BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,788,398	G→C	5.7%	Y79* (TAC→TAG)	PP_3350 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,788,398	0	G	C	5.7%	340.7 / 7.9	106	Y79* (TAC→TAG)	PP_3350	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (46/54); new base C (2/4); total (48/58)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.79e-01

Only 100 of 106 total aligned reads displayed.

GGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCCCGGC  >  NC_002947/3788251‑3788542

ggCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGt                                                                                                                                                 <  1:352157/149‑1 (MQ=255)

     gggTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCgg                                                                                                                                            <  7:294675/149‑1 (MQ=255)

     gggTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCgg                                                                                                                                            <  2:204413/149‑1 (MQ=255)

         aTTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCt                                                                                                                                        >  7:7348/1‑149 (MQ=255)

           tctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCt                                                                                                                                        >  6:373253/1‑147 (MQ=255)

            ctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCa                                                                                                                                      <  7:49153/148‑1 (MQ=255)

            ctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccgat                                                                                                                                      <  8:74372/148‑4 (MQ=255)

             tcGACCTGGAAGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAga                                                                                                                                    >  7:171869/1‑148 (MQ=255)

               gACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccgatg                                                                                                                                     <  1:265270/146‑5 (MQ=255)

               gACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccgatg                                                                                                                                     <  8:315065/146‑5 (MQ=255)

                  cTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGcccc                                                                                                                                 >  5:380786/1‑147 (MQ=255)

                     gACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                                                                                                            <  6:113828/149‑1 (MQ=255)

                     gACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                                                                                                            >  7:9807/1‑149 (MQ=255)

                     gACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGt                                                                                                                             <  3:17146/148‑1 (MQ=255)

                       cGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGc                                                                                                                          <  2:61466/149‑1 (MQ=255)

                        gAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTg                                                                                                                           >  7:68597/1‑147 (MQ=255)

                        gAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCa                                                                                                                         <  4:35030/149‑1 (MQ=255)

                        gAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCa                                                                                                                         <  2:370828/149‑1 (MQ=255)

                          aGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAgt                                                                                                                       >  5:138752/1‑148 (MQ=255)

                           gggaCGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAgtc                                                                                                                      <  3:139016/146‑3 (MQ=255)

                                gCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGcc                                                                                                                 >  8:194596/1‑149 (MQ=255)

                                  aCGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCg                                                                                                                >  5:303395/1‑148 (MQ=255)

                                  aCGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGa                                                                                                               >  3:272919/1‑149 (MQ=255)

                                      cATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGActct                                                                                                           <  1:280815/149‑1 (MQ=255)

                                       aTCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTg                                                                                                          <  8:7348/149‑1 (MQ=255)

                                          gtcgAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCTTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACa                                                                                                       <  6:273751/149‑1 (MQ=255)

                                          gtcgAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACa                                                                                                       >  7:288616/1‑149 (MQ=255)

                                          gtcgAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACa                                                                                                       <  5:373253/149‑1 (MQ=255)

                                           tcgAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTCCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAg                                                                                                      >  8:211140/1‑149 (MQ=255)

                                             gAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGcc                                                                                                    <  6:125248/149‑1 (MQ=255)

                                             gAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccgatgc                                                                                                                                    >  4:151376/1‑112 (MQ=255)

                                             gAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccgatgc                                                                                                                                    <  3:151376/117‑6 (MQ=255)

                                              aaCAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGcc                                                                                                    >  5:170434/1‑148 (MQ=255)

                                               aCAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGAc                                                                                                              <  3:282129/137‑1 (MQ=255)

                                               aCAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGAc                                                                                                              >  4:282129/1‑137 (MQ=255)

                                                cAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGAt                                                                                                 >  7:184308/1‑149 (MQ=255)

                                                 aGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATg                                                                                                <  8:68597/149‑1 (MQ=255)

                                                  ggTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATgg                                                                                               >  7:79895/1‑149 (MQ=255)

                                                    tGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTa                                                                                             <  6:117030/149‑1 (MQ=255)

                                                        ggTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAgcgc                                                                                         <  1:41003/149‑1 (MQ=255)

                                                         gTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAgcg                                                                                          >  5:157627/1‑147 (MQ=255)

                                                           cGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAgg                                                                                      <  6:157627/149‑1 (MQ=255)

                                                             gCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGACGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAgga                                                                                     >  6:127690/1‑147 (MQ=255)

                                                              cATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCGAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATg                                                                                                <  8:308978/136‑1 (MQ=255)

                                                              cATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCGAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATg                                                                                                >  7:308978/1‑136 (MQ=255)

                                                                 gggCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGa                                                                                                  >  7:148687/1‑131 (MQ=255)

                                                                 gggCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGa                                                                                                  <  8:148687/131‑1 (MQ=255)

                                                                     cTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGCCAGCCGATGGTAGCGCAGGCCAGCGAAAg                                                                            >  4:245675/1‑149 (MQ=255)

                                                                     cTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAg                                                                            >  1:291394/1‑149 (MQ=255)

                                                                        cAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAggg                                                                          <  3:28109/148‑1 (MQ=255)

                                                                         aGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGc                                                                        <  2:291394/149‑1 (MQ=255)

                                                                              ttGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTgg                                                                   >  1:329757/1‑149 (MQ=255)

                                                                                  tgcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCt                                                                                                                                        >  1:223129/1‑76 (MQ=255)

                                                                                  tgcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCtt                                                               >  3:49292/1‑149 (MQ=255)

                                                                                   gcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTg                                                              >  1:121586/1‑149 (MQ=255)

                                                                                     aGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCAt                                                                                  <  7:319482/127‑1 (MQ=255)

                                                                                     aGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCAt                                                                                  >  8:319482/1‑127 (MQ=255)

                                                                                      gATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCg                                                           <  1:225504/149‑1 (MQ=255)

                                                                                      gATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCAGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCg                                                           >  5:320579/1‑149 (MQ=255)

                                                                                       aTAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGGCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCgg                                                          >  8:244528/1‑149 (MQ=255)

                                                                                       aTAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCgg                                                          >  4:12139/1‑149 (MQ=255)

                                                                                         aGGATTCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCTACTCTTACAGCCGATGGTAGCGCAGGCCATc                                                                                 <  1:40248/124‑1 (MQ=255)

                                                                                         aGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCgggg                                                        >  3:138920/1‑149 (MQ=255)

                                                                                         aGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAACGGTTAGTCCGAAGCATCGGCCCGGTAGCGGGACTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGAAAGACGATGGTATCGCAGGCCATc                                                                                 >  2:40248/1‑124 (MQ=255)

                                                                                          ggAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCgggg                                                        <  7:194596/148‑1 (MQ=255)

                                                                                          ggAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCgggg                                                        <  8:288616/148‑1 (MQ=255)

                                                                                           gAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAgg                                                                           <  7:316329/128‑1 (MQ=255)

                                                                                           gAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAgg                                                                           >  8:316329/1‑128 (MQ=255)

                                                                                           gAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTg                                                                    >  6:340818/1‑135 (MQ=255)

                                                                                              tcgtcgTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGgtcgtc                                                   >  1:233649/1‑149 (MQ=255)

                                                                                                gtcgtTCAGCCCGGGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCga                                                 <  4:65517/149‑1 (MQ=255)

                                                                                                gtcgtTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGgtcgtc                                                   >  8:10167/1‑147 (MQ=255)

                                                                                                       aGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGATGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCTGTCAGGCCGACTCTGACAGGCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCg                                          <  2:213636/149‑1 (MQ=255)

                                                                                                       aGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCg                                          <  6:303395/149‑1 (MQ=255)

                                                                                                        gCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                         >  5:10523/1‑149 (MQ=255)

                                                                                                        gCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                         >  2:201925/1‑149 (MQ=255)

                                                                                                         cgcgcgCATGCCAAAGAAGGGTTTGTCCGATGCATCGGCCGGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                         <  5:106104/146‑1 (MQ=255)

                                                                                                         cccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                         >  1:69891/1‑148 (MQ=255)

                                                                                                         cccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGt                                        <  4:272919/149‑1 (MQ=255)

                                                                                                          ccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTa                                       <  8:143796/149‑1 (MQ=255)

                                                                                                          ccGCGCAGGCCAAAGAAGGGTTAGACAGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCAACGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCgg                                         >  7:235527/1‑147 (MQ=255)

                                                                                                             cgcAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTc                                    <  5:127690/149‑1 (MQ=255)

                                                                                                             cgcAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTc                                    <  7:278634/149‑1 (MQ=255)

                                                                                                              gcAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTcc                                   <  2:69891/149‑1 (MQ=255)

                                                                                                                aGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTc                                 <  8:79895/149‑1 (MQ=255)

                                                                                                                  gCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCgaga                                               >  7:299452/1‑133 (MQ=255)

                                                                                                                  gCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCgaga                                               <  8:299452/133‑1 (MQ=255)

                                                                                                                    cAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGa                               >  2:342728/1‑147 (MQ=255)

                                                                                                                    cAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGa                               <  1:342728/147‑1 (MQ=255)

                                                                                                                            ggTTTGTCCGAAGCATGGGCCCGGTAGCGGGCCTCAGCCCCGTTTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAg                      <  8:235527/148‑1 (MQ=255)

                                                                                                                               ttGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGc                  <  7:244528/149‑1 (MQ=255)

                                                                                                                                    cGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCt              <  6:138752/148‑1 (MQ=255)

                                                                                                                                    cGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTg             >  5:339085/1‑149 (MQ=255)

                                                                                                                                    cGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTg             >  8:41525/1‑149 (MQ=255)

                                                                                                                                        gCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCt         >  1:207986/1‑149 (MQ=255)

                                                                                                                                          tgaggCCCGCTACCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTcc       >  8:235440/4‑149 (MQ=255)

                                                                                                                                          aTCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTcc       <  3:12139/149‑1 (MQ=255)

                                                                                                                                           tCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTccc      <  6:170434/149‑1 (MQ=255)

                                                                                                                                              gCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCccgg   >  4:207329/1‑149 (MQ=255)

                                                                                                                                               cccGGTAGCGGGCCTCAGCCCCGAGTTGCAGTCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGGGGGGTCGTCGTGAATGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCccggc  <  2:329757/149‑1 (MQ=255)

GGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCCCGGC  >  NC_002947/3788251‑3788542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`