         <  2:29243/149‑1 (MQ=255)                                                                                                                                                  aCGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTGAAGCAAGAACt    >  4:358490/1‑148 (MQ=255)                                                                                                                                                  aCGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCAGAACGCCAAGCGATGCAGGTCGCAGAACAGCGGATGAAGGAGATGAACCAAGAACt    >  3:18347/1‑148 (MQ=255)                                                                                                                                                   cGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTGAAGCAAGAACTga  <  2:285138/149‑1 (MQ=255)                                                                                                                                                   cGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGAACAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTGAAGCAAGAACTga  <  4:45449/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TAGATTCGCTCCTCTTGGCATCCATGCCGTCCCCAATACTGCATCTCGCCGTGGCTGCGACGCATACAGTATGGCCAGGACCCAGAGCGGCCGATCAAGTCGTTGACCGAGTCGGTCGGGGCGGGTGCGATTGAGCTCATCATCAACGGCAGCCCTGCATTCCGATGCGTGTATGTGGCCAAATTTGCAGACATGGTCGTCGTGTTGCATTCGTTCGTGAAAACGACCAACAGGTCCGAACGGCACGCGATGCAGGTCGCCGAACAGCGGATGAAGGAGTTGAAGCAAGAACTGA  >  NC_002947/334048‑334342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑